Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"brefeldin A"”.
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I Peptide isosteric replacement preparation using zinc-mediated chain extension-aldol reactions II Formal synthesis of (+)-Brefeldin A III Zinc-mediated chain extension reactions with substituted carbenoids
… key step. An asymmetric formal synthesis of (+)-Brefeldin A has been developed in which the zinc-mediated ring expansion/oxidation/elimination method constitutes a key step. Preparation of beta-alkyl-gamma-keto esters through a chain extension reaction using substituted carbenoids has also been …
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Strukturelle Untersuchungen zur Inhibierung und Biogenese des pflanzlichen Golgi-Apparates
… Im ersten Teil wurde die Wirkung der Hemmstoffe Brefeldin A und Nordihydroguairetischer Säure (NDGA) auf Sekretion und Struktur pflanzlicher Golgi-Apparate zu unterschiedlichen Zeitpunkten des Zellzykluses untersucht. Im zweiten Teil wurde die de novo Entstehung pflanzlicher Golgi-Apparate …
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The regulation of hypoxia-responsive gene expression by hydroxyl radicals and intracellular calcium
… Verschiedene Agentien darunter Tunicamycin, Brefeldin A und Thapsigargin wurden benutzt, um das ER in Stress zu versetzen. Dabei wurde gefunden, dass sowohl Tunicamycin als auch Brefeldin A die Hypoxie-induzierte HIF-1a Aktivierung aufheben. Thapsigargin, ein Hemmer der Ca2+-ATPase, hingegen …
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Migration of Nucleocapsids in Vesicular Stomatitis Virus-Infected Cells is Dependent on Microtubules and Actin Filaments
… of the glycoprotein of VSV in response to Brefeldin A (BFA) was used here to validate the BTBDM. This method was used to describe the distribution of the nucleocapsid of VSV during infection, and analyze the importance of the cytoskeleton on this distribution. To determine the mechanism by …
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Congenital Disorders of Glycosylation IIj (CDG-IIj): Identifizierung eines Defekts der COG6-Untereinheit des Conserved Oligomeric Golgi-Komplexes
… COG-Komplex abhängigen Proteintransportes nach Brefeldin A Behandlung, bewiesen durch Immunfluoreszenzanalysen.
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Identification and characterization of GRP50 : a novel Golgi-associated ankyrin repeat protein
… to the effects of the fungal metabolite, Brefeldin A. In biochemical fractionation experiments, GRP50 was purified from cultured cells as part of a large macromolecular complex. The possible roles of GRP50 in biological events at the cytoplasmic face of Golgi and vesicle membranes are …
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Evaluation of the role of autophagy in ovarian cancer chemoresistance
… autophagy was also evaluated using the inhibitor Brefeldin A. Autophagosome accumulation was reduced following treatment with Brefeldin A and following siRNA knockdown of BECN1 with the non-canonical regulator Rab9, which significantly reduced the recovery of OC cells following paclitaxel …
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Luminal calcium regulates membrane fusion in the early secretory pathway
… of the Golgi after it has been dispersed using brefeldin A. These results suggest that luminal calcium regulates fusogenicity, structure, and function of COPII derived membranes in transport. However, bulk soluble cargo is not affected by luminal calcium depletion. This suggests that luminal …
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Luminal calcium regulates membrane fusion in the early secretory pathway
… of the Golgi after it has been dispersed using brefeldin A. These results suggest that luminal calcium regulates fusogenicity, structure, and function of COPII derived membranes in transport. However, bulk soluble cargo is not affected by luminal calcium depletion. This suggests that luminal …
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CaV1.3 Ion channel expression and hormone therapy in prostate cancer
… following treatment with trafficking inhibitor, Brefeldin A and the histone deacetylase inhibitor, sodium butyrate. Overall, the results of this study show that CaV1.3 is relevant to AR-positive and androgen-sensitive PCa and is associated with neuroendocrine differentiation. ENZ treatment …
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Pathogen-induced protein secretion in plants.
