Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 27 for “"bioproduction"”.
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Organic acids bioproduction from corn fiber using Actinobacillus succinogenes
… of sugars. The aims of this study included SA bioproduction, investigation of the effect of N₂ and CO₂ bubbling on LA production, and LA bioproduction from corn fiber (CF) hydrolysates using A. succinogenes. CF is a byproduct of the dry grind corn ethanol process and has minimal lignin and high …
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Bioproduction Optimization and Characterization of Hyaluronic Acid in Escherichia coli and Nicotiana benthamiana
Hyaluronic Acid (HA) is a polymer widely used in biomedical and pharmaceutical applications, with its biological and physical properties strongly dependent on molecular weight. Conventional production methods, including animal tissue extraction and Streptococcus fermentation, yield high-molecular …
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A Study of Polymer-Small Molecule Interactions for Solid-Liquid Two Phase Partitioning Bioreactors With Emphasis on the Bioproduction of 2-Phenylethanol
… TPPB systems were able to greatly enhance bioproductions associated with end product inhibition in terms of final product concentration and productivity. The ease of operation is also attractive compared to two liquid phase systems.
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Polymeric Chemisorbents for Organic Acid Recovery from Aqueous Solution
… acid (SA) from fermentation media during bioproduction makes it difficult for bioproduced SA to compete with petrochemically-derived SA. Two new polymeric sorbents and multiple recovery methods that have the potential to reduce bioproduction costs are examined. The first sorbent is a …
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Genetic and Computational Tools for Engineering Complex Prokaryotic Systems
Prokaryotic systems are promising platforms for bioproduction, but the complexity of their gene regulatory and metabolic networks presents significant challenges for engineering efficient biosynthetic pathways. While CRISPR-Cas technologies have revolutionized genome editing in eukaryotes, the …
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Precise and expansive genomic positioning for CRISPR edits
… genetic research, diagnostics, therapeutics, and bioproduction. An ideal DNA-editing platform would achieve perfect accuracy on any desired cellular and genomic target. CRISPR systems, however, have limited target fidelity and range, in part due to their evolutionary pressures to defend microbes …
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The Synthesis of Succinic Acid and its Extraction From Fermentation Broth Using a Two-Phase Partitioning Bioreactor
… fine and commodity chemicals. No commercial SA bioproduction process exists due to process limitations including end product inhibition and high product separation costs, which account for 70% of total production costs. Two-Phase Partitioning Bioreactors (TPPBs) can increase volumetric …
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Nanoelectroporation of single cell plants
… offer a promising, chassis for the scalable bioproduction of biologics. The prospects for distributed and low-cost production offer the potential to support rapid responses to emerging diseases and tackle global health inequity. This thesis aims to support the use of M. polymorpha as a model …
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Plant-derived Murine IL-12 and Ricin B-Murine IL-12 Fusions
… application is limited by lack of an effective bioproduction system and by toxicity associated with systemic administration of IL-12. The goals of this research were to determine whether plants can serve as an effective production system for bioactive IL-12, a complex 70kDa glycoprotein …
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Celulases para produção de etanol de segunda geração
… impact. The use of lignocellulosic biomass to bioproduction has become vital to the economy due to their renewable nature, abundance and low cost. The lignocellulosic residues (bagasse and straw) from the sugarcane are abundant sources of carbohydrates and their bioconversion has received great …
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ENGINEERING WATER-SOLUBLE VARIANTS OF THE SINGLE-SUBUNIT OLIGOSACCHARYLTRANSFERASE
… water-soluble N-OST enzymes with applications in bioproduction of the glycotherapeutics and glycovaccines.
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The Expression and Characterization of Human Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) in Tobacco
… expensive and low yielding. However, successful bioproduction of many complex human proteins has been shown in transgenic plants. Our research objective is to develop tobacco as a model system for expressing human CFTR. Constructs of full-length CFTR fused to the 35S double enhanced promoter …
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Synthetic biology approaches for engineering bacteria as living therapeutics
… pathogen Clostridium difficile and to serve as a bioproduction chassis of the C. difficile-targeting peptide. Together, this work outlines the development of a platform for creating the next-generation of living therapeutics and a unique method for engineering collections of bacteria to identify …
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Development and application of a microfluidic platform for single-cell cultivation of CHO suspension cell lines
… hamster ovary (CHO) cells are utilized in these bioproduction processes, as they can easily be genetically modified and thereby are able to express biopharmaceuticals that are human-compatible, are well-established in industrial-scale cultivation, and achieve high product titers. Yet especially …
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Messa a punto di processi biotecnologici per la produzione di biocombustibili da matrici organiche di rifiuto
The bioproduction of materials and energy from renewable sources (industrial biotechnology) is getting more and more interest in order to improve environmental sustainability of chemical industrial processes and to decrease their dependence from oil. Anaerobic digestion of organic waste matrices …
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Towards value from waste: Bioreactor selection for the reduction of nutrient load and production of Poly-y-glutamic acid
… merging of integrated wastewater processing with bioproduction of valuable products from a waste stream, whilst still achieving clean water as an equally important and valuable product. These value-add products need to have sufficient value, and fulfil a market need to ensure that the WWBR is …
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Metabolic engineering of xylose-fermenting saccharomyces cerevisiae for bioisoprene production
… sugar, xylose, is a nascent approach for bioproduction of commodity chemicals. Since xylose, the second most abundant sugar after glucose, can be assimilated by microorganism as a cheap carbon source to make ethanol and other chemicals, bioisoprene production out of xylose is not …
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The development of Sinorhizobium meliloti and Deinococcus radiodurans as chassis for synthetic biology applications
Microorganisms can be harnessed for bioproduction and biotechnology to further global efforts in agriculture, health, manufacturing and sustainability. Traditional microbial chassis used for these purposes are the most well-characterized bacteria and yeast, Escherichia coli and Saccharomyces …
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STUDIES ON NATURAL AND EDIBLE BIOPOLYMERS. ISOLATION, CHARACTERIZATION AND CHEMICAL MODIFICATIONS OF POLY(Γ-GLUTAMIC ACID) (Γ-PGA) FROM BACILLUS SUBTILIS
… Western world. This Ph.D. thesis deals with the bioproduction, characterization and derivatization of γ-PGA from Bacillus subtilis in order to obtain novel biomaterials useful for biopackaging, biomolecule immobilization, and as drug carriers. In particular, the problems we have addressed were: …
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Fermentation of organic waste for bioplastic production : A potential sustainable alternative to fossil-fuel plastic
… lies the relevance of developing the bioplastic bioproduction and technology. The present research focused on the development of a specific production of polyhydroxyalkanoates (PHAs) from organic waste, in collaboration with the start-up Dionymer (Bordeaux, France). First, the purpose of the …
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