Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"cAMP-dependent protein kinase"”.
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Regulation of NeuroD/BETA2 by cAMP Dependent Protein Kinase A Signaling pathway
… 있다는 사실을 의미하기 때문이다. 이에 본 연구의 Part 1 에서는 NeuroD와cAMP-PKA경로가 신경세포에서 담당하는 역할의 중요성에 주목하였고, 그 결과 PKA에 의한 NeuroD의 인산화 과정을 최초로 확인하였다. PKA에 의한 NeuroD인산화의 정확한 위치 (T91, S137)를 규명하였고, PKA 매개 NeuroD 인산화가 가지는 세포 생리학적 의미와 그 분자 기전을 제시하였다. PKA에 의한 NeuroD의 인산화 위치는 정제된 NeuroD의 in vitro kinase assay와 Mass …
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Structure-Function Studies of the cAMP-Dependent Protein Kinase <i>In Vitro</i> and in Intact Cells
<p>There are 518 protein kinase genes in the human genome; this constitutes about 1.7% of all human genes. The cAMP-dependent protein kinase (PKA) serves as the prototypic model for the study of kinases because it contains a conserved catalytic core shared with all eukaryotic kinases, it is the …
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Protein phosphatases and protein kinases in dictyostelium
… behavior and partial characterization of 5 protein phosphatases, 6 paranitrophenyl phosphate (pnpp) phosphatases and 2 protein kinases from <i>Dictyostelium</i> are described. .Two of the protein phosphatases are shown to have subunit structures. All of the protein phosphatase activities …
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Protein Kinase A Regulates Hyphal Growth by Relieving the Interaction of MoSfl1 with the Cyc8-Tup1 Co-repressor in Magnaporthe oryzae
The cAMP-dependent protein kinase A (PKA) signal transduction pathway plays an important role in morphogenesis and virulence in plant pathogenic fungi. In the rice blast fungus Magnaporthe oryzae, it regulates surface recognition, appressorium turgor generation, and invasive growth. Two genes in M. …
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Micro-analysis of adenylate cyclase and cyclic AMP-dependent protein kinase activities in human mammary tumors
… the enzyme profiles of adenylate cyclase and cAMP-dependent protein kinase within a series of malignant and benign human mammary tumors. The methods allow for determination of enzyme activity in histologically-confirmed neoplastic areas within individual tumors which permits comparisons to be …
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Regulation Of Cyclic-amp-dependent Protein Kinases During Skeletal Myogenesis
… myotubes. The regulation of the levels of cAMP-dependent protein kinases during myogenesis in L6 was studied, as these enzymes have been proposed to be involved in the control of this process. Measurement of the mRNA levels for the type I regulatory subunit (R{dollar}sb{lcub}rm …
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Localization of Goα in the Developing and Adult Mouse Brain
Heterotrimeric G proteins mediate signal transduction generated by hormone and neurotransmitters. Among G proteins, Go, a member of the Gi/o family, is the most abundant heterotrimeric G protein in the brain but the roles and effectors for the Go have not been clearly defined. As previously …
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Identification of a Novel ERK 1/2-Interacting A-Kinase Anchoring Protein
… identified in Chlamydomonas, radial spoke protein 3 (RSP3) is one of at least twenty identified radial spoke structural components of motile cilia and is required for axonemal sliding and flagellar motility. The mammalian orthologs for this and other radial spoke proteins, however, remain …
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Regulation of SNARE-Mediated Synaptic Vesicle Release by Synaptotagmins and Complexins
… N-ethylmaleimide-sensitive-factor Attachment Protein Receptors) complex is the core component of the fusion machinery in the presynaptic terminal, as it forms a physical bridge between the vesicular membrane and the presynaptic target membrane that delivers the force to fuse the two membranes. …
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Redox Regulation of Type Iα PKA in the Heart
The two regulatory RIα subunits of cAMP-dependent protein kinase (PKA) form interprotein disulfides during oxidative stress which is associated with kinase activation. PKA classical activation involves cAMP binding to the regulatory subunits causing release of catalytic subunits which phosphorylate …
