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Showing 1 to 20 of 25 for “"biotin streptavidin"”.

  1. Synthetic creation of a chemotactic system via utilization of magnetically actuated microrobotic walkers

    Steimel, Joshua PaulMassachusetts Institute of Technology2012

    … the walkers. The receptor-ligand pair of biotin-streptavidin was utilized due to the extremely strong binding affinity of the pair. The presence of free biotin binding sites on the surface was required to obtain chemotactic motion as these binding sites modulated walker velocity. The …

    mit Repository record

  2. Stimulated emission from biological materials and novel optical resonators for diagnostic and imaging applications

    Rivera, Jose AUniversity of Illinois at Urbana-Champaign2017

    … and the development of a FRET-based laser using biotin-streptavidin bioconjugates comprising organic dye and gold nanoparticle donor/acceptor configurations. Furthermore, we introduce an optical resonator which can be fabricated from the directed assembly of a particle monolayer and is capable of …

    uiuc Repository record

  3. Synthesis of highly quenching fullerene derivatives for biosensor applications

    Pérez, Vanessa Virginia, 1981-Massachusetts Institute of Technology2004

    … the Stern-Volmer data. Third, the use of the biotin-streptavidin system to determine how well the fullerene derivatives would perform in a biosensor system is discussed.

    mit Repository record

  4. Cyaninfarbstoffe als Fluoreszenzsonden in biomimetischen und biologischen Systemen : Fluoreszenz-Korrelations-Spektroskopie und Fluoreszenzanisotropie-Untersuchungen

    Luschtinetz, FranziskaUniversität Potsdam

    … der Farbstoffe im jeweiligen Modellsystem. Das Biotin-Streptavidin-System wurde als Modellsystem für die Untersuchung von Protein-Ligand-Wechselwirkungen verwendet, da der Bindungsmechanismus weitgehend aufgeklärt ist. Nach Bindung der Farbstoffe an Streptavidin wurde eine erhebliche Veränderung …

    potsdam-diss Repository record

  5. Native chromatin extraction and its structure analysis

    Zhou, Zhongwu

    … purified chromatin as a source, we developed a biotin-streptavidin mediated enzyme-based immunosorbent assay for simultaneous detection of epigenetic modifications on DNA and histone proteins. In order to analyze the CARs, we also developed a magnetophoretic chromatography method to remove the …

    purdue-thes Repository record

  6. Enzymatically active microspheres for self-propelled colloidal engines

    Park, Jungeun2017

    … of the elastase-coated particles. We exploit biotin-streptavidin chemistry to couple the elastase on the surface of the PS particles. We confirm that SucAla<sub>3</sub>-pNA can be effectively decomposed by elastase and elastase-coated particles using spectrophotometric measurement. The results …

    cuny Repository record

  7. Engineering of the RTB Lectin as a Carrier Platform for Proteins and Antigens

    Reidy, Michael JamesVirginia Tech2007

    … the loss of lectin or uptake activities. The biotin/streptavidin interaction and direct genetic fusion of polypeptides also provide efficient mechanisms for the attachment of payload proteins to RTB. An immunoglobulin domain-based scaffolding mechanism bridges modified RTB and payload proteins …

    vt Repository record

  8. Gold compact disc appended Tröger’s base scaffolds as MALDI-TOF biodetection probes

    Hiatt-Gipson, GlynUniversity of East Anglia2013

    … MALDI-TOF spectrometry will be probed. The (+)-biotin / streptavidin interaction will be used as a model study for these purposes with the ambition to develop this further with humanitarian and military applications. The V-shape and 90˚ angle of Tröger’s base will be exploited to investigate its …

    east-anglia Repository record

  9. Enabling tools for biological analysis : technologies for the study of protein dynamics, detection and interaction

    Anwar, Moshiur MekhailMassachusetts Institute of Technology2007

    … interface. We use this reader to detect a biotin-streptavidin interaction using standard microarray procedures.

    mit Repository record

  10. Ionic Self-Assembled Multilayers Adsorbed on Long Period Fiber Gratings for Use as Biosensors

    Wang, ZhiyongVirginia Tech2005

    … function effectively as biosensors by using the biotin-streptavidin system. These demonstrations confirm that the ISAM-LPG scheme provides a thermally-stable, reusable, and robust platform for building efficient optical sensors.

