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Showing 1 to 20 of 47 for “"cDNA clone"”.
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Studies of the Expression of Chloroperoxidase: Isolation and Sequence of a Chloroperoxidase Cdna Clone (complementary-Dna)
… details the isolation of a chloroperoxidase cDNA clone and the study of the mechanisms which control the expression of chloroperoxidase.
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Characterization of cDNA and Genomic Clones for a Palmitoyl-acyl Carrier Protein Thioesterase and an Osmotin-Like PR5 Protein in Gossypium Hirsutum.
Putative cotton cDNA clones and cognate genomic clones for a palmitoyl-acyl carrier protein (ACP) thioesterase (PATE) and an osmotin-like pathogenesis-related 5 (PR5) protein have been isolated and characterized. PATE is a class B fatty acid thioesterase with specificity for saturated long-chain …
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Molecular characterization and promoter analysis of the maize cytosolic glyceraldehyde 3-phosphate dehydrogenase gene family
… emphasis on gpc4. Analysis of full length cDNA clones for GAPC2, GAPC3 and GAPC4 revealed that the deduced amino acid sequence of GAPC4 has 99.4% identity with the GAPC3 cDNA clone as compared to only 81% with either GAPC1 or GAPC2. Northern blot analysis revealed a maximum transcript …
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Palmitoyl-acyl Carrier Protein Thioesterase in Cotton (Gossypium hirsutum L.): Biochemical and Molecular Characterization of a Major Mechanism for the Regulation of Palmitic Acid Content
… and seed oil accumulation. A cotton FatB cDNA clone was isolated by screening a cDNA library with a heterologous Arabidopsis FatB probe (Pirtle et al., 1999, Plant and Cell Physiology 40: 155-163). The predicted amino acid sequence of the cotton PATE preprotein had 63% identity to the …
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Identification and Characterization of a Calmodulin -Binding Protein Related to a Family of ATPases Involved in Cell Division and Membrane Fusion
… transduced. An Arabidopsis thaliana L. flower cDNA expression library was screened for CaM-binding proteins with 35S-labeled CaM. A partial cDNA whose encoded protein shares a high level of similarity with yeast CDC48p was isolated. A genomic clone was isolated using the partial length cDNA …
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Isolation and characterization of starch, starch biosynthetic genes and protease inhibitors from Marama beab (Tylosema Esculentum)
… A complementary Deoxyribonucleic acid (cDNA) library was constructed from marama tuber for the screening and isolation of Soluble Starch Synthase I (SSSI) and a Polymersae Chain Reaction (PCR) based strategy was used to isolate Adenosine diphosphate-glucose pyrophosphorylase (AGPase) and …
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Γονιδιακή κλωνοποίηση μορίου κυτταρικής συνάφειας του νευρικού συστήματος
… LN-10 cell adhesion molecule (chick) has been cloned with the use of heterologous cDNA probe (rat). The LN-10 molecule is expressed specifically in the chick nervous system (cerebellum, optic lobus, hemispheres) and is absent from non-neuronal tissues (liver, blood, intestine). The expression …
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Molecular cloning and characterization of two Arabidopsis thaliana sulfotransferases
… as a query sequence. The intronless AtST4a was cloned via PCR from Arabidopsis thaliana ecotype Col-0 genomic DNA. A cDNA clone of AtST6 was obtained and fully sequenced. AtST4a and AtST6 were both cloned into the bacterial expression vector pQE30. The recombinant proteins expressed in E. coli …
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Cloning and sequencing of a β-glucosidase of cDNA from Sorghum bicolor (L.) Moench and analysis of expression in seedlings
A full-length β-glucosidase encoding cDNA is isolated and sequenced from Sorghum bicolor (L) Moench. Using 5’- and 3’- end specific probes derived from the cDNA clone, the multiplicity of β-glucosidase genes and their expression in different tissues were studied. Southern blotting data showed that …
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RNAi-related genes in Toxoplasma gondii.
