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Showing 1 to 20 of 55 for “"bioluminescent"”.
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Syntheses of luciferins and their bioluminescent evaluation
… and related C6-analogues, as well exploring the bioluminescent properties of a novel C6-thio analogue of D-luciferin. The novel analogue was targeted to provide an alternative or complementary substrate for bioluminescence imaging, that would allow for bioluminescence to be applied in systems …
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An investigation of bioluminescent microbial transport in porus media
… through porous media. A system incorporating bioluminescent bacteria, as a visual indicator of both microbial transport and activity, was coupled with a transparent porous medium that allowed the bioluminescent bacteria to be directly visualized as they were transported through a model aquifer …
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Engineering, overexpression, purification and analytical applications of recombinant bioluminescent reporters.
… there are no analytical studies using GLuc as a bioluminescent reporter. Beginning with GLuc cDNA, a plasmid encoding a biotin acceptor peptide-GLuc fusion protein was constructed. This allowed overexpression in E. coli and purification by avidin affinity chromatography. The light emission of …
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Development of Bioluminescent Biosensors Monitoring Activity of the Merlin Tumour Suppressor
All solid tumours share the ability to circumvent or overcome contact inhibition of proliferation (CIP), the process by which cell-cell contacts engage signalling to stop proliferation. Merlin, an ERM-like protein, plays a crucial role in CIP and is inactivated in a wide variety of cancers, …
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The HV1 proton channel of Lingulodinium polyedrum localizes to the bioluminescent scintillon
… (H<sub>V</sub>1) across the vacuole membrane of bioluminescent dinoflagellates and conducts protons into specialized luminescence compartments (scintillons), thus causing the pH drop that triggers the light flash. RNA-Seq data from several luminescent dinoflagellate species provided candidate …
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Characterization of a novel bioluminescent reporter protein for the non-invasive preclinical imaging of viral infection
… this enduring threat [3,4]. The integration of bioluminescent reporter genes into viral genomes presents a cutting-edge approach in understanding how a virus propagates throughout the host. This allows for researchers to non-invasively track the infection's progression from start to finish in …
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The Bioluminescent Reaction in Gonyaulax Polyedra: A System for Studying the Biochemical Aspects of Diurnal Rhythms
Made available in DSpace on 2014-12-05T19:37:44Z (GMT). No. of bitstreams: 1 6104262.pdf: 3446593 bytes, checksum: aa3c090b7bb0c4a4233fb2f94cd7d406 (MD5) Previous issue date: 1961
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DEVELOPMENT OF AN IN VIVO MULTITASKING BIOLUMINESCENT REPORTER GENE PLATFORM FOR THE PRECLINICAL EVALUATION OF RNA THERAPEUTICS
… in vitro reporter assays with in vivo bioluminescent reporter mouse models, this work enables non-invasive, longitudinal monitoring of key biological pathways involved in pato-physiological mechanisms including inflammation, oxidative stress, cell-cycle regulation, and immunoregulatory …
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Visualisation of bioluminescent Vibrio midae SY9 K811 in juvenile Haliotis midae abalone using non-invasive bioluminescence imagining
… Vibrio midae SY9 was genetically labelled with a bioluminescent plasmid (pKluxCat) containing the lux operon from Photorhabdus luminescens. The plasmid was modified by the addition of the Tn9 chloramphenicol acetyltransferase (cat) gene to enable selection of Vibrio transconjugants. The bacterial …
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CHARACTERIZING THE ROLE OF PUTATIVE VIRULENCE GENES ASSOCIATED WITH INFECTION, COLONIZATION AND PERSISTENCE OF SALMONELLA ENTERITIDIS IN CHICKEN USING A BIOLUMINESCENT REPORTER
… after oral gavage in day-old chicken, using the bioluminescent imaging (BLI) technique. SEn was genetically engineered to continuously produce light (490nm) using the light-emitting module called lux operon. This technique has been widely used in various bacteria enabling live imaging in mouse …
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Shining light on food microbiology; applications of luciferase-tagged microorganisms in the food industry
… for the emission of light can be cloned from one bioluminescent microorganism into one that is not bioluminescent. The light emitted can be monitored and quantified and will provide information on the metabolic activity, quantity and location of cells in a particular environment, in real-time. The …
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Luminescence-Based MicroRNA Detection Methods
… which will address these above problems. Bioluminescent labels are promising alternatives to current methods of miRNA detection. Bioluminescent labels are relatively small, similar in size to fluorescent proteins, and they emit very intense signals upon binding to their substrate. …
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Combination Chemotherapy and In vivo Modeling of BRCA-deficient, High-grade Serous Ovarian Cancer
… impact of BRCA status on therapeutic efficacy, a bioluminescent, BRCA-deficient xenograft model was developed. The model demonstrated characteristics resembling the disease clinically, including high disseminative tumor pattern, ascites production, and platinum sensitivity. Bioluminescent imaging …
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Bioluminescence in dinoflagellates – diversity, molecular phylogeny and field ecology
… molecular tools to investigate the identity of bioluminescent species, the genetic basis, diversity and functional regulation of bioluminescence, and the distribution of marine bioluminescent dinoflagellate populations.<br/><br/>Using “universal” and taxon specific PCR primers designed to …
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Development of in vivo tumour models for non-invasive proof-of-principle investigation of novel therapeutic agents. Engineering and characterisation of bioluminescent cell reporter systems for in vivo analysis of anti-cancer therapy pharmacodynamics.
… of cancer cells to express fluorescent or bioluminescent proteins, either endogenously or under the control of specific gene promoters, and their detection by noninvasive optical imaging has the potential to improve preclinical drug development. In this study, a panel of colorectal cancer …
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Vision and Bioluminescence in Cephalopods
… light environment mediates the visibility of bioluminescent camouflage and signals. My dissertation examines how the midwater light environment affects the ecology and evolution of vision and bioluminescence through an examination of cephalopods, a highly visual group that exhibits a broad …
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THE EVOLUTION OF BEETLE BIOLUMINESCENCE
… The study utilises two enzymes implicated in the bioluminescent reaction of fireflies and glow-worms; the protein luciferase and the luciferin regenerating enzyme (LRE). A range of parallagous genes for both enzymes were identified in bioluminescent and non-bioluminescent taxa using degenerate …
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Novel strategies to prevent and treat experimental pneumococcal disease
… progression. Real-time photonic imaging of bioluminescent bacteria offers significant advantages over conventional methods for monitoring and combating bacterial disease in animals. Not only does this approach reduce the time and costs associated with such experiments, but also it …
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MiniFAST: A Fast and Sensitive Microscope for in vivo Neural Imaging
… temporal resolution to match neuron spiking, or bioluminescent indicators which can eliminate autofluorescence and photobleaching that occurs in fluorescent indicators. Despite their potential, miniaturized microscopes are not in use with these indicators likely due to their inability to image at …
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The evolution and specialized metabolism of beetle bioluminescence
… light in a process known as bioluminescence. The bioluminescent systems of beetles, natively used for the purposes of mating communication and/or an aposematic warning signal, are now well understood and have been widely applied in biotechnology and biomedical research. There have been …
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