Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"cAMP-dependent protein kinase(PKA)"”.
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Identification of a Novel ERK 1/2-Interacting A-Kinase Anchoring Protein
… identified in Chlamydomonas, radial spoke protein 3 (RSP3) is one of at least twenty identified radial spoke structural components of motile cilia and is required for axonemal sliding and flagellar motility. The mammalian orthologs for this and other radial spoke proteins, however, remain …
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Redox Regulation of Type Iα PKA in the Heart
The two regulatory RIα subunits of cAMP-dependent protein kinase (PKA) form interprotein disulfides during oxidative stress which is associated with kinase activation. PKA classical activation involves cAMP binding to the regulatory subunits causing release of catalytic subunits which phosphorylate …
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Regulation of Molecular Composition at Glutamatergic Synapses
… Receptor, NMDAR) molecular markers in rat hippocampal cultured neurons. PROT localized specifically to presynaptic excitatory synapses. Immunostaining of PROT was detected several days following synapse formation, suggesting a regulatory function for the transporter. Approximately 60% of total …
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Structure-Function Studies of the cAMP-Dependent Protein Kinase <i>In Vitro</i> and in Intact Cells
<p>There are 518 protein kinase genes in the human genome; this constitutes about 1.7% of all human genes. The cAMP-dependent protein kinase (PKA) serves as the prototypic model for the study of kinases because it contains a conserved catalytic core shared with all eukaryotic kinases, it is the …
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The Roles of Ca2+ and cAMP in the Nematocyst Discharge of the Sea Anemone Tentacle
… the intracellular second-messenger, cyclic-AMP (cAMP). We now show that NANA dose-dependently increases<em> in situ</em> cAMP levels in the ectodermal layer of tentacles from <em>A. pallida</em>, but has no effect on the endodermal cAMP content. In addition, NANA activates cAMP-dependent protein …
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Cγ- and Cα-Subunits Provide the Specificity for cAMP/PKA Function
<p>The Cα and Cγ of the cAMP-dependent protein kinase (PKA) are two highly homologous (83% amino acid identity), yet functionally distinct isozymes <em>in vitro</em> for substrate and pseudosubstrate specificity, and in intact cells for cell phenotypes (Beebe, 1992).</p> <p>To determine the …
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Go 단백질의 알파 소단위체에 의한 신경돌기형성의 조절
Heterotrimeric G protein은 많은 신경전달물질과 호르몬에 의해서 일어나는 신호전달을 중개한다. 모든 G 단백질들 중에서, Go는 신경세포분화에 관여한다고 알려져 있지만 어떻게 중재하는지는 명확히 알려진 바가 없다. 이전 연구에서 Goα가 F11 세포에서 신경돌기진전(neurite outgrowth)을 조절한다는 것을 보았다. Goα가 과발현되는 세포에서 신경돌기의 평균길이는 감소하였으나 세포당 신경돌기의 수는 반대로 증가하였고 이러한 양상은 cAMP-PKA-CREB 신호에 의해 매개되었다. 본 연구에서는 …
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Novel peptide-based approaches to study the activity and substrate specificity of protein kinases
Protein phosphorylation, catalyzed by protein kinases, is the predominant post-translational modification made to proteins. This process is readily reversible, with protein phosphatases catalyzing removal of the installed phosphate groups. Together, protein kinases and phosphatases modulate the …