Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 67 for “"bone marrow-derived macrophages"”.
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Novel Characteristics of Murine Bone Marrow-Derived Macrophages and Human Macrophage-Like Cells
… studies provide evidence for novel properties of macrophages derived from bone marrow stem cells. In study 1, treatment of activated mouse bone marrow-derived macrophages (BMM) with either catecholamine synthesis inhibitors (α-methyl-para-tyrosine and fusaric acid) or the β2 adrenergic receptor …
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Optimization and Validation of the Derivation and Use of HoxB8 Transgenic Murine Phagocytes in In Vitro Studies
… on the microenvironment within phagosomes in macrophages. The research in this area is limited due to the use of bone marrow-derived macrophages that are short-lived, require the constant sacrifice of mice, and are very difficult to genetically manipulate. To overcome these shortcomings, we …
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Characterisation of a novel leukocyte receptor complex-encoded receptor TARM1
… and immature CD11b+Gr+ neutrophils isolated from bone marrow but not at peripheral sites. Following ip LPS treatment or systemic bacterial challenge, TARM1 protein expression was upregulated by myelocytes, mature neutrophils and inflammatory monocytes and TARM1+ cells were rapidly recruited to …
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Transcriptional Regulation of NLRC4 Inflammasome by IRF8
… We found that NLRC4 inflammasome activation in bone marrow-derived macrophages (BMDMs) is greatly dependent on interferon regulatory factor 8 (IRF8). NLRC4-mediated caspase-1 activation and subsequent production of the inflammasome-dependent cytokines IL-1β and IL-18 and cell death were impaired …
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Studies using luciferase-expressing Leishmania donovani parasites
… miltefosine and paromomycin. Intraperitoneal or bone marrow derived macrophages infected with L. donovani were incubated with different concentrations of each drug (sodium stibogluconate, 4-0.02mg/ml; amphotericin B, 4-0.02μg/ml: miltefosine, 4-0.02μg/ml; paromomycin, 4-0.02μg/ml) and the amount …
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Modulation of macrophage metabolism and function by the helminth peptide FhHDM-1
… metabolism. Specifically, FhHDM-1 switched macrophages to a dependence on oxidative phosphorylation fuelled by fatty acids and supported by the induction of glutaminolysis. These changes mediated a decrease in glycolytic flux which consequently reduced pro-inflammatory cytokine production. …
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Manipulating macrophages to enhance liver regeneration
… therapy for life-threatening cases. Hepatic macrophages are crucial for innate immune integrity and effective hepatocyte proliferation. The macrophage may therefore present a novel therapeutic target to enhance regeneration following acute liver injury. In this thesis I describe the …
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The Hypoxic Adenosine Response Modulates Macrophage Differentiation and Contributes to Lung Disease
… Additionally, alternatively activated alveolar macrophages (AAMs) expressing ADORA2B, have been implicated in mediating adenosine’s pro-fibrotic effects in IPF. However, the exact role of AAMs in the hypoxic lungs of IPF patients is not known. Our results reveal myeloid-specific ADORA2B …
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Molecular Mechanisms Involved in Interleukin-1β Release by Macrophages Exposed to Metal Ions from Implantable Biomaterials
… longevity. Previous studies have shown that, in macrophages, Co2+, Cr3+, and Ni2+ can activate the NLR family pyrin domain-containing protein 3 (NLPR3) inflammasome, which is responsible for interleukin(IL)-1β production through caspase-1. Furthermore, these ions are known to induce oxidative …
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Sensing of Picornavirus Infections
… Picornaviridae family were investigated. Using bone marrow derived macrophages from MDA-5 and RIG-I null mice we showed that encephalomyocarditis virus (EMCV) and coxsackievirus B3 (CVB3), picornaviruses of the cardiovirus and the enterovirus genus respectively, are sensed by both MDA-5 and …
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The role of PLK1 in NLRP3 inflammasome activation
… are mutually exclusive events in murine BMDMs (Bone Marrow Derived Macrophages) and that PLK1 is an important regulator of NLRP3 inflammasome activation. Using two different potent and specific PLK1 inhibitors, I demonstrated that PLK1 inhibition impairs the NLRP3 inflammasome pathway. …
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Enhancing Anti-Cancer Immunotherapy by Disruption of the non-Receptor Tyrosine Kinase Fes
… kinase, which is abundantly expressed in macrophages, dendritic cells, NK cells and B cells, as an innate intracellular immune checkpoint. In syngeneic engraftment models of breast cancer and melanoma, FES genetic disruption in the host was associated with delayed tumour growth, improved …
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Regulating Inflammation Through Exercise-Mediated Trained Immunity or Genetic Manipulation of the Interferon Locus
… exercise training could induce adaptations in bone marrow-derived macrophages, resulting in trained immunity, known to occur in immune cells due to persistent metabolic and epigenetic changes. Data from this study suggest that chronic moderate exercise can influence the inflammatory responses …
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Investigating the Reconstitution and Function of Peritoneal Cavity Macrophages after LPS-induced Peritonitis
The tissue-resident macrophages of the peritoneum are sentinel immune cells, in constant motion within the serous fluid of the abdomen. Like most tissue macrophages, the GATA6+ large peritoneal macrophages (LPMs) are responsible for the clearance and removal of pathogens that may break through the …
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Functions of Phosphatidylinositol 4-Phosphate 5 Kinases in Actin Cytoskeletal Regulation During Phagocytosis
… synthesize PIP2, during phagocytosis. PIP5Kg-/- bone marrow-derived macrophages (BMM) have a highly polymerized actin cytoskeleton and are defective in attachment to IgG-opsonized particles and FcgR clustering. Delivery of exogenous PIP2 rescued these defects. PIP5Kg knockout BMM also have more …
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Colony Stimulating Factor-1 Receptor (CSF-1R) Expression, Degradation, and Signaling in Lipid-Laden Macrophages
… of the world’s population. In atherosclerosis, macrophages become filled with lipid droplets and form fatty plaques in artery walls. The formation of these atherosclerotic plaques is dependent on the macrophage growth factor colony stimulating factor-1 (CSF-1); however, understanding the changes …
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Characterization of Regulatory Mechanisms in Mucosal Immunity by Systems Immunology
… subset of IL10-producing tissue resident macrophages. Secondly, we employed an ex vivo H. pylori co-culture with bone marrow derived macrophages, that together with a global transcriptomic analysis and a bioinformatics pipeline, lead to the discovery of promising regulatory genes based on …
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Caracterização da resposta imunometabólica frente a infecção por Leishmania amazonensis e Leishmania braziliensis em macrófagos de camundongos BALB/C
… cells of the innate immune system, particularly macrophages, and possess the ability to modulate cellular metabolism during infection, favoring their survival. The present study investigates the metabolic alterations induced by Leishmania spp. in the energy metabolism of macrophages, based on the …
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The role of the inositol phosphatase, SHIP, in the innate immune response to Salmonella Typhimurium
… and analysis of supernatants taken from M2 bone marrow derived macrophages correlated with this data. M2 macrophages were the predominant population in vivo during both oral and intraperitoneal infections. Because M2 macrophages are poor defenders against bacterial infection, these data …
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