Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 27 for “"bovine embryos"”.
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Embryo-maternal interactions during the early development of bovine embryos
La comunicación recíproca entre el embrión y el endometrio es esencial para la implantación embrionaria y la preñez a término en mamíferos. La ausencia de factores involucrados en esta comunicación durante el cultivo in vitro merma la eficiencia de las técnicas de reproducción asistida. Esta tesis …
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Effects of culture conditions on development of early murine and bovine embryos
Early mouse (n = 501) and bovine (d = 6; n = 172) embryos were obtained to evaluate the effect of a deproteinized hemodialysate (CLBl 107) on embryo development in vitro. Bovine morulae also were cultured to examine the effect of agar embedding and the environment of immature mouse uteri on embryo …
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Application of insect freeze tolerant strategies to the freezing of bovine embryos
… (10% v/v) level on the survival of frozen-thawed bovine embryos. In Exp. l, survival rates of 6 and 7 day bovine embryos frozen in medium containing .1% hemolymph and seeded at -5 C or -7 C, were compared to evaluate the effect of seeding temperature. The effect of hemolymph was evaluated by …
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Energy substrates, metabolic regulators, and lipid accumulation during culture of in vitro-produced bovine embryos
… was to optimize in vitro culture conditions for bovine embryonic development, using alternative energy sources and metabolic regulators. Replacing glucose with fructose in culture medium consistently increased blastocyst production per oocyte and decreased lipid content in bovine embryos. The use …
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Interleukin-6 Supplementation Improves Post-Transfer Embryonic and Early Fetal Development of in vitro Produced Bovine Embryos
In vitro produced (IVP) bovine embryos are useful for investigating the mechanisms affecting early embryonic failure. The work in this thesis explored how interleukin-6 (IL6), an embryokine that increases inner cell mass (ICM) influences post-transfer embryonic survival and development of the …
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Supplementing Bovine Embryo Culture Media to Improve the Production and Quality of In Vitro Produced Bovine Embryos
… inhibitory factor (LIF) in preimplantation bovine embryos. Neither cytokine affected the total percentage of embryos which developed to the blastocyst stage in vitro. However, supplementation of IL6 increased blastocyst inner cell mass (ICM) cell number without affecting trophectoderm (TE) …
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Effects of Differences in Dietary Protein and Varying the Interval from Collection of Bovine Embryos to Freezing on Embryo Quality and Viability
… dietary protein on the production and quality of bovine embryos collected from superovulated donors. Angus cows were randomly assigned to receive one of three experimental diets: a daily ration of 5.7 kg poultry litter, 2.0 kg hay, 3.1 kg corn, and 0.5 kg peanut hulls (LITTER; n = 15); a daily …
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Post-Transfer Outcomes in Cultured Bovine Embryos Supplemented with Epidermal Growth Factor, Fibroblast Growth Factor 2, and Insulin-Like Growth Factor 1
… industry. Use of in vitro fertilized (IVF) bovine embryos has become increasingly popular to help alleviate several of these reproductive issues and provide a means to enhance genetic gain for production traits. An uterine paracrine factor cocktail containing epidermal growth factor (EGF), …
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The chick embryo amnion as an in vitro culture system for IVF and NT embryos
… reduced following in vitro production of embryos. Thus, if a technique could be developed that would increase calving rates by as little as one viable offspring, significant research advances could be made. Therefore, in a series of experiments, the efficiency and quality of culturing IVP …
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Gene injection in the bovine: effect of time of microinjection and nuclear transfer technologies
… to investigate methods of producing transgenic bovine embryos entirely in vitro. Experiment 1 examined the effect of DNA microinjection at 11, 15 and 19 h after fertilization (haf) on survival rate and DNA detection frequency by polymerase chain reaction (PCR). There was no difference in …
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Bovine embryo microinjection, culture, microsurgery, and DNA analysis by the polymerase chain reaction technique
… the optimum in vitro culture system for one-cell bovine embryos. Subsequent experiments compared bisection and biopsy for acquisition of cellular material from bovine morulae for DNA amplification by the polymerase chain reaction technique (PCR), and evaluated the use of DNA microinjection, in …
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The Role of Amino Acids and the Threonine-SAM Pathway in the Development of Bovine Inner Cell Mass and Pluripotency
… cell mass (ICM) of preimplantation mouse and rat embryos, known as embryonic pluripotent stem cells (ePSCs) or embryonic stem cells (ESCs) or via reprogramming of somatic cells to their pluripotent state, known as induced pluripotent stem cells (iPSCs). PSCs are excellent tools for gene editing …
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Characterization of Maternal Factor MOF/H4K16ac and the Impact of the Cryostress on Bovine Embryonic Development
… is critical for mammalian embryo viability. Bovine embryos, which share high transcriptomic similarity with humans, serve as valuable models for understanding fundamental biological processes. Insights gained from studying bovine embryogenesis can transduce to broader biological research and …
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Supplementing IL6, IL11, and LIF to Improve Cultured Bovine Oocyte Competency
Bovine embryos produced in vitro consistently display decreased quality in terms of their potential to reach the blastocyst stage as well as post-transfer survival. Media formulations, oocyte quality, and inferior expression of needed transcripts are all causes of this reduced developmental …
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Development of an Interferon Bioassay and Primitive Endoderm Cell Lines to Study Lineage Specification During Early Bovine Embryogenesis
… the development of a PrE cell line derived from bovine embryos. The PrE outgrowths can be produced at high rate, and can be maintained in a continuous culture system for about 6 weeks. As a true bovine PrE cell line does not currently exist, these lines hold great potential for the study of early …
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Maturation and Culture Media Effects on In Vitro Bovine Embryo Developmental Competence
<p><em>In vitro</em> produced bovine embryos are critical to the cattle industry. However, these embryos have altered morphology, epigenetics, and metabolism when compared to their <em>in vivo </em>counterparts. The aim of this thesis was to alter maturation and culture media to improve the …
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Developing embryo technologies for the eland antelope (Taurotragus oryx)
… on the in vitro development of in vitro-derived bovine embryos were evaluated. A 2 x 2 factorial study comparing effects of groups of amino acids (20aa or 11aa) in two base media (modified KSOM or BM-3) demonstrated that amino acids and base medium affected embryonic development. A subsequent 7 x …
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Ultrasound-guided transvaginal follicular aspiration to provide a source of bovine oocytes for gene microinjection
… (IVMlIVFIIVC) can produce pre-implantation stage bovine embryos and also supply a source of ova for gene microinjection. In Experiment 1, once- (IX) vs. twice-weekly (2X) oocyte recovery frequencies were compared. No differences in recovery per session were observed (IX = 6.8 vs. 2X = 6.3 …
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Superfetation in beef cattle
… (AI) of pregnant cows and (2) transfer of 7-day embryos to cows with more advanced pregnancies. An attempt to produce superfetation by induction of ovulation in mated cattle with human chorionic gonadotropin (hCG) followed by AI was unsuccessful. Ovulation was induced in pregnant cows on day 7 …
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