Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 41 for “"biomolecular condensates"”.
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Biomolecular Condensates in Transcriptional Regulation
… mysterious for decades. However, recent study of biomolecular condensates has reframed our understanding of these cooperative interactions. Condensates are membrane-less compartments that concentrate components involved in the same biochemical processes. This thesis examines the formation and …
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Understanding and designing complex architectures of biomolecular condensates
Biomolecular condensates emerge when a homogeneous liquid phase separates into the condensate and a dilute phase. Condensates in cells function as membrane-less organelles and perform a variety of (temporary) functions. They may also be involved with pathological processes, like the progression of …
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Biophysical characterization of biomolecular condensates through microfluidic technologies
Biomolecular condensates can form through phase separation when mixtures of pro teins and nucleic acids coalesce to form membrane-less sub compartments within the cell. These condensates have been indicated to be responsible for a range of key physiological and pathological indications in living …
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Linking Biomolecular Condensates to Disease and Therapeutic Development
… the notion that membraneless organelles, called biomolecular condensates, compartmentalize biomolecules – proteins and nucleic acids – involved in shared cellular processes through a biophysical process called phase separation. Biomolecular condensates have distinct physicochemical properties …
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Insights from biomolecular condensates into disease and drug development
… and nucleic acids into diverse phase-separated biomolecular condensates. The study of condensates has yielded myriad fundamental insights into cell biology, ranging from a better understanding of cellular organization, to the exploration of novel mesoscale functions resulting from the emergent …
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Direct Computations of Spatially Resolved Viscoelastic Moduli of Biomolecular Condensates
<p>Biomolecular condensates are viscoelastic materials formed by liquid-liquid phase separations (LLPS) of biopolymers. In this study, we develop a modified graph Laplacian-based collective model to characterize viscoelastic heterogeneity within condensates based on results of lattice-based …
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Biomolecular Condensates Through a Computational Microscope Design, Mechanisms and Implications
Biomolecular condensates, ubiquitous in biology, are crucial in organizing cellular functions and regulating various biochemical processes. In this thesis, I explore the emergent behaviours of these biomolecular systems via computational simulations and biophysical insights. I quantify and describe …
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Engineering Synthetic Biomolecular Condensates with Rationally-Designed Modular Repeat Proteins
Biomolecular condensates (BCs) are membraneless organelles recently recognised as crucial regulators of numerous cellular processes, essential for cellular fitness and dynamic adaptation to environmental changes. They facilitate spatiotemporal control in critical cellular functions, including …
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Characterization and Modulation of the Emergent Material Properties of Biomolecular Condensates
… reconstituted in-vitro model systems of protein condensates and advance current methodology in order to address these fundamental questions. The first model system examined is the neuronal granule protein Fragile X Mental Retardation Protein (FMRP), an RNA-binding protein, that aids in the …
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Investigating the dynamic nature of biomolecular condensates that support rotavirus replication
… have been recognised as ribonucleoprotein condensates that serve as replication sites for RVA, many aspects of their formation, functions, and composition remain poorly understood and require further investigation. Utilising a machine learning-based LLPS prediction tool in combination with …
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Advancing Small Molecule Drug Discovery by Targeting Biomolecular Condensates and Evaluating Novel Chemical Scaffolds
… the feasibility of targeting “undruggable” condensates and exploiting unique chemical moieties for potential therapeutic outcomes, challenging the boundaries of modern drug discovery.
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Discovering non-equilibrium mechanisms that regulatestructure and function of biomolecular condensates usingphase-field modeling
Biomolecular condensates are phase-separated assemblies in living cells that form through cooperative interactions between their constituents such as proteins and RNAs. They are emerging as important organizers of biochemistry in cells and are dysregulated in disease. Understanding the physical …
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Recruitment of Enzyme Cascade to Phase-Separated Biomolecular Condensates Accelerates Reactions via Concentration-Dependent and Concentration-Independent Mechanisms
Biomolecular condensates are ubiquitous throughout biology, but their functions remain largely poorly understood. Biomolecular condensates concentrate biomolecules relative to the surrounding medium. For biomolecular condensates that concentrate enzymes and their substrates, classic enzyme kinetics …
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mRNA localization in Caenorhabditis elegans embryogenesis
… formation, but are actually phase-separated, biomolecular condensates. Due to their liquid-like nature, biomolecular condensates can aggregate or disperse component transcripts and proteins with exquisite environmental and temporal sensitivity. As a result, biomolecular condensates can …
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Quantitative Studies of Composition and Formation of Yeast P Bodies
… being enclosed by membranes--these are termed biomolecular condensates. Quantitative studies are lacking to mechanistically understand condensates within the complicated cellular environment. Toward this aim, I developed live cell imaging methods to quantitatively measure protein partitioning …
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Investigating Ageing of Neurodegenerative Disease Relevant Protein Condensates
… that can undergo the LLPS process and form biomolecular condensates. It has been demonstrated repeatedly that these condensates can play a wide range of cellular roles, from transcription to metabolism. In addition, an increasing amount of research is devoted to investigating aberrant …
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The Effects of DEAD-box Helicase Activity on Biomolecular Condensate Structure and Dynamics
Biomolecular condensates are membraneless organelles which help spatiotemporally organize the biochemistry of the cytoplasm and nucleoplasm. These bodies are composed of specific proteins and RNA which self-associate to drive condensate formation. The strengths, timescales, and valence of the …
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Characterizing unique features of prion-like domains recruited to stress granules
… each exposed β-strand. Solid-state assemblies in biomolecular condensates such as the Balbiani Body in oocytes share a similar β-sheet rich structure that contributes to low dynamicity of the interactions in this separated phase. Prion-like domains (PrLDs) are domains that compositionally resemble …
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Targeting and Manipulating Endogenous Transcriptional Condensates
Biomolecular condensates are membraneless compartments composed of selectively concentrated biomolecules with liquid-like properties. In this thesis, we discuss our discoveries showing that biomolecular condensates are associated with transcription. However, tools to study transcriptional …
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Investigating processing body condensation, material properties and function during early Drosophila development
… compartments. The latter, commonly known as biomolecular condensates, often contain ribonucleoprotein assemblies that form via liquid-liquid phase separation. Conserved across eukaryotes, Processing bodies (P bodies) are cytoplasmic biomolecular condensates which act as hubs for RNA …
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