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Regulation of Molecular Composition at Glutamatergic Synapses
… Receptor, NMDAR) molecular markers in rat hippocampal cultured neurons. PROT localized specifically to presynaptic excitatory synapses. Immunostaining of PROT was detected several days following synapse formation, suggesting a regulatory function for the transporter. Approximately 60% of total …
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Characterization of the regulatory domain of the mammalian multifunctional De Novo pyrimidne biosynthetic enzyme CAD
… the first three activities i.e. the glutamine-dependent carbamoyl phosphate synthetase (CPSase), aspartate transcarbamoylase (ATCase), and dihydroorotase (DHOase) are carried on a single 240-kDa polypeptide chain called CAD. Carbamoyl phosphate synthetase (CPSase), the first enzyme in the …
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A study of the regulation of glycogen metabolism in Dictyostelium discoideum
… developmental cycle. Specifically, the possible cAMP dependent regulation of glycogen phosphorylase and glycogen synthase was examined. In other systems, cAMP can regulate at the level of the gene (procaryotes, CAP protein) or at the level of covalent, reversible modification of the enzyme …
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The Roles of Ca2+ and cAMP in the Nematocyst Discharge of the Sea Anemone Tentacle
… the intracellular second-messenger, cyclic-AMP (cAMP). We now show that NANA dose-dependently increases<em> in situ</em> cAMP levels in the ectodermal layer of tentacles from <em>A. pallida</em>, but has no effect on the endodermal cAMP content. In addition, NANA activates cAMP-dependent protein …
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Modulation of the aquaporin-2 localization in renal principal cells
… messenger 3’,5’-cyclic adenosine monophosphate (cAMP) and the activation of the cAMP-dependent protein kinase (protein kinase A, PKA), the water channel aquaporin-2 (AQP2) is redistributed from intracellular vesicles into the plasma membrane of renal collecting duct principal cells. Its insertion …
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Modulation of Signal Transduction in the Nervous System by Protein Phosphorylation
… brain function. This thesis examines the role of protein phosphorylation in mediating three different molecular mechanisms by which neurotransmitter receptors transduce their signals: chemically-gated ion channels, receptor-linked ion channels and receptor-linked ion pumps. The nicotinic …
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Go 단백질의 알파 소단위체에 의한 신경돌기형성의 조절
Heterotrimeric G protein은 많은 신경전달물질과 호르몬에 의해서 일어나는 신호전달을 중개한다. 모든 G 단백질들 중에서, Go는 신경세포분화에 관여한다고 알려져 있지만 어떻게 중재하는지는 명확히 알려진 바가 없다. 이전 연구에서 Goα가 F11 세포에서 신경돌기진전(neurite outgrowth)을 조절한다는 것을 보았다. Goα가 과발현되는 세포에서 신경돌기의 평균길이는 감소하였으나 세포당 신경돌기의 수는 반대로 증가하였고 이러한 양상은 cAMP-PKA-CREB 신호에 의해 매개되었다. 본 연구에서는 …
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Cγ- and Cα-Subunits Provide the Specificity for cAMP/PKA Function
<p>The Cα and Cγ of the cAMP-dependent protein kinase (PKA) are two highly homologous (83% amino acid identity), yet functionally distinct isozymes <em>in vitro</em> for substrate and pseudosubstrate specificity, and in intact cells for cell phenotypes (Beebe, 1992).</p> <p>To determine the …
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Investigation of the cAMP-mediated inhibitory mechanism on the signalling pathways of 2 cytokines: IL-6 and leptin in endothelial cells
… of the prototypical second messenger cyclic-AMP (cAMP), notably with regard to endothelial function. Many studies have shown that cAMP can limit vascular permeability by enhancing barrier function and reducing pro-inflammatory effects of cytokines. Although the protective effects of cAMP elevation …
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Regulation of cAMP-Dependent Gene Expression in Airway Epithelial Cells: A Transcriptional and Pharmacodynamic Analysis
… effective bronchodilators that work by elevating cAMP in airway smooth muscle (ASM). Less appreciated and understood is the ability of cAMP to regulate gene expression. For example, previous studies have shown LABAs to protect ASM against pro-contractile stimuli by up-regulating RGS2, which …
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