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Showing 1 to 14 of 14 for “"bo3"”.
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The interactions of cytochrome bo3 from Escherichia coli with its substrates - ubiquinone and oxygen
… c. Escherichia coli (E. coli) cytochrome bo3 is the best characterized quinol oxidase. Depending on the detergent used to solubilize the enzyme, cyt bo3, preparations of this enzyme contain between 0 to 2 equivalents of ubiquinone-8. Studies using ubiquinol-1, a soluble quinol substrate, …
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EPR and solid-state NMR studies on the mechanism of cytochrome bo3 ubiquinol oxidase from escherichia coli
Cytochrome bo3 ubiquinol oxidase from E. coli is a member of heme-copper oxidase superfamily. This trans-membrane enzyme complex catalyzes two-electron oxidation of ubiquinol and reduction of molecular oxygen to water. During the process, the protons from ubiquinol are released to the periplasmic …
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Structural and Functional Studies on Cytochrome BO3 Ubiquinol Oxidase From Escherichia Coli and the Characterization of Its Quinone Binding Sites
Cytochrome bo3 ubiquinol oxidase is the terminal oxidase in the aerobic respiratory chain of Escherichia coli. The enzyme catalyzes the oxidation of ubiquinol-8 and the reduction of oxygen to water, which are coupled to the translocation of protons across the cytoplasmic membrane via protolytic …
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Low-dimensional and frustrated magnetism in complex lanthanide oxides
… First, the lanthanide calcium oxyborates Ca4LnO(BO3)3, Ln = Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Yb, were synthesised using solid-state ceramic methods. In Ca4LnO(BO3)3 the magnetic Ln3+ ions form quasi-one-dimensional chains. The crystal structure was investigated using powder X-ray and neutron …
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Biochemical characterization of a-type heme-copper oxidases in escherichia coli, bacillus subtilis and thermus thermophilus
… at quinone binding site in E. coli cytochrome bo3 oxidase. Similar result was reported on cytochrome aa3-600 oxidase in B. subtilis, a close homolog to cytochrome bo3 that uses menaquinone instead of ubiquinone. Recently, in vivo experiments have demonstrated that cytochrome bo3 could also use …
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Studies on the structure, function and mechanisms of the cbb3 type cytochrome c oxidase from Vibrio cholerae and the cytochrome bo3 ubiquinol oxidase from Escherichia coli
… type cytochrome c oxidase from V. cholerae and bo3 type ubiquinol oxidase from E. coli were investigated separately. Site directed mutagenesis were carried out in the case of cbb3 protein based on crystal structure and sequence alignment. Activity data suggested that the D pathway analogue is …
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Structure and Function Relationships in Cytochrome Bo(3) Oxidase and Cytochrome Bd-I Oxidase From Escherichia Coli
… coli have been investigated. Cytochrome bo3 oxidase, a member of the heme/copper superfamily, is found to have a semiquinone intermediate during turnover of quinol. Additionally, after the quinol is consumed and turnover stops, the enzyme is found in a mixture of oxidation states, one of …
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Exploring the Structure-Function Relationships and Dynamics of the Heme/copper Oxidase Superfamily Using Cytochrome Bo(3) From Escherichia Coli as a Model System
… are shown, which demonstrate that cytochrome bo3 can be constituted into stable thin films, suitable for ATR-FTIR work.
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Examining Molecular Interactions of Proteins by Isotopic Labeling, Sample Formulation and Solid-State NMR Spectroscopy
… two large membrane proteins, E. coli cytochrome bo3 oxidase and A. thaliana cytochrome P450 monooxygenase 98A3. To obtain site-specific resolution in uniformly- 13C, 15N labeled samples of these proteins, hardware advances, new experimental techniques and increased dimensionality were combined. …
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Borate polyanion-based systems as Li- and Mg-ion cathode materials
… pyroborates, M2B2O5, and orthoborates, M3(BO3)2, where M = Mg, Mn, Co, Ni, as high capacity and high voltage Li- and Mg-ion cathode materials. We explore the layered orthoborates (M3(BO3)2 which, to our knowledge, have not been previously considered as Li- or Mg-ion cathodes, perhaps due to …
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Characterizations on bacterial photosynthesis and respiration with optical spectroscopy and magnetic resonance
… section, the semiquinone pocket in the bo3 oxidase from Escherichia coli is examined with pulsed EPR (Chapter 5). To get a clear picture of the hydrogen bonded protons, I have fully deuterated the enzyme and successfully generated the semiquinone signal, with which the Davies ENDOR are …
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Entschlüsselung des Genoms von Gluconobacter oxydans 621H - einem Bakterium von industriellem Interesse
… von G. oxydans beinhaltet zwei Chinol Oxidasen (bo3 und bd) und eine nicht-protonentranslozierende NADH-Dehydrogenase. Die Energiegewinnung erfolgt durch membrangebundene Dehydrogenierungsreaktionen.
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Solid-state NMR studies of membrane proteins and membrane protein complexes
… are used to study a 144 kDa cytochrome bo3 ubiquinol oxidase demonstrating the power of this technique to investigate large membrane complexes in native environments.
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New Developments in the Crystal Chemistry of Selected Borophosphates and Phosphates
… built of interconnected trigonal–planar BO3 and / or BO4 and PO4 groups and their partially protonated species. The main objective of the present work was to synthesize, characterize and to study the properties of new selected 3d transition metal borophosphates. The selected four elements …