Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"broad substrate specificity"”.
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An investigation into the biocatalytic application of the thermostable nitrile hydratase from the thermophilic strain Geobacillus pallidus RAPc8
… with their physiochemical properties and broad substrate specificity. However, the widespread application of nitrile-converting enzymes in the industrial processes has been restricted in part by the thermal instability of the mesophilic-derived enzymes, and thus there is an increased focus …
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Protein and Pathway Engineering for Biosynthesis or Aromatic Compounds
… three molecules of malonyl-CoA, and exhibited broad substrate specificity, which was successfully altered via saturation mutagenesis guided by a homology structural model of PhlD. To improve phloroglucinol production for industrial manufacturing, a two-prone approach was undertaken. Firstly, …
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Maize β-glucosidase substrate specificity and natural substrates
… the plastid. Numerous glycosides were tested as substrates and K<sub>m</sub>'s and V<sub>m</sub>'s were determined. Various compounds were also tested as inhibitors and K<sub>i</sub>'s and/or K<sub>i</sub>’ 's were estimated. 4- methylumbelliferyl-β-D-glucoside was the best substrate …
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Autoregulatory and structural control of CaMKII substrate specificity
… and synaptic plasticity. CaMKII exhibits broad substrate specificity, targeting numerous substrates with diverse phosphorylation motifs. Binding of the calcium sensor CaM to the autoregulatory domain (ARD) of CaMKII functions to couple kinase activation with calcium signaling. Important …
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Using Dictyostelium discoideum to develop flavonoid therapeutics for polycystic kidney disease: the role of MATE transporters
… in unicellular and developing cells with broad substrate specificity, with increased transcription observed in the presence of all the flavonoids tested, and with ethidium bromide. Ddmate2 response was more complex, with differential increases in transcription to fewer flavonoids, within …
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Studies of the NAD metabolism of Haemophilus influenzae
… by EDTA. The enzyme was observed to have a broad substrate specificity and functioned in a manner indicative of negative cooperativity with all substrates except several modified in the adenine ring. The most effective inducer of negative cooperativity was NAD as indicated by its Hill …
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Molecular Cloning, In Vitro Expression, and Functional Characterization of an Ovine Gastrointestinal Peptide Transporter (oPepT1)
… of Na+, Cl-, and Ca2+. It displayed a broad substrate specificity that transported neutral and charged dipeptides and tripeptides. All dipeptides and tripeptides examined evoked inward currents in a saturable manner, with an affinity constant (Kt) ranging from 20 mM to .6 mM for …
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Characterization of Enzymes in the L-Asparagine Metabolism Pathway in Legumes
… are required to confirm this function. The broad substrate specificity of HPPR and HPR2 and its activity with 2-hydroxyacids indicate that its main role in vivo is to convert intermediary metabolites into metabolites that can be directly used for other important pathways within the plant. An …
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Cellular Uptake of Neutrohpil Elastase Links Inflammation to Adaptive Immunity
… cells leading to degradation of insulin receptor substrate-1 thereby promoting hyperactivity of the phosphatidylinositol-3 kinase (PI3K) pathway and tumor cell proliferation. To our knowledge, nobody has investigated uptake of NE by other tumor types. In addition, NE has broad substrate …
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Identification and characterization of the acid phosphatase from Francisella tularensis and its potential role in pathogenesis
… kDa, pH optimum of 6.0 for five different substrates, isoelectric point of 9.2, broad substrate specificity including. The enzyme was inhibited by molybdate, vanadate, and mercury but was resistant to scL-(+) tartrate, fluoride, chelators of divalent cations, and okadaic acid. FtACP …
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Omics Techniques Applied to Diabetes Research - Focus on HSL-Null Mice and Clonal β-Cells
… stored in adipose tissue. But HSL has broad substrate specificity and is widely expressed elsewhere than in adipose tissue. Its role in non-adipose tissues is not completely understood, but the phenotype revealed by the characterization of several independently generated HSL-null mouse …
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Development of peptide inhibitors as molecular therapeutics against tandem-repeat proteins
… (PARP) family of enzymes that PARylate their substrates, leading to ubiquitination and subsequent degradation. TNKS bind their protein substrates through an ankyrin-repeat domain comprising five so-called Ankyrin Repeat Clusters (ARCs). TNKS is of particular therapeutic interest in cancers due …
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Coarse-grained Modeling of Protein Dynamics using Elastic Network Models
… (APH), which confers resistance against a broad range of aminoglycoside antibiotics to pathogenic bacteria, drastically changes its flexibility upon binding of substrates, but without changing its average conformation. In contrast, the homotrimeric vesicular stomatitis virus glycoprotein G …
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Biosynthesis of polyhydroxyalkanoates for cardiac tissue engineering applications
… result. This result thus revealed the unusually broad substrate specificity of the PHA synthase from P. mendocina, which is able to catalyse both medium and short chain length PHAs biosynthesis depending on the metabolic pool available in the host organism. Sequence comparison of this PHA …
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Cloned xylanolytic and cellulolytic enzymes from an extreme thermophile
… protein concentration, were also important. The substrate specificities of the five enzymes were investigated. The β-xylosidase had a very narrow substrate specificity, with high activity only on o- or p-nitrophenyl β-D-xylopyranoside and no activity on xylobiose. It showed some transferase …
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Purification and characterisation of amylolytic enzymes from Lipomyces starkeyi
… Km and Vmax measurements using a range of substrates suggested that the aglucosidase had a broad substrate specificity but showed a marked preference for substrates with a-1,4 linkages such as soluble starch, PNPG, and a-1,3 linkages, such as n1gerose. SDS and native gel electrophoresis ( …
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The role of mitochondrial transporters in human physiology and adverse drug effects
… and to identify compounds that can inhibit substrate transport. So far, most drug interactions have been reported for the mitochondrial ADP/ATP carrier (AAC), therefore it was validated first as a model for studying drug-carrier interactions. For the first time, pure human ADP/ATP carrier 1 …
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Investigations into the Lipid Transport Mechanisms and Energetics of Bacterial ATP-Binding Cassette (ABC) Transporters MsbA and LmrA
… of the sodium-motive force in the transport of a broader range of substrates, this PhD study investigated the transport of long-acyl-chain (2 x C18) headgroup biotin-labelled phosphatidylethanolamine (PE) by LmrA. In addition, the potential involvement of polar and acidic residues within LmrA in …
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Angiotensin-converting enzyme cleavage of the Alzheimer's beta-amyloid peptide
… catalytic domains (N and C) with different substrate specificities. ACE is a central component of the intrinsic brain renin angiotensin-aldosterone system (BRAAS), well renowned as the regulator of blood pressure. The BRAAS has alternate functions that extend beyond fluid and blood pressure …
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