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Showing 1 to 20 of 70 for “"biophysical characterization"”.
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Functional dna: Biochemical/biophysical characterization & sensing applications
… of the 8-17 DNAzyme and involve biochemical and biophysical characterization of the 8-17 DNAzyme as well as characterization of a novel red Pb2+ species formed upon cleavage of a modified 8-17 DNAzyme. Determination of the enantiomeric ratio is important as many monetarily and functionally …
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Biophysical characterization of the Escherichia coli RNA degradosome
… by crosslinking onto the lipid membrane. Using biophysical methods, the association and dissociation constants and the thermodynamic parameters of the interactions of the degradosome with the lipid membrane were measured. To investigate in detail on the helicase activity of the RNA degradosome, …
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Biophysical characterization of biomolecular condensates through microfluidic technologies
… and neurodegenerative diseases. However, key biophysical questions still exist as to how condensates are able to remain stable in cells, how the transition process from individual molecules to micron-scale condensates occurs, and the exact nature of the interactions of multiple heterogeneous …
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Biophysical Characterization Of A Proline Mutant Of Neural Cadherin
Cadherins are calcium dependent glycoproteins whose homophilic interactions mediate cell-cell adhesion in solid tissues. They are comprised of an extracellular region, a transmembrane region and a cytoplasmic region. The extracellular region plays a critical role in cadherin-mediated cell adhesion, …
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The Novel Purification and Biophysical Characterization of CXC Chemokines
… quantities for thermodynamic and kinetic biophysical characterization experiments. The results show, that the CXCL chemokines folds and self-associates into homodimers that can activate a membrane bound CXC Receptor, at concentrations above 20 M. Interestingly, the tested CXC ligands …
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Multi-Constriction Microfluidic Sensors for Single-Cell Biophysical Characterization
… to classify different cells based on their biophysical traits which can be utilized as a valuable tool for testing responses to physical and chemical stimuli.
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Biophysical characterization of KO513, a protein expressed in human invasive glioblastoma
… objective of the current study was to perform biophysical characterization of K0513. Characterization of K0513 will allow structure-function annotation which may serve as a basis for establishment of K0513 as a potential biomarker for GBM. Using bioinformatics analysis, a potentially functional …
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DISCOVERY AND BIOPHYSICAL CHARACTERIZATION OF ALLOSTERIC INHIBITORS OF FACTOR XIa (FXIa)
Thrombosis is one of the leading causes of mortality and morbidity that is associated with myocardial infarction, stroke and pulmonary embolism. Anti-thrombotic agents which intend to reduce the occurrence and severity of thrombosis usually target the enzymes of the coagulation cascade. FXIa, a 160 …
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Biophysical Characterization of Structure and Dynamics of Nuclear Pore Complex Components
<p>The Nuclear Pore Complex (NPC) mediates nucleo-cytoplasmic transport in all eukaryotes and is among the largest cellular assemblies of proteins, called nucleoporins (nups). The details of NPC architecture, dynamics, and mechanism are still unknown. NPCs can be dissected biochemically into …
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Biophysical Characterization of SNARE Complex Disassembly Catalyzed by NSF and alphaSNAP
Fuer die Fusion intrazellulaerer biologischer Membranen sind Vertreter der konservierten Familie der SNARE-Proteine von essentieller Bedeutung. Man vermutet, dass gerichtete Komplexbildung zwischen SNAREs auf gegenueberliegenden Membranen dazu fuehrt, dass die zu fusionierenden Mebranen in …
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Biophysical characterization of high affinity engineered single chain Fv antibody fragments
… through directed evolution were probed using biophysical techniques to illuminate affinity modulation in proteins. First, anti-c-erbB-2 antibodies and their binding partner, the extracellular domain of the glycoprotein tumor antigen c-erbB-2, were examined. Thermodynamic studies were carried …
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Biophysical characterization of hydrogel-core, lipid-shell nanoparticles (nanolipogels) for HIV chemoprophylaxis
Nanoparticles are emerging as versatile vehicles for drug delivery, providing targeting, protection, and controlled-release capabilities to encapsulated cargo. Polymeric nanoparticles made from poly(lactide-co-glycolide) (PLGA) are biodegradable, exhibit tunable drug release, and have encapsulated …
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Probing Convergence in Orthogonal Conjugated Catalysis by T4 Lysozyme Utilizing Biophysical Characterization
… 2-EP and has been investigated using various biophysical methods to probe the convergence between glycosidic hydrolase classes in their ability to produce this conjugated polymer. Though the polymerization mechanism remains elusive, this initial work suggests these systems may be adaptable for …
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Biophysical Characterization of the vOTU Proteases from the CCHF and Dugbe Nairoviruses
<p>My research focuses on understanding the substrate specificity of the viral homolog of the ovarian tumor domain protease superfamily (vOTU) from nairoviruses, and the structural reasons for their specificities. The vOTU from the Crimean-Congo Hemorrhagic Fever Virus (CCHFV) has been implicated …
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Spontaneous activity in the mouse visual cortical slice: biophysical characterization and pathophysiology
… supporting this hyperactivity, but also of the biophysical mechanisms driving the activity itself, as each remains relatively unexplored. In Chapter 1, I review relevant FXS pathophysiology and our understanding of the various forms of spontaneous activity generation in neocortical brain slices. …
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Biophysical Characterization of Tryptophan Locales, Mg²+ Binding and Protein Folding in Gα Subunits
<p>The objective of this study is to understand the structure of guanine nucleotide - binding (G) proteins using a variety of spectroscopic tools. G proteins are membrane-bound proteins consisting of α, β, and γ subunits required for the transduction of extracellular signals to various …
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