Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"bovine aortic endothelial cells"”.
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Purification and Characterization of Proteoglycan from Bovine Aortic Endothelial Cells Conditioned Media, and its Interaction with Basic Fibroblast Growth Factor (bFGF)
Cultured bovine aortic endothelial (BAE) cells were found to synthesize and secrete heparan sulfate proteoglycans (HSPG), which bound basic fibrobalst growth factor (bFGF). bFGF is a known mitogen for vascular smooth muscle cells, and is indicated to have a role in some proliferative vascular …
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Vascular endothelial cell-surface proteoglycans
… from cell layers of normal ("cobblestone") bovine vascular endothelial cells in culture. Treatment of the parent molecules with dithiothreitol caused their complete cleavage and permitted the subsequent separation of the larger and smaller subunits on Sepharose CL4B columns. Removal of …
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Design of a microreactor for studying the effect of shear stress on angiogenesis in self-assembling peptide matrix
… substrate, a self-assembling peptide gel, and bovine aortic endothelial cells. A theoretical model was developed to approximate the permeability of the peptide gel and to quantify the average shear stress on an endothelial cell seeded in a 3D matrix of the peptide gel. Experimental results in a …
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In Vitro Binding and Transport Regulation by Endothelial Cells: Preliminary Studies looking at FIX and IGF-I
Endothelial cells separate the bloodstream from the underlying tissue and play a crucial role in vascular homeostasis. They also form an important barrier for vascular drug delivery. This thesis contains preliminary studies targeted at understanding the mechanisms of binding and transport across …
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Kinetics study of heat shock protein 70 expression
… discover endogenous HSP70 expression kinetics. Bovine aortic endothelial cells (BAEC) were heated in a 42oC incubator for up to 5 hours and subsequently held at 37oC for up to 48 hours. Western blot and quantitative protein analysis were performed to measure the extent of HSP70 expression …
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A study on the resistances to oxygen transport in the microcirculation
… this work, the oxygen permeability of cultured bovine aortic and human umbilical vein endothelial cell monolayers was measured directly in vitro. The monolayers were cultured on silicone rubber membranes and placed in a carefully calibrated diffusion cell immersed in a 37$\sp\circ$C waterbath. …
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An Intramural, Tension-modulating Reflex in the Rat Caudal Artery
… either neuronal, vascular smooth muscle, and/or endothelial origins. We have shown that EFS-induced relaxation of intact, phenylephrine (PE) preconstricted rat tail artery (RTA) rings was dependent upon the extracellular concentration of Ca++. Inhibiting either nitric oxide (NO) synthesis with …
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Effect of the Insulin-like Growth Factor (IGF) Axis on the Transport Properties of Endothelial and Epithelial Cells In Vitro
… first objective, parental and IGF-I secreting bovine mammary epithelial cells were tested for cell layer permeability, tight and adherens junction proteins, IGF-IR, and a downstream signaling components of IGF-IR. In comparison with parental cells, IGF-I secreting cells had high levels of …
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Chronic Shear Stress Effects on Endothelial Cell Response
… secretion of important regulatory molecules by endothelial cells. Our hypothesis was that inclusion of chronic pulsatile shear stress in our model would lead to changes in endothelial cell release of regulatory molecules. We distinguished between high arterial shear stresses and low venous shear …
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CAVEOLAE AS SPATIO-TEMPORAL COMPARTMENTS FOR ROS/RNS GENERATION AND NITROXIDATIVE STRESS SIGNALING
… oxidase enzyme complexes and eNOS localize to endothelial caveolae microdomains. Additionally, caveolae internalization has been shown as an activating mechanism for enzyme eNOS. However, roles of caveolae in ROS/RNS generation and downstream signaling roles in activating endothelial cells are …
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Lipoprotein receptors in cultured bovine endothelial cells
Endothelial cells take up and degrade both low density lipoproteins and low density lipoproteins which have been modified by acetylation (AcLDL). In this study, receptors that may be involved in the uptake of these lipoproteins were characterized. The cells used were aortic endothelial cells …
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Pharmacological and Molecular Characterisation of P2Y Receptors in Endothelial and Epithelial Cells
… this study were to characterise epithelial and endothelial cells for a thorough expression profile of P2Y receptor mRNA transcripts in order to provide a rapid screen for the molecular determinants of these receptors in these cells. These studies also aimed to confirm previously published …
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The role of death-associated protein kinase in endothelial apoptosis under fluid shear stress
<p>Endothelial cells are the interface between hemodynamic fluid flow and vascular tissue contact. They actively translate physical and chemical stimuli into intracellular signaling cascades which in turn regulate cell function, and endothelial dysfunction leads to inflammation and diseased …
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Receptor-mediated endocytosis of low density lipoproteins in aortic endothelial cells
… (subconfluent) and quiescent (postconfluent) bovine aortic endothelial cells (ECs) were qualitatively investigated by fluorescence microscopy using dioctadecylindocarbocyanine-labelled lipoproteins and by indirect immunofluorescence microscopy. LDL and acetylated-LDL (AcLDL) were seen bound to …