… (SA). This SA-induced secretion is resistant to brefeldin A, an inhibitor of Golgi-mediated protein transport. Together with the absence of MTD in Golgi stacks and the lack of a documented extracellular targeting sequence in MTD, this suggests the secretion of MTD is by a non-Golgi, …
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Molecular Analysis of the Unconventional Export Machinery of Galectin-1, a beta-Galactoside-specific Lectin of the Extracellular Matrix
… die Sekretion von Galectin-1 in Gegenwart von Brefeldin A nicht gehemmt, so dass ein unkonventioneller Sekretionsweg postuliert wurde. In der vorliegenden Dissertation wurde ein robustes in vivo Modell zur funk¬tionellen Rekonstitution der Galectin-1 Sekretion etabliert. Es wurden mittels …
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Unconventional Secretory Pathways : Protein Folding and Quality Control During FGF2 Membrane Translocation
… auch nach Blockierung des ER/Golgi Systems durch Brefeldin A vollständig funktionell bleibt. Da FGF2 auch biomedizinisch ein sehr interessantes Targetprotein darstellt, ist die molekulare Aufklärung des Sekretionsweges von ausserordentlich grosser Bedeutung. In dieser Arbeit wurde ein …
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Characterization of a Novel GPI-anchored Protein, a Component of Sphingomyelin-enriched Microdomains at the Golgi Complex
… It is a Golgi resident protein and has a Brefeldin A-sensitive Golgi localization. Therefore, this protein was annotated GREG for Golgi-resident GPI-anchored protein. Mapping the Golgi-targeting signal in GREG shows a requirement of the EQ tandem repeat for its Golgi localization. …
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Turnover Rate of The Neuronal Connexin Cx36 In Hela Cells
… hours. Treatment of the pulse-labeled cells with Brefeldin A (BFA) prevented the addition of new connexins to junctional plaques, suggesting that the assembly of Cx36 into gap junctions involves the traditional ER-Golgi-TGN-plasma membrane pathway. In conclusion, Cx36-Halo is functional and has a …
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Secretion and delivery of virulence proteins from <i>Phytophthora infestans</i> to its host
… was inhibited in vitro and in planta by Brefeldin A (BFA), which is a conventional ER-to-Golgi-mediated secretion inhibitor. Wildtype cytoplasmic effectors follow an (BFA) insensitive secretion route for delivery into plant cells. <br/><br/>I hypothesised that extracellular vesicles (EVs) …
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Cellular Trafficking and Processing Pathways of the Alzheimer β/A4 Amyloid Precursor Protein
… functions. Treatment with either monensin or brefeldin A (BFA) prevented normal APP maturation (N- and O-glycosylation and tyrosine sulfation) and abolished APP secretion. Phorbol ester produced a several-fold increase in APP secretion, indicating that protein phosphorylation regulates …
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Characterization of the GBF1-Arf1 axis in enterovirus RNA replication
… organelles. The cellular protein Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 (GBF1) is essential for the replication of enteroviruses, but its molecular role in the replication process is unclear. In uninfected cells, GBF1 activates small GTPases of the Arf family …
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Modulation der Immunogenität von HPV-16 L1/E7 Chimären Virus-ähnlichen Partikeln
… of antigen presentation (TAP)-unabhängig, jedoch Brefeldin A-sensitiv war. CpG ODNs konnten die Immunogenität der CVLPs verbessern, allerdings nur dann, wenn sie vier Stunden nach CVLP-Beladung auf BMDCs geladen wurden. Die Sorbitol-Behandlung führte bei CVLP-beladenen BMDCs ebenfalls zu einer …
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Role of O-GlcNAc in the Vertebrate Secretory Pathway
… workflow we examined the effects of brefeldin A (BFA), a fungal metabolite that disrupts vesicle trafficking, and cytokine deprivation on a pro-B cell line. We identified changes in the O-GlcNAcylation of Coatomer subunit gamma-1 (COPG) a coat protein complex I (COPI) component in …
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