Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 4 of 4 for “"bioorthogonal chemical reporters"”.
-
Bioorthogonal Chemical Reporters Reveal Fatty-Acylation of Histone H3 Variants and Cholesterol Modification of Proteins and Trafficking in Cells
… has remained difficult for decades. Here, using bioorthogonal chemistry, we have developed a robust system to identify the proteins modified by lipids and advanced the ability to image cholesterol in cells and tissues. The unbiased proteomic analysis of fatty-acylated proteins using chemical …
-
The Development of Novel Tools for the Metabolic Labelling of Glycans in Cancer
… by incorporating unnatural sugars bearing bioorthogonal chemical reporters. Methylcyclopropenes are one such reporter with good incorporation and kinetics of the subsequentinverse-electron-demand Diels-Alder reaction (IED-DA) with tetrazines. The methylcyclopropene tagged monosaccharide …
-
Targeted Imaging Agents for Detecting Tumour Cell Death following Therapy
… module (within 58±5.8 min (n = 12), >98% radiochemical purity and 12±3% decay corrected yield) has been developed. This was used to label the single cysteine present on C2Am within 30 min in PBS (Am=212000±30000 MBq/µmol (n = 3)). Using xenograft models of breast and colorectal cancer, and a …
-
Develoment and Application of Chemical Strategies to Study Protein Fatty-Acylation in Eukaryotes
… of PATs and palmitoylomes, I developed bioorthogonal chemical strategies for improved analysis of dynamic palmitoylation in mammalian cells. I showed that alkyne-functionalized fatty acids, in conjunction with azido-fluorophores, provide the most sensitive detection of acylated proteins …