Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"bioluminescence imaging"”.
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Bioluminescence Imaging Strategies for Tissue Engineering Applications
… and temporal manner prior to implantation. Bioluminescence imaging is a technique that has been utilized to monitor cell viability and gene expression in various in vivo applications. However, it has never been applied in an in vitro setting for the specific purpose of evaluating a …
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Real-Time Bioluminescence Imaging Of Salmonella-Neoplastic Cell Interactions
… activated by NF-κB. The reporter permitted imaging of both degradation of the NF-κB negative inhibitor IκBα and its resynthesis, which is dependent on NF-κB activation, following stimulus with Salmonella. The host kinase, NME3, was identified in the screen as a specific modulator of NF-κB. …
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Dynamic Characterization of the IKK:κBα:NFκB Negative Feedback Loop Using Real-Time Bioluminescence Imaging
… conclusion, our studies using dynamic, real-time bioluminescence imaging have demonstrated the utility of employing bioluminescent reporters alongside traditional biochemical assays, <em>in silico</em> modeling, and cell/molecular biology techniques to rigorously interrogate how diverse stimuli …
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Visualisation of bioluminescent Vibrio midae SY9 K811 in juvenile Haliotis midae abalone using non-invasive bioluminescence imagining
Bioluminescence imaging (BLI) is a powerful technique that enables real time monitoring of various processes such as gene regulation, host-pathogen interaction and distribution of bacterial cells in vivo. Most of these processes however have only been monitored in murine models or mammalian tissue …
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Investigating the effects of CSF-1R and PI3Kdelta inhibition on a breast cancer bone metastasis model
… activity leading to decreased osteolysis. Using bioluminescence imaging and μCT, we identified that GW2580 and ABT-869 significantly decreased osteolytic lesions and tumor growth in MDA-MB-231 bone metastases. Also, GW2580 increased bone mass in normal C57BL/6 mice, validating the effect of this …
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Changes of breast cancer growth and metastasis in murine models modulated by an aromatase inhibitor, a low calcium diet or a high fat diet
… metastases were monitored in live animals via Bioluminescence Imaging (BLI). Effects of ovariectomy and letrozole on body estradiol levels were examined. Tumor nodules on lungs stained with India ink were counted. Furthermore, tumor grown in lungs was analyzed via H&E staining and proliferative …
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Development of Hif-1Α/Hif-1Β Heterodimerization Inhibitors Using A Novel Bioluminescence Reporter Assay System For In Vitro High Throughput Screening and In Vivo Imaging
… therapy. To this end, a novel approach for imaging HIF-1α/β heterodimerization <em>in vitro </em>and<em> in vivo </em>was developed in this study<em>.</em> Using this screening platform, we identified a promising lead candidate and further chemically derivatized the lead candidate to assess …
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EPAC1 AS A THERAPEUTIC TARGET FOR PANCREATIC DUCTAL ADENOCARCINOMA
… of MIA PaCa-2 as determined by in vivo bioluminescence imaging and histological evaluation of the number of metastatic foci in the liver. Mechanistically, FACS-based and protein fractionation assays showed that EPAC1 facilitates activation and trafficking of integrin β1, both of which …
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Effects of Penfluridol On Integrin-Fak Signaling and Tumor Cell Killing In Combination With Oncolytic Hsv In Glioblastoma
… Penfluridol and oHSV<em>. </em>Survival data and bioluminescence imaging to evaluate virus replication was collected to examine the efficacy of the combination treatment<em>.</em> Overall, virus replication was not inhibited by Penfluridol treatment <em>in vivo</em>. Furthermore, mice treated with …
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The effect of dietary genistein on breast cancer metastasis
… three investigations were conducted: 1) to apply bioluminescence imaging (BLI) techniques to monitor the tumor progression in vivo of the two dietary groups; 2) to determine the effect of genistein on the tumor burden of the India ink-stained lungs, one of the common metastatic sites; 3) to …
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In vivo multi-modal imaging approaches for cancer, stem and immune cells
Introduction: Molecular imaging allows for non-invasive longitudinal visualization of cellular functions in vivo. This area of research has provided better understanding of fundamental molecular and biochemical processes, enabled optimization of pre-clinical and clinical assessments for new …
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Syntheses of luciferins and their bioluminescent evaluation
… an alternative or complementary substrate for bioluminescence imaging, that would allow for bioluminescence to be applied in systems where traditional D-luciferin application was limited. In the first part of the thesis, D-luciferin and a C6-amino analogue of D-luciferin, D-aminoluciferin, were …
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Understanding and engineering photoresponses in Arabidopsis thaliana
… effect upon circadian rhythms of luciferase bioluminescence but is sufficient to induce loss of robustness in rhythms of chlorophyll fluorescence, indicating that phototropins are most likely indirectly involved in the regulation of circadian rhythms via the chloroplast and not directly …
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Multimodal Imaging of Protein-Based Biomaterials for Delivering Chemo-Agent Cargo for Glioblastoma Treatment in a Murine Model
… across treatment groups, with bimodal in vivo imaging employed for evaluation. Bioluminescence imaging (BLI) tracked tumor cell activity and progression, while magnetic resonance imaging (MRI) measured end-stage tumor volume.</p> <p>Preliminary results indicated that F-TRAP-DOX exhibited …
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Characterization of Fasting-Induced p21 Expression and Protection of Intestinal Stem Cells
… various forms of stress, we generated knock-in imaging reporter mice that express firefly luciferase (<italic>FLuc</italic>) under the control of the endogenous p21 promoter. I demonstrated that FLuc expression and bioluminescence detection mirrored endogenous p21 protein levels and promoter …
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A Novel Mechanism of Enhanced Susceptibility to Bacterial Pneumonia in Influenza-infected Hosts
… the pneumococcal load in the lungs via in vivo bioluminescence imaging. Ampicillin treatment cured mice with mild pneumonia but was ineffective against severely pneumonic mice, despite effective bacterial killing. This treatment failure makes it crucial to explore immunmodulation approaches that …
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Investigating the utility of blood borne oncological biomarkers in solid tumours: glioma and melanoma
… test measures complementing radiological imaging have been occasioned by the invasive nature of malignant tumour tissue biopsy, the need for serial evaluation of tumour burden during therapy, and the need for prognostication. In a series of five studies (four clinical studies and one …