Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 5 of 5 for “"biotin biosynthesis"”.
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Developing New Modalities for Biosensing using Synthetic Biology
… strong and specific interaction between biotin and (strept)avidin. Both biosensors showed high sensitivity and reliability for measuring biotin with detection limits of 50-1000 pg/ml and 20-100 ng/ml, respectively. Following these, a new biosensor was developed by coupling a mobile, …
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Metabolite biosynthetic enzymes: catalysis, substrate specificity and protein engineering
… of the enzymes that catalyze the biosynthesis of these small molecules can provide us with targets in our fight against microbes, scaffolds for their use as bioengineered tools or serve as a means to identify new biologically relevant molecules. Here I describe my efforts to (i) …
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Regulatory function and interdomain communication of group II biotin protein ligases
Biotin is a cofactor required for function of essential biotin dependent enzymes that are involved in metabolic processes including fatty acid biosynthesis, gluconeogenesis, and amino acid catabolism. In order for biotin to act as a cofactor it must first be covalently attached to the biotin …
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Studies of lipoic acid and biotin metabolism in bacteria and higher eukaryotes
… focus has been the study of lipoic acid and biotin metabolism in several bacteria. Lipoic acid and biotin are essential enzyme cofactors found in all three domains of life. Both molecules function only when covalently attached to key metabolic enzymes, 2-oxoacid dehygrogenase (OADH) and …
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Umwelt-Genomik als Quelle für die Isolierung von neuen Operons und Genclustern aus mikrobiellen Konsortien
… dieser Arbeit war die Isolierung von neuartigen Biotinbiosyntheseoperons aus mikrobiellen Konsortien mit Hilfe kombinierter Anreicherung und direktem Klonieren. Hierzu wurden Cosmid-Genbanken aus Anreicherungskulturen mit einer Fragment-Größe von ca. 30 kb angelegt. Anschließend wurden die …