Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 509 for “"blotting"”.
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Diagnosis of Bovine Anaplasmosis Using Anaplasma Antigenic Markers Identified by Western Blotting
… of using antigenic markers detected by Western blotting (WB) for diagnosis of bovine anaplasmosis was studied.
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Creating a Map for Surgeons: Direct Blotting Assisted Stamping of Tissue for MALDI Imaging Mass Spectrometry
MALDI imaging mass spectrometry is an attractive technique for the discovery of disease biomarkers from surgically removed tissue sections. It can provide spatial resolution and relative abundance of a wide range of biomolecules across the tissue surface. While the technology has advanced …
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Regeneration, DNA Methylation, and Transformation in Rosaceae
… of Ace-AMP1 and npt II genes by PCR, Southern blotting, and Northern blotting. Transgenic rose lines inoculated with conidial spores of Sphaerotheca pannosa (Wallr.: Fr.) Lev. var. rosae showed enhanced resistance to powdery mildew.
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Towards Gene Therapy of Parkinson’s Disease: Implantation of RUC-GDNF Secreting Mammalian Cell Lines into Rat Brain
… conventional methods such as ELISA and Western blotting, animals must be sacrificed to retrieve implanted cells in order to study their viability and functionality. Real-time monitoring of GDNF production by implanted cells in live animals was not possible.</p> <p>To overcome the limits, a cell …
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The anti-cancer activity of extracts derived from millets and Kraalbos
… cells. Scratch motility assays and western blotting with antibodies to epithelial to mesenchymal transition markers showed that KB2 inhibited the migratory ability of breast cancer cells. To understand the mechanism(s) underpinning the anti-cancer activity of KB2, ROS-GloTM H2O2 and …
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Characterisation and secretion mechanism of Bordetella pertussis autotransporter proteins
… of Bap-5 was confirmed by RT-PCR and Western blotting and was shown to be bvg dependent. Although Bap-5 does not possess a typical signal sequence like pertactin (PRN), its surface localisation was confirmed by agglutination and immunofluorescence assays. A potential role for Bap-5 in …
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Molecular analysis of the dry rot fungus Serpula lacrymans
SDS-PAGE/silver staining and western blotting were used to investigate the molecular profile of a PBS soluble extract from standard mycelium profile of S. lacrymans FPRL 12C. Both techniques enabled a reference profile for this organism to be established. A polyclonal antiserum raised against …
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Investigating the potential of the Metastasis Associated Antigen 1 (MTA1) for cancer immunotherapy in a murine model
… protein levels were also confirmed by western blotting. It was observed that most of the cell lines expressed MTA1 at high levels, whereas no protein expression was detected in the normal tissues by western blotting. Next, we decided to identify MHC class I and II restricted immunogenic …
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The cardioprotective mechanisms of dietary flavonoids
… MAPK phosphorylation was monitored using western blotting. It was shown that quercetin was the most potent flavonoid at inducing a protective effect, and 3’-O-methylquercetin the most potent metabolite. Using western blotting it was shown that this protective effect is most likely due to …
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Characterization of Chitinase Activity and Gene Expression in Muskmelon Seeds
… because they possess signal peptides. Southern blotting suggested that there is at least one additional gene similar to Cmchi1 in the muskmelon seed genome, while there is only one copy of Cmchi2. Northern blotting analysis showed that both Cmchi1 and Cmchi2 are expressed in the radicle tissue …
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Cytological and Biochemical Studies of Male Sterility in Two Species of Drosophila
… isoelectric focusing and Western blotting to determine the biochemical basis for sterility. One protein, KRP60, was consistently reduced in sterile males. This protein may play a role in the normal function of the sperm in early development.
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The proteomic analysis of resistance to anticancer therapy in human breast cancer
… in resistance to anticancer therapy. Western blotting was used to confirm the expression of selected targets and to ensure the accuracy of protein identification. Examples of those targets which were confirmed by western blotting include GST-M3, cytokeratin 17, peroxiredoxin 4 and HSP27 (which …
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Expression and Clinical Significance of the N-myc downstream regulated gene-1 in Hypopharyngeal Cancer
… polymerase chain reaction (RT-PCR) and Western blotting, fresh normal and cancer tissue from the hypopharynx obtained from three patients were used. Results. Positive rates of Ndrg-1 expression in hypopharyngeal SCC were 21.4%, respectively. Ndrg-1 staining was significantly inverse correlated …
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Variation and Divergence of Repetitive DNA Sequences With Partial Identity to the Knob Sequence in Tripsacum and Zea
… in the genome as ascertained by southern blotting. Phylogenetic variation of these sequences indicates that although major features of these repeats have been conserved throughout Tripsacum and Zea, subsets of these repeats have gone through various amplification cycles.
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The Role of RNAi in Mammalian Cells in Response to Sindbis virus infection
… svRNAs, these were undetectable using northern blotting. We have also found that the expression profile for cellular miRNAs did not change in the early stages of virus infection according to the sequencing data, a finding which was verified by northern blotting. A functional RNAi assay was …
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MicroRNA as Regulator of the Th17 Inflammation in Behcet's disease
… T cells. Luciferase reporter assay and western blotting were carried out to determine whether Ets-1 is a target of miR-155. Subsequently, Ets-1 expression was quantified using real-time PCR in patients and controls. Western blotting was also performed to investigate the relationship between …
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Roles of Interferon Lambda on Hepatitis B Virus Replication
… cells with IFN-λ1 by real-time PCR and Southern blotting. After treatment of PEB8 with IFN-λ1, I examined the transcription level of HBV by Northern blotting and the amount of secretory viral Ag by ELISA. I performed the RT-PCR to investigate the induction of antiviral proteins, MxA and 2’5’-OAS, …
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The Possible Role for Basigin Receptor Function in The Induction of Matrix Metalloproteinase Expression in Human Endometrial Stromal Cells
… screened for the knock-out using PCR and western blotting. I determined that the donor plasmid was inserted into the genomes, but not in the intended location. Upon western blotting, all clones contained basigin protein expression. This could be due to basigin being a key gene in cell regulatory …
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Isolation and characterization of bacterial polyhydroxybutyrate inclusions
… were characterized using 13C NMR, western blotting, SDS-PAGE, atomic force microscopy, and fluorescence microscopy. A model for the structure of native PHB inclusions is proposed.
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