Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"broadly neutralizing antibodies"”.
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HIV-1 Immune Escape and Neutralizing Antibodies : Solid Phase Proteoliposomes Containing HIV-1 Envelope Glycoproteins as Antigens and Immunogens and HIV-1 Core Envelope Glycoproteins Deficient in T-Helper Epitopes
… as trimeric spikes and are the major targets for neutralizing antibodies. The design of envelope glycoprotein-based subunit vaccines has been frustrated by many viral immune escape mechanisms. Trimeric envelope glycoprotein formulations hold promise to overcome limitations of monomeric envelope …
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Proteins Inside Cells and On the Surface of Membranes: Developing Three Dimensional In-Cell NMR and Structural Characterization of a Trimeric Membrane Proximal External Region Construct from HIV-1 gp41
… it contains the epitopes for several of the broadly neutralizing antibodies against HIV-1. In addition to studies of the monomeric peptide, we have developed a new trimeric MPER construct, designated gp41-M-MAT, that associates with detergent micelles and lipid bilayers. This construct is a …
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Genetic and immunological characterization of new subtype G envelope expressing HIV-1 pseudoviruses
… An ideal vaccine would elicit the production of broadly neutralizing antibodies (nAb), capable of having potent activity against worldwide viral isolates. This thesis encompasses a series of studies on characterizing the genetic diversity of envelope genes of subtype G and the neutralization …
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Evaluating the neutralizing antibody response to HIV-1 membrane proximal external regional; Implications for vaccine design
Inducing broadly neutralizing antibodies targeting the HIV-1 envelope is thought to be crucial for developing an effective vaccine. The Membrane Proximal External Region (MPER) within the HIV- 1 gp41 envelope is a promising vaccine target. The MPER is highly conserved, functionally constrained, …
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Humanized Mouse with a Diverse Polyclonal B Cell Repertoire as a Model for Neutralizing Antibody Responses against EBV and HIV
… has been historically difficult to model human neutralizing B cell responses to these two viruses using small animals. I leveraged a transgenic mouse model that encodes a genetically human antibody repertoire to generate neutralizing monoclonal antibodies (mAbs) against the EBV gp350 and gp42 …
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The Design And Synthesis Of Inhibitors Of Hiv-1 Viral Entry And Photoaffinity Labeled Probes For Structural Elucidation Of The Hiv-1 Env
… the Env conformation recognized by most broadly neutralizing antibodies. Importantly, no structure of this Env conformation is reported in the literature. This work is expected to enable both structural elucidation of this critical Env conformation and aid the development of an HIV-1 …
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Integrative approaches to decipher influenza evolution, antibody responses, and AI-driven specificity prediction
… identifying critical residues in IGHV1-69 broadly neutralizing antibodies that target the hemagglutinin (HA) stem. Chapter 4 showcases AI-driven models for predicting antibody specificity and highlights their promise for therapeutic design. Chapter 5 synthesizes key findings and outlines …
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Development and Characteristics of the Earliest Cross-Neutralizing Antibody Response to HIV-1
A neutralizing antibody response of sufficient potency, magnitude and duration is considered an important part of a successful HIV vaccine. A better understanding of the factors associated with the development of broadly neutralizing antibody responses (effective against a wide range of clinical …
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HIV-1 strain-specific neutralizing antibody responses and the dynamics of viral evolution
… sterilizing immunity, it would need to elicit broadly neutralizing antibodies (bnAbs). However, factors underlying the development of these antibodies are not clear. There is evidence to suggest that in some individuals who develop bnAbs, the development of breadth is influenced by the …
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HIV Specific B Cell Response in Patients With Broadly Neutralizing Serum Activity
<p>Antibodies to conserved epitopes on the HIV surface protein gp140 can protect against infection in non-human primates, suggesting that vaccines that elicit such antibodies would be protective. Some HIV infected individuals develop high titers of broadly neutralizing IgG antibodies in their …
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Design and characterization of novel immunogens for AIDS vaccine development and evaluation of a sample inference method for NGS Illumina amplicon data
… causes in 2015. A vaccine that could induce broadly neutralizing antibodies (bnAbs) is hypothesized to be the most efficient way to halt the AIDS pandemic. However, the majority of attempts to elicit bnAbs with HIV-1 vaccine candidates have failed due to the extensive variability and complex …
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Efficacy of Antibody and T cell Therapies for Highly Mutable Viruses like Human Immunodeficiency
The isolation of broadly neutralizing antibodies (bnAbs) that can neutralize diverse strains of highly mutable viruses like human immunodeficiency virus (HIV) as well as identification of mutationally-constrained regions of the proteome that could be targeted by T cells has led to interest in …
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Mapping Genotype to Phenotype with High-Throughput Empirical Approaches
… protein, we considered the binding landscape of broadly neutralizing antibodies (bnAbs) that confer protection against diverse influenza strains. Our understanding of the evolutionary pathways leading to bnAbs, and thus how best to elicit them, remains limited. We measure binding affinities of …
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Computational Study of Vaccination Strategies to Change B Cell Immunodominance
… the immunodominant epitopes and render existing antibodies ineffective. Moreover, as the immune system preferentially targets immunodominant epitopes, functionally important and conserved epitopes are less targeted, which poses a barrier to the generation of broadly neutralizing antibodies. …
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Effects of galectins and antibodies in HIV infection; Novel Assays
… to the limited host immune control. Still, broadly neutralizing antibodies (bnAbs) are found in some chronically infected HIV-infected individuals, which has spurred the research on antibody-based vaccines. An important tool in detecting and studying bnAbs, are neutralization assays. Here we …
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A statistical mechanics approach to vaccination against HIV
Most vaccines stimulate the production of antibodies that provide a potent defense upon reinfection by the same strain of pathogen. The key process in antibody development is a stochastic process known as affinity maturation (AM) which generates strain-specific antibodies upon immunization by one …
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Modulating the Antigenicity and Immunogenicity of HIV-1 Envelope by a Conserved N-Linked Glycan
… conserved sites that are targets for some broadly neutralizing antibodies (bNAbs). Previous work has shown that removal of a highly conserved potential N-linked glycan (PNLG) site at amino acid residue 197 (N7) on the surface antigen gp120 of HIV-1 envelope glycoprotein (Env) increases …
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Intrahost evolution of HIV-1 phenotypes
… R5 HIV-1 were more sensitivity to certain broadly neutralizing antibodies. Furthermore, phenotypic alterations correlated with the decline in CD4+ T cell count during development of immunodeficiency. The observed evolution in phenotypic features also correlated with molecular alterations of …
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The Ontogeny of Vaccine-Induced HIV-1 Glycan-Reactive Antibodies
… on the virion surface is the target for both non-neutralizing and neutralizing antibodies (nAbs). In both infection and vaccination, the dominant neutralizing antibody responses are strain-specific, however, during chronic infection, antibodies that can potently neutralize genetically diverse …
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