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Showing 1 to 13 of 13 for “"brush border membrane vesicles"”.
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Characterization of methionine transport in bovine intestinal brush border membrane vesicles
Characteristics of methionine uptake by brush border membrane vesicles of bovine small intestine were investigated. Alkaline phosphatase marked the brush border membrane fraction obtained through differential centrifugation followed by a sucrose gradient. This preparation yielded a 10-fold …
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Non-electrolyte transport in brush border membrane vesicles from bovine small intestine
Transport properties of bovine intestinal brush border membranes were investigated. Isolation of brush border membrane vesicles involved magnesium precipitation followed by a sucrose density gradient. Characterization by alkaline phosphatase activity (the brush border marker enzyme) showed 7 fold …
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Characterization of Glycyl-Sarcosine Uptake by Ovine Intestinal Brush Border Membrane Vesicles
… from five sheep was studied through the use of brush border membrane vesicles (BBMV). Preliminary experiments determined that incubation in hyaluronidase is not necessary in order to separate mucosal tissue from the basement membrane and that the stop solution used in the uptake study needed to …
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Lysine and methionine transport by bovine jejunal and ileal brush border membrane vesicles
Purified brush border and basolateral membranes were isolated from homogenized intestinal enterocytes of Holstein steers by divalent cation precipitation followed by differential and sucrose density gradient centrifugation. Membrane marker enzymes were used to determine the effectiveness of the …
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Isolation of hog renal brush border membrane vesicles with application to the study of cadmium nephrotoxicity
… small intestine, consists of two distinct plasma membrane surfaces referred to as the brush border, or luminal surface, and the basal-lateral, or contraluminal surface. Once isolated, membrane vesicles formed from the kidney cell can serve as models for the study of heavy metal mephrotoxin …
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Methionine and glucose transport by isolated intestinal brush border membrane vesicles from pigs and lambs fed an Aspergillus product
… of methionine and glucose by isolated intestinal brush border membrane vesicles (BBMV). In Experiment 1, 24 weanling pigs were paired by sex, BW, and litter and were allotted, within pairs, to either an 18% CP corn-soy diet (control) or the same diet supplemented (.15%) with an Aspergillus …
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Preparation and Use of Bovine Jejunal Brush Border Membrane Vesicles to Investigate Amino Acid Uptake (Nutrient, Ruminant, Absorption, Cattle, Transport)
Purified brush border membranes were obtained from homogenized jejunal epithelial cells of slaughtered cattle by divalent cation aggregation of non-brush border membranes and differential centrifugation. Membrane marker enzyme assays determined effectiveness of the fractionation procedure. Compared …
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Urinary excretion of trace metals in the streptozotocin-diabetic rat
… of zinc binding and transport by renal brush border membrane vesicles (BBMV) in vitro. The accumulation of zinc by BBMV was found to be temperature dependent. No apparent differences in the binding and intravesicular accumulation of zinc by brush border membrane vesicles prepared from …
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Preparation of brush border and basolateral membrane vesicles from bovine intestine for nutrient uptake studies
Brush border and basolateral membrane vesicles were isolated by subjecting homogenized mucosal cells from bovine small intestine to a divalent cation aggregation followed by a series of differential and density gradient centrifugations. Membrane marker enzyme assays were used to monitor the …
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Placental P-glycoprotein: Role in disposition of medications administered during pregnancy
… efflux transporters in the placental apical membrane are involved in placental elimination of compounds and thus fetal protection. The goal of this investigation was to evaluate developmental and genetic influences on expression and activity of the placental efflux transporter P-glycoprotein …
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Influence of infant milk formula processing on protein digestion and gut barrier health in vitro and in vivo
… in our laboratory, a split-stream process using membrane filtration technology with a ceramic 1.4 µm microfiltration membrane and a 0.2 µm polyvinylidene difluoride polymeric membrane was employed to produce an IMF (MEM-IMF) with significantly higher levels of native whey protein compared to a …
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Molecular characterization of cation-coupled transporters: the H+-coupled Mg2+-citrate transporter, CitM, and the Na+/sulfate cotransporter, hNaSi-1
… inhibited by ionophores that affect the transmembrane proton gradient, including FCCP, TCC and nigericin, suggesting a proton-coupled transport. Valinomycin did not affect the uptake by CitM, supporting an electroneutral transport model in which one proton is coupled to the uptake of one …