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Showing 1 to 3 of 3 for “"bioorthogonal labeling"”.

  1. Bioorthogonal Tethering of Drug Fragments to Engineered G Protein-Coupled Receptors

    … employing genetic code expansion (GCE) and bioorthogonal chemical reactions for tethering drug fragments adjacent to allosteric sites in GPCRs to increase their potency and enable fragment-based drug screening in cellular systems. C-C chemokine receptor 5 (CCR5) was selected as a model …

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  2. Development of Novel Chemical Biology Tools for Probing Structure-Function Relationships in G Protein-Coupled Receptors

    … thesis project was to develop a strategy for the bioorthogonal labeling of GPCRs with useful chemical probes or chemically reactive handles at specific defined sites. Several strategies were explored to label unnatural amino acid residues genetically encoded into GPCRs expressed in mammalian …

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  3. Analysis of Small Molecule Inhibitors Aimed at Bacterial Virulence

    … several alkynyl SAH analogs and employed bioorthogonal labeling techniques for proteomic analysis of their protein-binding partners. Through structure-activity relationship (SAR) analysis of the alkynyl analogs, I discovered important features for the inhibitory activity of the SAHs and …

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