Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 133 for “"blastocyst"”.
-
Automation of Single Cell Micromanipulation and Microsurgery for Blastocyst Embryo Biopsy
… presents novel methods for the automation of the blastocyst embryo biopsy steps, using the conventional tools currently available in the research labs and the in vitro fertilization clinics (IVF), computer vision, and image based control algorithms. This thesis includes four main chapters, each …
-
Investigating the Actions of Interleukin-6 Family Members on Post-Hatching Blastocyst Development in Cattle
… post-hatching in vitro produced (IVP) bovine blastocyst development. The first series of studies determined if adding human recombinant IL6 improved IVP blastocyst quality before and after cryopreservation. Adding IL6 at 25, 50, and 100 ng/ml increased inner cell mass (ICM) cell number but did …
-
ROLES OF HAND2 TRANSCRIPTION FACTOR IN UTERINE RECEPTIVITY AND DECIDUALIZATION
Blastocyst implantation is the process in which a competent blastocyst acquires the ability to tether into the mother endometrium. At the same time, the endometrial tissue undergoes the process of decidualization to support the anchoring of the blastocyst and provides the blastocyst with nutrition …
-
Optimizing embryo culture conditions and spent culture media analysis as predictors of embryo quality and pregnancy
… incubators. Single-step media resulted in better blastocyst development compared to sequential media and the EmbryoScope™ incubation system showed slight improvements in embryo development than the Planer system. The benefits of supplementing the culture medium with either insulin or insulin-like …
-
Effect of different culture media and incubation methods on culturing murine embryos in vitro using a semen straw as an alternative receptacle
… in TCM-199 and Ham's F10 culture media until the blastocyst formation. The spermatozoa and oocytes of an F1 generation (Balb C x C57) were fertilized in Ham's F10 and TCM-199 in semen straws and in micro-drops in petri dishes. The embryos were cultured in TCM-199 and Ham's F10 in semen straws and …
-
Supplementing Bovine Embryo Culture Media to Improve the Production and Quality of In Vitro Produced Bovine Embryos
… percentage of embryos which developed to the blastocyst stage in vitro. However, supplementation of IL6 increased blastocyst inner cell mass (ICM) cell number without affecting trophectoderm (TE) cell number. Additionally, we found that IL6 activated signal transducer and activator of …
-
Telomerase Activity in Human Preimplantation Embryos
… activity levels for the oocyte through blastocyst, and finally, evaluate the use of measuring telomerase activity within biopsied blastomeres to predict blastocyst development. Telomerase positive (DU145 and PMEF) and negative (Hs27 and Detroit 551) cell lines were used to evaluate the …
-
Evaluation of techniques for the production of transgenic animals
… 44% of 4- cell, 40% of morula, and 29% of blastocysts. By comparison, the integration frequency for transgenic mice made using the same DNA construct was 22%. After 5 days of in vitro culture, the injected construct was detected in 83% of arrested 1-cell, 85% of arrested 2-cell, and 85% of …
-
Cell Fate Decisions in the Early Mammalian Embryo
… of a tri-lineage structure known as the blastocyst, consisting of the epiblast, primitive endoderm and trophectoderm, which will give rise to the new organism, the yolk sac and placenta respectively. These three lineages must be established from the totipotent zygote via two successive …
-
DNA methylation in the early porcine embryo
… of the donor cells to direct development to the blastocyst stage was analyzed. The CpG methylation remodeling that occurs in the development of the in vivo derived blastocyst does not occur in blastocysts produced by using in vitro techniques such as parthenogenesis, NT, and in vitro …
-
The effect of uterine flushings on early bovine embryo development in vitro
… .01%). Embryos were cultured up to the hatched blastocyst stage or degeneration and evaluated at 12 h intervals. Treatment differences were evaluated by analysis of variance after assigning a value of 0 to 5 to each embryo representing its final stage of development. Overall analysis revealed …
-
Oxidative stress mechanisms within the developing porcine oocyte and the effects of antioxidant supplementation
… GSH, nuclear maturation or fertilization traits. Blastocyst formation for NAC (35.0 ± 7.4%) and NACA (40.0 ± 7.4%) supplementation were higher (P < 0.05) than the control (20.0 ± 7.4%) and GSH supplemented (20 ± 7.4%) oocytes. The same pattern was seen for ICSI-derived embryos: blastocyst …
-
IN VITRO EMBRYO PRODUCTION IN WOOD BISON (Bison bison athabascae)
… (Chapter 5) matured oocytes to develop to the blastocyst stage in culture was evaluated in subsequent studies. In Chapter 4, the hypothesis that the morphological characteristics of wood bison cumulus-oocyte complexes (COC) affect the ability of the immature oocyte to develop in vitro following …
-
Lack of Inflammatory Response in Mouse Embryonic Cells to Listeria Infection
… has shown that key cell types within mouse blastocysts, including embryonic stem cells (ESCs) and trophoblast stem cells (TSCs), exhibit remarkable resilience against the cytotoxic effects associated with viral infections and embryotoxic cytokines, such as TNFα and IFNγ, both of which are …
-
The effects of environmentally relevant toxicants on female reproduction and embryo development
… were observed at the 2-cell, 8-cell, morula, blastocyst, and hatched blastocyst stages. Also, embryo fragmentation was assessed. Oct-4 expression (4 cell stage), E-cadherin (8-cell and blastocyst), micronucleation (4-cell, 8-cell, blastocyst), and blastocyst cell populations of the inner cell …
-
The feasibility of interspecific rescue of endothelial/hematopoietic lineage deficiency
… one species are able to survive and develop in a blastocyst from a difference species. Then we intended to make a rat-mouse chimera with an endothelium/hematopoietic system from a rat genetic background and the other tissues and organs from a mouse genetic background. The first series of …
-
Supplementing IL6, IL11, and LIF to Improve Cultured Bovine Oocyte Competency
… quality in terms of their potential to reach the blastocyst stage as well as post-transfer survival. Media formulations, oocyte quality, and inferior expression of needed transcripts are all causes of this reduced developmental potential commonly present in in vitro-produced (IVP) bovine embryos. …
-
Ultrasound-guided transvaginal follicular aspiration to provide a source of bovine oocytes for gene microinjection
… among the aspiration groups in the proportion of blastocysts produced following IVMIIVFIIVC (IX = 23.10/0, 2X = 26.1%, 2XF = 18.0%, % viable). However, the 2X group generated more oocytes and embryos throughout the experiment (2X = 83/318, 2XF = 38/211, IX = 58/2SI viable blastocyst/total oocytes; …
-
A Study of Mouse Peri-Implantation Morphogenesis
… mammalian embryo development during which the blastocyst adheres to and implants into the maternal endometrium. This process is accompanied by major remodelling events in the embryonic and extraembryonic lineages from tissue level to single cell scale. With successful implantation as …
Page 1 of 7