    vt Repository record

  11. Mikroarray-basierte Detektion von mRNA aus Zellen mittels On-Chip-PCR

    Marschan, XeniaUniversität Potsdam

    … verwendet. Die affine Kopplung über Biotin- Streptavidin erwies sich als geeignet. Die On-Chip-Reaktion an kovalent gebundenen Primern wurde für amino-modifizierte Primer auf Epoxy-Oberflächen sowie für EDC-Kopplung auf silanisierten Oberflächen gezeigt. Für die letztgenannte Methode …

    potsdam-diss Repository record

  12. Selbstorganisation von Kohlenstoffnanoröhren zu Feldeffekttransistoren

    Taeger, SebastianTechnische Universität Dresden

    … Fall der Quantenpunkte gelang dies mit Hilfe der Biotin-Streptavidin Bindung unter Anwendung des Prinzips der molekularen Erkennung. Die Anbindung dieser Nanopartikel kann als Prototyp für den DNA-vermittelten Strukturaufbau aus CNT angesehen werden. Zur Deposition von CNT in Elektrodenstrukturen …

    qucosa-diss

  13. In vitro and in vivo growth factor delivery to chondrocytes and bone-marrow-derived stromal cells in cartilage and in self-assembling peptide scaffolds

    Miller, Rachel E. (Rachel Elizabeth)Massachusetts Institute of Technology2010

    … (BMSCs), respectively, by adsorption and by biotin-streptavidin tethering. This study showed that while TGF-[beta]1 can be effectively delivered by adsorption, IGF-1 can not. Additionally, while tethering these factors provided longterm sequestration, tethering did not stimulate proteoglycan …

    mit Repository record

  14. Sensitivity control of optical fiber biosensors utilizing turnaround point long period gratings with self-assembled polymer coatings

    Gifford, Erika LeaVirginia Tech2008

    … increased surface area and thickness. The biotin-streptavidin system was used as a model for implementaion and optimization of the ISAM-coated TAP LPG biosensor platform. Through evaluation of various biotin derivatives to maximize the amount functionalized onto the ISAM film, optimization …

    vt Repository record

  15. Quantenpunktbasiertes spektroskopisches Lineal mit Terbium-Komplexen als Donoren für optische FRET-Multiplexmessungen

    Morgner, FrankUniversität Potsdam

    … biomolekularen Modellsystemen Zink-Histidin und Biotin-Streptavidin beschlossen den praktischen Teil. Als Donor wurde Lumi4Tb gebunden an ein Peptid bzw. Streptavidin genutzt, Akzeptoren waren fünf verschiedene, kommerziell erhältliche Quantenpunkte mit Carboxyl- bzw. Biotinfunktionalisierung. …

    potsdam-diss Repository record

  16. In vitro Selektion von Ribozymen für eine 1,3-dipolare Cycloaddition

    Zerreßen-Harte, AndreasUniversität Heidelberg

    … funktionalisierten RNA-Konjugat und einem biotinylierten Nitriloxid katalysierten. Dabei wurde die Methode der direkten Selektion mit spaltbaren linkergekoppelten Reaktanden verwendet. Der dipolare Acrylat-Reaktant wurde über einen flexiblen Dinukleotidlinker an die RNA durch enzymatische …

    heid-diss Repository record

  17. Development of polymeric nanoparticle vaccines for immunostimulation

    Basto, Pamela A. (Pamela Antonia)Massachusetts Institute of Technology2013

    … allows selective orientation through biotin-streptavidin conjugation. Upon vaccination with CPG, selected synthetic epitopes conjugated to polymeric nanoparticles trended to reduce serum LDL-c and serum PCSK9 in murine models. Additionally, antibodies in the serum showed promise to …

    mit Repository record

  18. Fabrication of novel sensors from nanomaterials

    Small, William RichardUniversity of Hull2009

    … as the composite material). A sensor based on biotin-functionalised silver nanowires assembled into a microwire and encapsulated within agarose gel has also been fabricated. This sensor showed sensitivity to streptavidin when the response was measured parallel to the formed microwire, but gave …

    hull Repository record

  19. DEVELOPMENT OF A NEW SCREENING AND DETECTION METHOD FOR IDENTIFYING PROTEIN-SMALL MOLECULE INTERACTIONS

    Wang, Lin

    … inhibitor II-COX system. Other systems include biotin-streptavidin system and progesterone-progesterone receptor system. Several fundamental parameters (concentration, pH, etc.) that affect the detection of fluorescence anisotropy measurement are evaluated. In addition, non-specific binding of …

    siu-theses Repository record

  20. Point of care platforms for protein and nucleic acid detection

    Berger, JacobUniversity of Illinois at Urbana-Champaign2021

    … impedance cytometry, microbead immunoassays, and biotin-streptavidin binding. A multiplexed microbead immunoassay is developed and validated first for the detection of IL-6. The assay principles are further used to develop microbead based detection for PCT. The final platform demonstrates the …

    uiuc Repository record

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