Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 51 for “"bulk RNA sequencing"”.
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Investigation of Sensory Cell-Specific Changes in Gene Expression in Microrna-183 Family Knockout Mouse Inner Ear From Bulk RNA Sequencing Analysis
… biology have emphasized the pivotal role of microRNAs in regulating gene expression during the development and maintenance of the inner ear. These small non-coding RNAs influence key biological processes such as cell differentiation, apoptosis, and oxidative stress, which are essential for …
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Modeling colorectal cancer initiation using human iPSC-derived intestinal organoids
… events associated with three oncogenes employing bulk-RNA sequencing to examine genetic expression and pathway commonalities compared to existing primary literature in the field of CRC. METHODS: A CDX2-GFP reporter hiPSC line was transfected independently with three constructs each containing a …
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Characterisation of human enteroendocrine cells in organoid models
… of fluorescence-activated cell sorting (FACS), bulk RNA sequencing, gut hormone secretion assays, single cell Ca²⁺ imaging, immunohistochemistry and peptidomics. GLU-Venus ileal organoids were first used to explore the mechanisms regulating GLP-1 release from human L-cells. We optimised …
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Insights into the piRNA pathway through 16 strains of mice
… several classes of non-coding regulatory RNAs. One such class of small RNAs - PIWI-interacting RNA (piRNAs) - are exclusively expressed in the germlines of mammals. This thesis provides an exhaustive bioinformatic exploration of piRNA biogenesis and dynamics utilising a large …
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Extracellular Vesicles as Mediators of Communication Between Endothelial Cells and Fibroblasts
… transferring regulatory molecules such as microRNAs (miRNAs) to nearby or distant cells. This study investigated whether EVs secreted by human dermal microvascular endothelial cells (EC-EVs) influence fibroblast behavior. We isolated and characterized EC-EVs and confirmed their uptake by primary …
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Functional analysis of amyotrophic lateral sclerosis mutations using Drosophila cell culture models
… providing potential therapeutic targets for ALS. Bulk RNA sequencing was employed to investigate transcriptomic changes in Drosophila S2R+ cells expressing TDP43 wild-type and mutant. Differential gene expression and pathway analysis revealed dysregulation in RNA metabolism, mitochondrial …
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Autocrine prolactin signaling in breast cancer cells leads to metabolic changes and therapeutic opportunities for anti-tumour and anti-bone metastatic treatment
… and respond to hPRL via signal transduction. Bulk RNA sequencing revealed that they express genes that suggest a more aggressive phenotype than MCF7-WT (WT) and empty vector (EV) control cells. It was also observed that they proliferate more than the empty vector control cells and that they …
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MULTI-OMIC LINEAGE TRACING PREDICTS THE MOLECULAR DRIVERS OF CANCER STEM CELLS IN TRIPLENEGATIVE BREAST CANCER EVOLUTION.
… and chromatin accessibility profiling (via snRNA-seq and snATAC-seq) and integrates these with phenotypic assays. Applying GALILEO to the intrinsically heterogeneous and highly tumorigenic TNBC cell line SUM159PT, we identified two distinct subpopulations: drug-tolerant cells (DTCs) and …
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Molecular characterisation of the role of systemic infections in Alzheimer’s disease brain
… cortex samples from the UK Brain Bank Network, bulk RNA sequencing revealed that systemic infection elicits distinct molecular responses in AD compared with controls. Differential expression and co-expression network analyses identified infection-dependent alterations in immune, vascular, and …
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Exploring the immune landscape of the tumour microenvironment in meningiomas and the role of immune infiltration in meningioma models
… Multiplex immunohistochemistry and bulk RNA sequencing were used to characterise the TME in genetically stratified meningioma tissues and their matched 3D spheroid models. Comparative analyses of immune cell infiltration were conducted across parental tissues, 2D monolayer cultures, …
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Lineage-Specific Phenotypic Abnormalities in iPSC-Derived Smooth Muscle Cells from Type-I Loeys-Dietz Syndrome
… LDS. Phenotypic characterisation, such as by bulk RNA sequencing, revealed that LM-SMCs have abnormal phenotypes, including impaired TGF-β signalling, irregular expression of extracellular matrix, contractility defect, inability to regulate cell proliferation, poor cellular motility, and …
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Intratumoral B and T cell receptors: reconstruction and analysis
… profiling protocol to original single-cell RNA sequencing (scRNA-seq) data obtained from melanoma patients, I present a complete computational reconstruction of intratumoral immune receptors in this cancer type. The scRNA-seq results are consistent with the presence of an ongoing …
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Organizer regeneration and patterning of stem cell lineages in planarians
… we characterized the planarian epidermis. Bulk RNA-sequencing of mature epidermal cells and subsequent in situ validation identified numerous spatial patterns. To understand when these patterns arose during differentiation, we performed single-cell sequencing (SCS) of epidermal progenitors. …
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The development, cytoarchitecture, and circuitry of the ventral lateral geniculate nucleus
… and map its connectivity with the retina. Using bulk RNA sequencing and proteomics, we identified extracellular matrix proteins that form two molecularly distinct types of perineuronal nets in two major laminae of vLGN: the retinorecipient external vLGN (vLGNe) and the non‐retinorecipient …
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Dissecting the interplay between cytotoxic T lymphocytes and the pancreatic cancer stroma
… contributions to be distinguished. In parallel, bulk RNA sequencing and targeted perturbations were used to identify candidate pathways underlying CTL non-activation and tumour cloaking in the PDAC–CAF co-culture system. Our findings suggest that CAFs drive a progressive shift from chemotactic to …
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Neutrophil localization during C. rodentium-induced colitis
… counterpart upon infection. Furthermore, the bulk RNA sequencing results show that NET formation and degranulation are upregulated neutrophil functions during C. rodentium infection in both cathelicidin-deficient and wildtype mice. By further understanding the behaviour and activity of …
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Effects of a high cholesterol diet on the pathogenesis of influenza A virus infection in mature mice
… neutralizing antibody levels in the blood. Bulk RNA sequencing was performed on the lungs to evaluate the effect of diet on the lung transcriptome during infection. Diet-induced changes to immune cell populations were determined by flow cytometry. Finally, a crossover design experiment was …
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Neoblast specialization during regeneration of the planarian S. mediterranea
… how heterogeneous the neoblast population is. A bulk RNA sequencing approach with expression screening identified 33 new transcription factors transcribed in specific differentiated cells that were also expressed in small fractions of neoblasts during regeneration. Transcription factors of …
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Exploring the function and sensing mechanisms of human enteroendocrine cells using intestinal organoids
… fluorescence-activated cell sorting (FACS), bulk RNA sequencing, hormone secretion assays and live single-cell Ca2+ and cAMP imaging. cAMP imaging was possible through the generation of K- and EC cell transgenic lines expressing genetically encoded cAMP reporters (Epac-S-H187). Bulk RNA …
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Single cell transcriptomics of the retinal vascular endothelium in health and diabetes
… in diabetes have been studied by PCR and bulk RNA sequencing methods. These methods give the impression of uniform expression across all cells within a sample; however, great variability has been found in gene expression between individual cells even within a relatively homogeneous …
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