Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"bioluminescence resonance energy transfer"”.
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Application of fluorescence resonance energy transfer to study syndecan-3 signaling on the surface of neural cells
… complexes at the cell surface using fluorescence resonance energy transfer (FRET) and bioluminescence resonance energy transfer (BRET). Ligand binding leads to dimerization of syndecan-3 at the cell surface. The dimerized syndecan-3 colocalizes with actin in the filopodia of cells. Lysine 383 in …
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Identification de déterminants de la relation structure-activité du ligand Elabela liant le récepteur APJ par comparaison entre différentes espèces de mammifères
… de différentes espèces par approche de « Bioluminescence Resonance Energy Transfer » (BRET). Cette comparaison entre six espèces nous a poussé à étudier plus en détail le récepteur APJ de mouton puisque son profil signalétique suite à la liaison d’ELA-32 humain est fortement altéré en …
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The Bio-Nano Interface: Investigating the Roles of Nanoparticle Morphology, Microstructure, Optoelectronic Properties, and Surface Chemistry for Biological Applications
… microstructures (QRs), 2. their use in bioluminescence resonance energy transfer (BRET) systems with firefly luciferase (Ppy) enzymes, and 3. the tuning of the surface chemistry and functionality of QRs and core/alloy magnetic nanoparticles (NPs) for other biological applications. In …
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Computational and experimental investigation of G protein-coupled receptor signalling mechanisms
… underlying signalling mechanisms of GPCRs. Bioluminescence Resonance Energy Transfer (BRET)-based techniques were used to probe the underlying signalling mechanisms of the Calcitonin Receptor-Like Receptor (CLR). Results revealed a previously unknown interaction between CLR, G protein …
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Biased Constitutive Activity in the Uveal Melanoma Oncogene CYSLTR2 is Unique in CYSLTR2 Germline and Pan-Cancer Human Variome
… only poorly recruits β-arrestin as shown by a bioluminescence resonance energy transfer 2 (BRET2) based β-arrestin recruitment assay. Using a modified Slack-Hall operational model, we quantified the constitutive activity for both pathways and conclude that CysLTR2-L129Q displays profound …
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Biochemical Studies of the ER Resident Kinase PERK and Implications for Local Translational Control
… investigate the dynamics of these interactions bioluminescence resonance energy transfer (BRET) based biosensors were developed, enabling the interaction between PERK and eIF2α to be monitored in real-time in live cells. Mutating the kinase insert loop supported the in vitro binding assays by …
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Characterization of Rimonabant effects on G protein activity
… experimental approaches (i.e., GTPγS binding, bioluminescence Resonance Energy Transfer (BRET), electrophysiological recordings). In particular, we first evaluated the effects of SR on basal and agonist-stimulated [35S]GTPγS binding in systems containing CB1, GABAB and D2 receptor populations …
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Nanoparticle Biofunctionalization for Self-Assembly and Energy Transfer Applications
… This work takes advantage of both phase transfer routes to functionalize gold nanoparticles (AuNPs), quantum dots (QDs), and quantum rods (QRs) with DNA and proteins for self-assembly, energy transfer and drug delivery applications.</p> <p>In the first project, we explored the ability to …
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Lokalisation, Regulation und Interaktionen muriner DNA-Replikationsproteine
… Mit der BRET (Biolumineszenz-Resonanzenergie-Transfer)-Technik konnten direkte Interaktionen zwischen Orc2 & Orc3, Orc2 & Orc4, Orc2 & Orc5, Orc4 & Orc6, Plk1 & Orc2 und Plk1 & Dbf4 gezeigt werden. Zusätzlich wurden die Auswirkungen von Histon-Hyperacetylierung und der Depletion von …