Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 34 for “"biosynthetic gene cluster"”.
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Molecular Analysis of the Essential Fatty Acid Biosynthetic Gene Cluster of Escherichia Coli
The genes encoding ACP and several key fatty acid biosynthetic enzymes are clustered at min 24 of the E. coli chromosome in the order of plsX, fabH, fabD, fabG, acpP, and fabF. I demonstrated that this cluster of genes was transcribed as a non-conventional operon. Multiple promoters were present …
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Structure, Regulation and Comparative Biochemistry of the Pyrimidine Biosynthetic Gene Cluster in Bacillus Subtilis (Atcase)
… encoding the entire Bacillus subtilis pyrimidine biosynthetic (pyr) gene cluster was cloned in Escherichia coli. The cloned fragment also complemented E. coli pyrB, C, D, E, and F mutants. A restriction map of the cluster was generated, and many subclones were analyzed for their ability to …
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L-arginine overproduction in Corynebacterium glutamicum ATCC 13032
… of producing L-arginine commercially. A novel gene replacement system was initially used for the directed mutation of the Larginine biosynthetic gene cluster in C. glutamicum ATCC 13032. This was met with limited success, however, and the pK19mobsacB vector was thus adopted for further …
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Biosynthesis of Fluorinated Natural Product Nucleocidin
… glycosylation of the fluoride ion. Although the gene cluster encoding for nucleocidin has been predicted, the fluorinase gene remains unknown. The biosynthetic gene cluster of nucleocidin has been successfully cloned into a phage P1 artificial chromosome library of the S. calvus genomic DNA, …
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Oxygen-dependent enzymes involved in phosphonate biosynthesis
… we now have the ability to quickly identify new biosynthetic gene clusters with the capacity to produce new natural products and quickly identify the genes required for the production of a natural product. Characterization of these biosynthetic gene clusters allow us to identify the enzymes …
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Characterization of Cyclopropyl Synthases Involved in the Maturation of Ribosomally Synthesized and Posttranslationally Modified Peptides
… implications. RiPPs are synthesized from a genetically encoded precursor peptide that undergoes significant modifications by maturing enzymes, or maturases. Recently, radical-S-adenosylmethionine (rSAM) enzymes have emerged as an important family of RiPP maturases. rSAM enzymes have been …
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Identification and Characterization of Antibiotic Biosynthetic Gene Clusters in Pantoea
… search has been predominantly limited to a few genera. One underexplored bacterial genus is Pantoea, which produces a variety of compounds with antimicrobial activity. Chapter one describes a general overview of secondary metabolites, antibiotic resistance, strategies to overcome resistance, and …
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Elucidation of gene clusters underlying withanolide biosynthesis in ashwagandha
… and adaptogenic properties. However, the biosynthetic pathway to withanolides is largely unknown, preventing scale-up and hindering pharmaceutical applications. In this thesis, we report a chromosome-scale assembly of the W. somnifera genome, which we use for biosynthetic gene cluster …
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Reengineering and Discovery of Nonribosomal Peptide Synthetases
… sequencing and characterization of a novel NRPS biosynthetic gene cluster using this new proteomics based platform in the Kelleher lab is described in Chapter 4. In summary, this thesis is founded on and extends upon the pioneering work of in vitro characterization of NRPSs performed in the …
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Insights into the Synthesis, Mechanism of Action, and Biosynthesis of a Novel Lipodepsipeptide Antibiotic, WAP-8294A2
… of action and identify and characterize the biosynthetic machinery.</p><p>We developed a solid-phase strategy for the development of WAP-8294A2 and analogs with on-resin cyclization based on molecular modeling studies. We chose a macrocyclization site between Glu8 and Asn9 to take advantage …
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Genome Mining for Actinomycete Biosynthetic Gene Clusters
… (8-12 Mbp) genomes of Streptomyces and allied genera of Gram-positive filamentous bacteria house a rich and previously underestimated repertoire of gene clusters for biosynthesis of specialised metabolites, including antibiotics, immunosuppressants and anticancer compounds. Many of these …
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Genomics as a tool for natural product structure elucidation
… Bacillus anthracis harbor mutations to walR, the gene encoding a response regulator for the only known widely-distributed and essential two-component signal transduction system in Firmicutes. Our results demonstrate that understudied microbes remain fruitful reservoirs for the rapid discovery of …
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Functional Analysis of Secondary Metabolite Biosynthesis-Related Genes in Alternaria brassicicola
… from knockout mutations of each PKS and NPS gene was investigated with an emphasis on discovery of fungal virulence factors. A highly efficient gene disruption method using a short linear double stranded DNA construct with minimal elements was developed, optimized, and used to functionally …
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Biochemical and Functional Characterization of Induced Terpene Formation in Arabidopsis Roots
… and oospore germination in vitro, and that DMNT biosynthetic mutant plants were more susceptible to P. irregulare infection. We provide evidence based on genome synteny and phylogenetic analysis that the arabidiol biosynthetic gene cluster containing the arabidiol synthase (ABDS) and CYP705A1 …
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Ecology and Synthetic Biology as Tools for Natural Products Discovery From Marine Proteobacteria
… in genomic sequencing, genomic analysis, and genetic engineering, it has become clear that only a small portion of the bioactive secondary metabolites that marine bacteria are capable of producing have been characterized. This thesis is an effort to aid in uncovering new chemistries and new …
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Biomolecular NMR studies of the structure and biosynthesis of RiPP natural products
… compounds are broadly defined as small molecules generated by organisms from multiple kingdoms including plants, fungi, and prokaryotes. The outsized impact natural products have had on modern medicine has inspired ongoing efforts to find and characterize new natural products. The vast increase in …
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Elucidating the Biosynthesis of Nucleocidin, a Fluorinated Natural Product Produced by Streptomyces Calvus
… glycosylation of fluoride. The nucleocidin biosynthetic gene cluster has recently been identified in Streptomyces calvus. This provides an opportunity to elucidate the functions of the encoded enzymes. To determine the minimal set of genes required to synthesize nucleocidin, a strategy was …
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Investigations of Carbapenem-resistant Klebsiella species and associated clinical considerations
… blaTEM-1, blaOXA and blaCTX-M-15, and the 12- gene operon of the kleboxymycin biosynthetic gene cluster. This gene cluster encodes for tilimycin and tilivalline, enterotoxins previously thought only to be carried by K. oxytoca strains. Incorporation of antimicrobial resistance and virulence …
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The biosynthesis and discovery of lanthipeptides
… To add onto the structure knowledge and biosynthetic toolkits for lanthipeptides, I investigated the biosynthesis and discovery of lanthipeptides during my Ph.D training. Duramycin/cinnamycin-type of lanthipeptides interact tightly with phosphatidylethanolamine and several of their …
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