Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 900 for “"cDNA"”.
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Human ceruloplasmin gene and cDNA
… and anti-coagulation modulation. The cDNA of ceruloplasmin sharehomology with the cDNAs of factors V and VIII of the blood coagulation cascade,suggesting that the three genes evolved from a common ancestral gene. Thereare two ceruloplasmin specific mRNA transcripts, 3.7 and 4.4 Kb, …
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Optimization of cDNA microarray image analysis methods
… expression levels on spotted complementary DNA (cDNA) microarray images. The aims of the present thesis are: a) to model and address the effects of cDNA microarray image noise in such a way that it will increase the accuracy of the extracted gene expression levels, b) to investigate the impact of …
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Identifizierung und Charakterisierung einer murinen Nphp1 cDNA
… eine zur humanen komplementären Nphp1 cDNA in einer cDNA Genbank identifiziert und charakterisiert. Durch die neu gewonnene cDNA wurden anschliessend in-situ-Hybridisierungsanalysen durchgeführt.
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Optimizing Conditions for Cloning cDNA from Chicken Brain
… gene expression techniques including cDNA synthesis and cloning were attempted first with the chicken brain. The initial attempts at making cDNA libraries were elusive; therefore, experiments were performed to identify problems and enhance the efficiency of specific steps. These steps …
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GeneSieve: A Probe Selection Strategy for cDNA Microarrays
… of thousands of genes simultaneously. Often, cDNA libraries representing expressed genes of an organism are available, along with expressed sequence tags (ESTs). ESTs are widely used as the probes for microarrays. Designing custom microarrays, rich in genes relevant to the experimental …
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Molecular Cloning of the cDNA for Porcine Parotid Hormone
… study was developed to isolate and identify the cDNA sequence for porcine parotid hormone.</p> <p>A porcine parotid cDNA library was constructed and hybridized with oligo probes designed based on the partial amino acid sequences of the isolated porcine parotid hormone. Over 100 positive clones …
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Methyl Chloride Transferase: Isolation, characterization,cDNA Cloning, and Expression
The genomic DNA of methyl chloride transferase of Batis maritima was also characterized and is composed of 8 exons and 7 introns.
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Critical Issues in the Processing of cDNA Microarray Images
… the gene expression levels, the processing of cDNA microarray images must overcome a large set of issues in these three phases that motivates this study. This study presents automatic gridding methods and compares their performances. Two segmentation techniques already used, the Seeded Region …
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Topics on statistical design and analysis of cDNA microarray experiment
… In this thesis, we focus on the dual channel cDNA microarray which is one of the most popular microarray technologies and discuss three different topics: optimal experimental design; estimating the true proportion of true nulls, local false discovery rate (lFDR) and positive false discovery …
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Genetic Profiling of the Bovine Pituitary Gland Using cDNA Microarray Technology
… of this dissertation were to 1) use custom-made cDNA microarrays to identify genes in the bovine pituitary gland that are differentially expressed during the estrous cycle and 2) characterize their patterns of gene expression. The estrous cycle is a dynamic process that requires coordination …
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Gene expression profiling of patients with Polycythaemia Rubra Vera using cDNA microarrays
… <br>pathogenesis of PV. This led us to perform cDNA microarray analysis of PV patients in order to get a more global view of gene expression in this myeloproliferative disorder. Granulocyte RNAs from 40 polycythaemia vera (PV) and 12 secondary erythrocytosis (SE) patients were analysed. …
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Retroviral-mediated gene transfer of Il-1β?cDNA into human activated T cells
… A modified human interleukin-1β (IL-Iβ) cDNA inserted into the MFG retroviral vector served as an indicator gene in these studies. Optimized methodologies resulted in T cell transduction frequencies of approximately 50-75%. An important consideration for the success of T lymphocyte-based …
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cDNA microarray Analysis Identifies Symptom-Associated Chemokine Genes in Behcet's Disease Mouse Model
… blood mononuclear cells from each group, a cDNA microarray was performed. microarray chips were composed of 7,744 mouse genes. The BD skin group was compared with the BD eye group, and BD inactive group. The genes expressed differently and we selected genes of related to inflammation out of …
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Characterization of XV7 and XV9 cDNA clones which confer osmotolerance to Eschericia coli
The sequence similarity between XV7 and the mammalian syntaxin-like protein kinase receptor shows that XV7 may function similar to that of animal protein kinases. The syntaxin-like protein kinase receptor was recently entered into the Genbank (Thoreau, unpublished), therefore not much is known …
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Stereoselective metabolism of verapamil and four other chiral drugs by cDNA expressed CYP450's.
… of verapamil and four other chiral drugs by cDNA expressed CYP450's.
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Protein purification and cDNA cloning of suGF1 : a sea urchin nuclear DNA-binding factor
The upstream regulatory regions of numerous genes contain contiguous deoxyguanosine residues (G·C-rich sequences) which have been implicated in the regulation of gene expression, since they may involve alterations in their DNA structure, the binding of G-string factors and in some cases even the …
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Aplicación de macroarrays de cDNA en estudios de expresión genética en frutos de prunus persica
La técnica de arreglos de DNA ofrece varias ventajas sobre otros métodos de estudio de la expresión genética, ya que permite cuantificar en forma relativamente rápida y reproducible la abundancia relativa de los transcritos expresados en un tejido sometido a una condición experimental determinada. …
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Generation of cDNA libraries of amoeba, 8 hour, and 12 hour stages of Dictyostelium discoideum
… responsible for activating gp-2 expression, cDNA plasmid libraries of amoebae, and cells at 8 h and 12 h of development were generated. The long-term goals of this project involve screening expression libraries with identified cis-acting elements from the gp-2 promoter to yield the DNA …
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