Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"bioluminescent imaging"”.
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Development of in vivo tumour models for non-invasive proof-of-principle investigation of novel therapeutic agents. Engineering and characterisation of bioluminescent cell reporter systems for in vivo analysis of anti-cancer therapy pharmacodynamics.
… of cancer cells to express fluorescent or bioluminescent proteins, either endogenously or under the control of specific gene promoters, and their detection by noninvasive optical imaging has the potential to improve preclinical drug development. In this study, a panel of colorectal cancer …
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Combination Chemotherapy and In vivo Modeling of BRCA-deficient, High-grade Serous Ovarian Cancer
… impact of BRCA status on therapeutic efficacy, a bioluminescent, BRCA-deficient xenograft model was developed. The model demonstrated characteristics resembling the disease clinically, including high disseminative tumor pattern, ascites production, and platinum sensitivity. Bioluminescent imaging …
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A Developed and Characterized Orthotopic Rat Glioblastoma Multiforme Model
… longitudinal tumor monitoring in vivo through bioluminescent imaging. I have characterized their response to H-FIRE relative to DI TNC1 rat astrocytes. I have demonstrated the presence of the molecular targets of QUAD in F98 cells. The in vitro characterization of this model has enabled …
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Engineered Tracking and Delivery of Mesenchymal Stem Cells (MSCs)
… in only the irradiated leg as detected by bioluminescent imaging (BLI). The biolumescence increased over time, indicating that the engrafted MSCs proliferated <i>in vivo</i>. In contrast, cells delivered intravenously were trapped in the lungs with no engraftment. This homing is specific to …
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The Immunogenicity of Cholangiocyte Cellular Therapies
… expressing luciferase to allow for real-time bioluminescent imaging in vivo. I characterised the expression of HLA molecules on COs compared to primary cholangiocytes and after exposure to a series of pro-inflammatory environments and demonstrated that expression of HLA molecules is reduced in …
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Employing T Cells For Antigen Presentation: Role of Ny-Eso-1+ T-Apc Vaccine In Multiple Myeloma
… + T-APC group showed similar tumor delay using bioluminescent imaging, but improved tumor control on necropsy compared to the NY-ESO-1<sup>+</sup> CAR alone group. The NY-ESO-1<sup>+</sup> CAR + T-APC group also showed increased persistence of CAR<sup>+</sup> T cells with memory phenotype …
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Novel strategies to prevent and treat experimental pneumococcal disease
… on disease progression. Real-time photonic imaging of bioluminescent bacteria offers significant advantages over conventional methods for monitoring and combating bacterial disease in animals. Not only does this approach reduce the time and costs associated with such experiments, but also it …
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Preclinical Models to Identify Molecular Drivers of Metastasis in Paediatric Musculoskeletal Cancer
… tissues identified as being positive by in-vivo bioluminescent imaging. The FACS-based method allows for selective isolation of tumor cells from primary and secondary sites as well as from circulating tumor cells in the blood and enables high-throughput downstream genomic analysis. Using …
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Anti-inflammatory drugs for modulation of host response to biomaterials and application in diabetes therapy
… Firstly, we developed a new non-invasive in vivo imaging technique to study the activity of early immune cells in the host response to implanted biomaterials. A fluorescent imaging probe (Prosense680*, Perkin Elmer) activatable by cathepsins, a class of inflammatory proteases secreted from immune …
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CHARACTERIZING THE ROLE OF PUTATIVE VIRULENCE GENES ASSOCIATED WITH INFECTION, COLONIZATION AND PERSISTENCE OF SALMONELLA ENTERITIDIS IN CHICKEN USING A BIOLUMINESCENT REPORTER
… after oral gavage in day-old chicken, using the bioluminescent imaging (BLI) technique. SEn was genetically engineered to continuously produce light (490nm) using the light-emitting module called lux operon. This technique has been widely used in various bacteria enabling live imaging in mouse …
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The Role of Acidic Fibroblast Growth Factor and Fibroblast Growth Factor Receptor 4 In High Grade Serous Carcinoma
… protein. Tumor progression was then followed via bioluminescent imaging. The FGFR4 gene from 52 clinical samples was sequenced to screen for mutations.</p> <p>Results: FGFR4 DNA and mRNA copy numbers were significantly correlated and FGFR4 DNA copy number was significantly correlated with that of …