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Showing 1 to 8 of 8 for “"brush border membranes"”.
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Preparation and Use of Bovine Jejunal Brush Border Membrane Vesicles to Investigate Amino Acid Uptake (Nutrient, Ruminant, Absorption, Cattle, Transport)
Purified brush border membranes were obtained from homogenized jejunal epithelial cells of slaughtered cattle by divalent cation aggregation of non-brush border membranes and differential centrifugation. Membrane marker enzyme assays determined effectiveness of the fractionation procedure. Compared …
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Non-electrolyte transport in brush border membrane vesicles from bovine small intestine
Transport properties of bovine intestinal brush border membranes were investigated. Isolation of brush border membrane vesicles involved magnesium precipitation followed by a sucrose density gradient. Characterization by alkaline phosphatase activity (the brush border marker enzyme) showed 7 fold …
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Preparation of brush border and basolateral membrane vesicles from bovine intestine for nutrient uptake studies
Brush border and basolateral membrane vesicles were isolated by subjecting homogenized mucosal cells from bovine small intestine to a divalent cation aggregation followed by a series of differential and density gradient centrifugations. Membrane marker enzyme assays were used to monitor the …
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Expression and glycosylation of meprin isoforms
… oligomeric metalloendopeptidases in renal brush border membranes. Meprin A contains 90-kDa subunits (α subunits) and is expressed in random-bred and some inbred strains of mice. Meprin B contains subunits of 110 kDa (β subunits) <i>in situ</i>, and the enzyme from C3H/He mice, a strain that …
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Binding mechanism of K88ab pili produced by enterotoxigenic Escherichia coli
Binding of K88ab pili by brush border membrane and mucus from pig small intestine was characterized by inhibition assay and Western blot. In Western blot, K88ab pili were bound by two major brush border membrane polypeptides with molecular weight of 61,500 and 57,000 in addition to numerous minor …
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Identification and characterization of a receptor for Clostridium Difficile enterotoxin
… C. difficile enterotoxin (toxin A) exists in the brush border membranes (BBMs) of the hamster, an animal known to be extremely sensitive to the action of the toxin. Toxin A was the only antigen adsorbed by the BBMs from the culture filtrate of C. difficile. Erythrocytes from various animal species …
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Structure, membrane association, and processing of meprin subunits
… expressed at high concentrations in the renal brush border membranes of mice. Meprins consist of two types of subunits, α and β, which are the products of two different genes. The β subunit cDNA was cloned and sequenced from mouse kidneys, and the membrane association and the in vivo …
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Nherf1 – New Modifier of Colorectal Cancer Progression
Colorectal cancer (CRC) develops from multiple progressive modifications of normal intestinal epithelium into adenocarcinoma. Loss of cell polarity has been implicated as an early event in this process, but the molecular players involved are not well known. NHERF1 (Na+/H+ Exchanger Regulatory …