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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 67 for “"biotinylation"”.
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Development Of Kinase Catalyzed Biotinylation To Study Phosphoproteomics
… Our lab recently discovered kinase-catalyzed biotinylation, where ATP-biotin is utilized by kinases to label phosphopeptides or phosphoproteins with a biotin tag. To explore kinase-catalyzed biotinylation, kinetic measurements were obtained with various kinases and the data indicated that …
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Identification of TbRP2-interacting proteins using proximity-dependent biotinylation (BioID)
The protein RP2 is a tubulin cofactor C-domain containing protein with important roles in ciliogenesis. In humans, mutations in the RP2 gene are associated with 10-15% of cases of X-linked retinitis pigmentosa; a devastating disease characterised by progressive degeneration of retinal …
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Protein interaction studies on the titin A150 domain using proximity-dependent biotinylation
… behind HMERF. In this study, proximity dependent biotinylation (PDB) was used to screen for protein interactions. PDB was conducted using the biotin-ligase UltraID fused to a titin fragment. This study encompasses the creation of the titin-UltraID fusion genes, the functional validation of the …
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Spatially-resolved transcriptomic mapping in live cells using peroxidase-mediated proximity biotinylation
… spatially restricted in situ protein biotinylation with RNA-protein chemical crosslinking, while APEX-Seq utilizes peroxidase-catalyzed in situ biotinylation on RNA. We demonstrated that APEX-RIP can isolate RNAs from a variety of subcellular compartments, including the mitochondrial …
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Live cell proximity biotinylation enables proteomic analysis of mitochondria-endoplasmic reticulum contact sites
The cell is able to carry out an astounding number of biological functions in part due to its ability to compartmentalize chemical reactions to different cellular regions and organelles. In order to understand the biological pathways that occur in a specific cellular region, one needs to know the …
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Metabolic biotinylation of the adenoviral capsid: Avidin-based applications and studies of ligand-targeted gene delivery
Adenoviral vectors have great potential for use in gene therapy and genetic immunization. The targeting of Ad vectors to the relevant tissue and cell types in vivo could greatly improve their safety and performance by lowering the effective dosage required for therapeutic levels of gene expression. …
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Distribution and targeting of CLC-3
… to the plasma membrane as assessed by surface biotinylation. Plasma membrane ClC-3 was rapidly endocytosed with t1/2 of approximately 9min. Biotinylation experiment detected a portion of ClC-3 recycled back to the cell surface. Antibody binding to an external HA epitope demonstrated that the …
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Characterization of the GBF1-Arf1 axis in enterovirus RNA replication
… membranes using peroxidase-based proximity biotinylation. Viral protein biotinylation was detected as early as 3 h.p.i., and the non-cleaved fragments of the viral polyprotein were overrepresented in the Arf1-enriched domains. Furthermore, we show that after 4 h.p.i. viral proteins could be …
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Negative Regulation of the accBC Operon in Escherichia Coli
… of E. coli AccB fused to the carboxyl-terminal biotinylation domain of B. subtilis AccB complemented AccB activity and caused negative regulation of the accBC operon. Complementation of ACC activity using the malonyl-CoA synthase gene (matB) from Rhizobium trifolii allowed the deletion of the …
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Study of zein protein body formation in a heterologous system (<i>Xenopus laevis oocyte</i>)
… (proteinase K) and chemical modification (biotinylation) on isolated protein vesicles from <i>Xenopus</i> oocytes injected with <i>in vitro</i> transcribed 27K zein mRNA. In addition, the transmembrane features were analyzed by monitoring the protein's mobility in the lumen of the ER by …
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Modulation of Endothelial Cell Adhesion to Synthetic Vascular Grafts Using Biotinylated Fibronectin in a Dual Ligand Protein System
… modification to the DL system involved direct biotinylation of fibronectin (bFN) as a replacement for co-adsorption of FN with biotinylated bovine serum albumin (bBSA). This was analyzed with a Langmuir model using surface plasmon resonance (SPR) spectroscopy to verify the binding affinity of …
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Holin-dependent secretion of a large clostridial cytotoxin by C. perfringens
… FAST fluorescence technology and cell-surface biotinylation to determine if the amphipathic helix of TpeE changes its orientation in the presence of TpeL. Using FAST fluorescence, the mean pixel intensity for each frame did not change between TpeE-FAST and TpeE-FAST TpeL cultures, using a …
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An Examination Of The Cytoplasmic Dynein Stepping Mechanism At The Single Molecule Level
… protein, NeutrAvidin, in order to attach them to biotinylation sites within the dynein ring. A method was also developed to quantify the number and density of functional biotin binding sites on the nanorod surface and compare it to that of commercially available streptavidin quantum dots. These …
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Engineering bacteriophage nanocarriers for targeted delivery of protein reagents to prostate cancer cells
… protein transduction domain, and a biotinylation site on an orthogonal coat protein, which allows for complexation with streptavidin fusion proteins. Collectively, this approach permits targeted intracellular delivery of ~20 exogenous proteins per phage to human prostate cancer …
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A novel genome-wide approach to identify in vivo targets of Notch signalling
… to mice expressing the biotinylase from E. coli. Biotinylation was confirmed in a range of different tissues. However, streptavidin chromatin pull-down (bioChIP) experiments from these tissues showed no significant enrichment of known Notch1 target sequences. A possible explanation could be the …
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Development of Oligonucleotide-directed proximity-interactome MAPping (O-MAP), for characterizing RNA-protein interactions and higher order subnuclear architecture in situ
… to the target RNA enabling the promiscuous biotinylation of nearby proteins. These biotinylated proteins can be isolated by streptavidin enrichment and analyzed by downstream omics such as mass spectrometry (MS). Using the 47S pre-ribosomal RNA, long noncoding RNA XIST and 7SK small nuclear …
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Functional characterization of the RNA binding protein RALY
… using a new assay named iBioPQ (in vivo-Biotinylation-Pulldown-Quant assay). I also performed polyribosome profiling assay to check the resence of RALY in translating mRNAs. Moreover, a microarray assay was performed in order to identify potential mRNAs whose metabolism appears dependent …
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Spatially restricted proteomic mapping of excitatory and inhibitory post-synaptic termini through enzymatic labeling
… synapse to enable specific proximity dependent biotinylation of the proteomes at the glutamatergic (excitatory) and GABAergic (inhibitory) postsynaptic densities. In doing so, we provide the first selective and comprehensive survey of the inhibitory postsynaptic density, which has eluded …
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An Optogenetic Toolbox for the Interrogation of Dynamic Events During Angiogenesis
… resolution. Furthermore, the stability of biotinylation also enables the capture of weak or transient binders that may dissociate from the VE-Cadherin complex. Proteomics investigations have further supported the specificity of VE-Cadherin-LiTurboID towards endothelial and junctional …
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