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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"bovine oocytes"”.
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Vitrification of Bovine Oocytes
Cryopreservation of oocytes is a challenge. Studies were conducted to vitrify mouse zygotes and cumulus-intact bovine oocytes from follicles of different diameters, small (≤ 4 mm) and medium (4 to 10 mm), using nylon mesh. The specific goals were to assess changes in apoptotic gene expression …
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EFFECT OF CRYOPROTECTANT EQUILIBRATION ON TRANSMEMBRANE POTENTIAL OF BOVINE OOCYTES
… perturbation on the transmembrane potential of bovine oocytes. The aim of this study was to evaluate and examine the difference in putative effects of non-permeating (PBS) and permeating (10% EG) solutions and to explore the association between osmotically induced volume changes on voltage in …
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Transcriptional profiles of cumulus-oocyte complexes related to developmental competence in bovine oocytes
During folliculogenesis, oocytes and cumulus cells undergo many morphological and physiological changes. Transcriptome data were produced from single oocytes and corresponding cumulus cells (CC) to infer the differences in the transcript abundance from fully grown versus growing phase oocytes and …
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Ultrasound-guided transvaginal follicular aspiration to provide a source of bovine oocytes for gene microinjection
… to determine an efficient method of obtaining oocytes from cows via ultrasound-guided transvaginal follicular aspiration. Transvaginal oocyte recovery combined with in vitro maturation, fertilization, and culture (IVMlIVFIIVC) can produce pre-implantation stage bovine embryos and also supply a …
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In-vitro developmental potential of bovine oocytes obtained by transvaginal follicular aspiration as related to their morphological quality and after microinjection of DNA
The development of oocytes of differing quality retrieved using transvaginal follicular aspiration (TVFA) and following DNA injection was examined. Eight cows were subjected to twice weekly TVF A for 16 wk. Oocytes retrieved were graded and placed in an in-vitro maturation, fertilization and …
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Biochemical and Physiological Factors Affecting Early Stages of Fertilization in the Bovine
… included in sperm binding to and penetration of bovine oocytes. The first part included evaluating conditions required to maximize sperm binding to and penetration in vitro; the second involved differentiating acrosin from non-acrosin proteases in bovine acrosomes. These proteases are likely to …
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SOMATIC SIGNALS REGULATING MRNA TRANSLATION IN MAMMALIAN OOCYTE.
In oocytes, resumption and completion of meiosis I are driven by post-transcriptional mechanisms on a background of repressed mRNA transcription. However, a comprehensive understanding of this regulation in higher-order mammals has been limited by challenges in isolating polysome-associated mRNAs. …
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Influence of Growth Factors on Bovine Embryo Development
… Cysteine supplementation to IVM media of bovine oocytes increases cellular glutathione production, which reduces reactive oxygen species (ROS). Similarly, beneficial effects of growth factors for improving the rate of blastocyst development have been reported, but combined effects are …
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Gene injection in the bovine: effect of time of microinjection and nuclear transfer technologies
… to investigate methods of producing transgenic bovine embryos entirely in vitro. Experiment 1 examined the effect of DNA microinjection at 11, 15 and 19 h after fertilization (haf) on survival rate and DNA detection frequency by polymerase chain reaction (PCR). There was no difference in …
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Immunoreactivity of anti pZP antibodies from the serum of SpayVac vaccinated mares to equine zona protein
… These techniques were first applied using a bovine model because bovine oocytes are more easily obtained in large quantities relative to equine oocytes. Once the procedure was validated, equine samples were utilized. Western blot analysis revealed immunoreactivity of anti pZP antibodies that …
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Post-Transfer Outcomes in Cultured Bovine Embryos Supplemented with Epidermal Growth Factor, Fibroblast Growth Factor 2, and Insulin-Like Growth Factor 1
… industry. Use of in vitro fertilized (IVF) bovine embryos has become increasingly popular to help alleviate several of these reproductive issues and provide a means to enhance genetic gain for production traits. An uterine paracrine factor cocktail containing epidermal growth factor (EGF), …
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DISSECTING THE ROLE OF REACTIVE OXYGEN SPECIES IN EARLY MAMMALIAN EMBRYOGENESIS
… matrix, which was microinjected into immature oocytes (GV) and in vitro matured oocytes (MII). These microinjected oocytes were then matured or fertilized in vitro. To assess the sensor’s ability to detect H2O2 with high spatial and temporal resolution, samples were imaged under control …