… Argonaute protein (TgAgo) in T. gondii. The cDNA clone has an open reading frame (ORF) of 1575 bp which encodes a putative 524-amino-acid protein with a calculated molecular weight of 58.5 kDa and an estimated isoelectric point (pI) of 9.4. Using bioinformatic methods, we found significant …
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MOLECULAR BIOLOGY OF HEPATITIS B VIRUS DNA-CELL INTERACTION
… protein which binds to, and activates EII. The cDNA clone of the protein designated here as E2BP, was isolated from a ?gtll expression library constructed from the hepatoma cell line HuH-6 which was screened with a binding site probe derived from EII. Sequence analysis of E2BP revealed 86.6% …
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Identification and Characterization of a Tolerant Response in Arabidopsis Thaliana to Tobacco Ringspot Virus and Cloning of a Single Locus That Confers Tolerance
… with yeast artificial chromosome (YAC) clone CIC2A12, suggesting that this YAC clone contains the TTR1 locus. A cDNA clone, 120K3T7, also co-segregated with TTR1 in 430 F$\sb3$ families. Cosmid libraries of the YAC clone CIC2A12 (containing Col-0 TTR1 locus) and A. thaliana Estland …
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Cloning and regulation of rat and Xenopus laevis hepatic 3-hydroxy-3-methylglutaryl Coenzyme A reductase
The cDNAs coding for rat and Xenopus laevis 3-hydroxy-3-methylglutaryl Coenzyme A (HMG-CoA) reductase have been cloned and sequenced. The rat liver HMG-CoA reductase cDNA clone contains one open reading frame coding for 887 amino acids. Its 106 nucleotide long 5$\sp\prime$-noncoding region contains …
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Purification and Characterization of OTF-1, A Transcription Factor Regulating Cell Cycle Expression of a Human Histone H2b Gene: Demonstration of its Functional Identity with NF-III, a Factor Required for Efficient Initiation of Adenovirus DNA Replication
… data suggesting that we have isolated a cDNA clone for this factor are presented and discussed.</p>
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Molecular characterization of the cucumber necrosis virus coat protein gene
… site-directed mutagenesis of an infectious CNV cDNA clone, pK2/M5 (wild-type, 4701 nt). Transcripts of PD(-) were infectious in Nicotiana clevelandii, however, the resulting local lesions were significantly smaller than those on the corresponding leaves of plants inoculated with wild-type …
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Cloning and Estrogen Regulation of the Retinol Binding Protein Messenger-Rna of Xenopus Laevis
… I described the isolation of a full length cDNA clone for an estrogen induced mRNA isolated from the liver of Xenopus laevis. By DNA sequencing and subsequent homology alignment with the sequence for human retinol binding protein, the clone was identified as the mRNA for serum retinol …
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Isolation and Characterization of the Soybean Major Latex Homologue Msg, and Analysis of Its Pod -Specific Promoter
… disease resistance strategies. A random lambda cDNA clone, VS-107, isolated from a soybean pod library, showed elevated mRNA expression in soybean pods and it was used as a probe to retrieve the gene and promoter sequences from a soybean genomic lambda library. Computer analyses indicate that …
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Isolation and Characterization of the Soybean Major Latex Homologue Msg, and Analysis of Its Pod-Specific Promoter
… disease resistance strategies. A random lambda cDNA clone, VS-107, isolated from a soybean pod library, showed elevated mRNA expression in soybean pods and it was used as a probe to retrieve the gene and promoter sequences from a soybean genomic lambda library. Computer analyses indicate that …
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Polymorphism of bovine MHC class II genes: Association with persistent lymphocytosis caused by bovine leukemia virus
… cows, 13 of which were PL. A 783 bp BoLA-DQA cDNA clone $(\alpha$5) was identified by screening a bovine cDNA library. The $\alpha$5 clone has an average nucleotide similarity of 91.6%, 86.0% and 84.2% with a BoLA-DQA genomic clone, SLA-DQA, and HLA-DQA, respectively. In contrast, similarity …
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