Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 55 for “"biosynthetic genes"”.
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Functional Characterization of Monoterpenoid Indole Alkaloid (MIA) Biosynthetic Genes in Catharanthus roseus
… bioinformatic approaches to identify novel MIA biosynthetic genes. Virus-induced gene silencing (VIGS) is being used to screen these candidate genes for their involvement in iridoid biosynthesis pathway, especially in the identification of 7-deoxyloganic acid 7-hydroxylase (CrDL7H) shown by the …
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Statistical and Gene Regulatory Network Analysis of Monolignol Biosynthetic Genes in Populus trichocarpa.
North Carolina State University Theses Forestry & Environmental Resources.
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Molecular and functional characterization of the melanin biosynthetic genes from Vibrio cholerae 569B
… as melanin. The function of the pigment and the genes involved in its synthesis was unknown. We therefore set out to determine the function of pigmentation in V. cholerae 569B, since pigmentation could significantly enhance the survival of the bacterium during adverse conditions and therefore aid …
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DNA technology for Corynebacterium glutamicum : isolation and characterization of amino acid biosynthetic genes
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Applied Biological Sciences, 1989.
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Identification and Functional Characterization of Sesquiterpene Pheromone Biosynthetic Genes in Stink Bugs (Pentatomidae)
… bisabolene-type sesquiterpenoids; however, the biosynthetic pathways in the formation of these pheromones are unknown. Here we provide evidence that Pentatomidae produce sesquiterpene aggregation pheromones de novo and discuss the evolution of terpene biosynthesis in stink bugs. According to …
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Isolation and characterization of starch, starch biosynthetic genes and protease inhibitors from Marama beab (Tylosema Esculentum)
… and characterize marama starch biosynthesis genes and detect serine protease inhibitor activities in green and mature marama seeds. The total starch content of marama bean tubers was determined by amyloglucosidase/α-amylase enzymatic digestion and amylose content by Concanavalin A …
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Investigating the role of glutathione and glutathione biosynthetic genes in the adaption of Anopheles gambiae to insecticides
… this work examines the contribution of GSH, its biosynthetic genes (<i>GCLM</i>, <i>GCLC</i>) and their possible transcriptional regulator <i>Nrf</i>2 in insecticide resistance in <i>An</i>. <i>gambiae</i> sampled from agricultural setting (areas of intensive agriculture) and residential setting …
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Characterization of the expression profile of polyamine biosynthetic genes (Spermidine synthase) and polyamine metabolic regulation in arabidopsis
… aimed at elaborating the regulation of polyamine biosynthetic gene expression and polyamine metabolism. The organ/tissue specific expression patterns of two genes encoding the polyamine biosynthetic enzyme spermidine synthase ( AtSPDS1 and AtSPDS2) were studied in Arabidopsis at different …
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Characterization of an operon containing a ribosomal protein gene and lipid biosynthetic genes in Escherichia coli K-12
… that the rpmF-plsX-fabH-fabD-fabG-acpP genes comprise an operon suggesting a coordinate control of the synthesis of a ribosomal protein (L32), PlsX protein, and fatty acid biosynthetic enzymes. However, several features were identified that are likely to be important for differential …
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Characterization of the A/B regulon in tobacco (Nicotiana tabacum)
… biosynthesis in N. tabacum by identifying genes that are coordinately regulated during conditions that induce alkaloid biosynthesis and by comparing their expression in regulatory mutant backgrounds that differ at two quantitative alkaloid loci, A and B. In order to identify novel genes …
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BIOCHEMICAL AND MOLECULAR CHARACTERIZATION OF RAFFINOSE FAMILY OLIGOSACCHARIDE (RFO) BIOSYNTHESIS IN LENTIL (Lens culinaris Medik.)
… this study was to isolate and characterize RFO biosynthetic genes galactinol synthase (GS or GolS; EC 2.4.1.123), raffinose synthase (RS; EC 2.4.1.82) and stachyose synthase (STS; EC 2.4.1.67). The expression of RFO biosynthetic genes, corresponding enzyme activity and RFO accumulation were also …
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Characterization of the Diverse Substrate Specificities and Biological Roles of Polyamine Biosynthetic Enzymes in Microorganisms
… and functional diversity in bacterial polyamine biosynthetic decarboxylases than previously suspected. The beta /alpha -barrel fold decarboxylases also include a group of bacterial putative carboxynorspermidine decarboxylases (CANSDCs), which were hypothesized to be involved in norspermidine …
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Elucidating the Biosynthesis of Nucleocidin, a Fluorinated Natural Product Produced by Streptomyces Calvus
… glycosylation of fluoride. The nucleocidin biosynthetic gene cluster has recently been identified in Streptomyces calvus. This provides an opportunity to elucidate the functions of the encoded enzymes. To determine the minimal set of genes required to synthesize nucleocidin, a strategy was …
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Coordinated of Post-Translational Modifications of Yeast Transcriptional Activator Gcn4
… activator that regulates amino acid biosynthetic genes in yeast. Gcn4 is expressed under amino acid starvation conditions, and its degradation depends on phosphorylation, by either Pho85 or Cdk8, which triggers its ubiquitination and subsequent proteolysis by 26S proteasome. Previous …
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Pathway engineering for the discovery and optimized production of phosphonic acids
… the abundant wealth of available genomic data, biosynthetic gene clusters can be readily identified for exploitation. However, two bottlenecks impede the transition from identified gene cluster to useful drug. The first hurdle is identifying the molecule linked to a set of biosynthetic genes. …
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Phenotypic and genotypic characterisation of invasive Streptococcus pneumoniae expressing atypical capsular types
… virulence factors, and antibiotic-resistance genes present, including whole genome sequencing-inferred minimum inhibitory concentration where applicable. Analysis of the capsular loci for single nucleotide polymorphism mutations were also conducted. Nuclear magnetic resonance was used to …
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Identifying biosynthetic pathways for mycobacterial cell wall components using transposon mutagenesis
… wall. Several anti-TB drugs target cell wall biosynthetic pathways. A good understanding of its biosynthesis will provide helpful clues for the development of novel drug targets. A strategy based on random transposon (Tn) mutagenesis with two different screening criteria, altered colony …
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Bio-enrichment of vitamin B12 in plant-based food via fermentation
… with added precursors. Despite having complete biosynthetic genes, some strains failed to produce active B12 in vitro and in food. Co-fermentation with Propionibacterium increased and accelerated B12 production compared to monocultures, while also moderating acidification and improving vitamin …
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Isolation and characterization of a synthetic gene and a genomic clone encoding acyl carrier protein from Escherichia coli
… fabF and fabD which encode two fatty acid biosynthetic enzymes, 3-ketoacyl-ACP synthase II and malonyl CoA-ACP transacylase, respectively. An open reading frame between acpP and fabD encodes a 26.5-kDa protein that has significant sequence identity ($>$40%) with a plant 3-ketoacyl-ACP …
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Investigating the biosynthesis of the streptomycete antibiotic pacidamycin
… unexploited mode of action against a cell wall biosynthetic enzyme target. This thesis reports the investigation into the biosynthesis of pacidamycin, more specifically, into the function of the hypothetical protein genes present in the pacidamycin gene cluster and the biosynthesis of the …
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