Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 83 for “"biological assays"”.
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Liquid-on-liquid mixing for slide-based biological assays
Many parallel biochemical assays rely on thin aqueous films to spread a reactant solution over a wide area decorated with multiple distinct substrates. In this asymmetric, microfluidic geometry, diffusion limits the transport of reactants to substrates. Chemical equilibrium, a requirement for …
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Microsystems platforms for array-based single-cell biological assays
… regarding a variety of fundamental biological processes. Though this in vitro approach has been fruitful, for surveying topics including cell cycle effects3' 8, pro-survival4, 7, 9, 10 and apoptotic2' 11 signaling networks, gene regulationl2' 13, and stress dynamicsl4' 15 (among …
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A microfabricated dielectrophoretic trapping array for cell-based biological assays
… traps for holding single cells and performing assays on them. The traps use the phenomenon of dielectrophoresis-the force on polarizable bodies in a non-uniform electric field-to make potential energy wells. These potential energy wells are electrically switchable, arrayable, and amenable to …
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A General Purpose Programmable Microfluidic Platform for Screening and Optimization of Biological Assays
… as on-chip droplet storage for long incubation assays, fluid waste due to large dead volumes, lack of programmable control over individual assay droplets, etc.
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Appraisal and validation of rapid, integrated chemical and biological assays of environmental quality
… both the extent of contamination and the biological effects they give rise to. This research is based on a tiered system, which commences with conventional analytical chemistry (gas chromatography), followed by the development, evaluation and application of rapid and simple immunochemical …
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Surface functionalisation of encoded SU-8 microparticles and their uses in multiplexed suspension biological assays
… developed for various multiplexed suspension biological assays. These microparticles, which are manufactured using photolithography of a commercial epoxy-based negative photoresist (SU-8), contain micrometre-sized diffractive elements and can encode millions of unique codes. In this thesis, …
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Versatile Biological Sample Preparation Platform using Microfluidic Cell Sorting Device
Biological assays for various biological samples are often limited by the low purity of target cells/particles due to the presence of significant host background. A spiral inertial microfluidic cell sorting device can be used to separate cells/particles based on their sizes without any specific …
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The Estimation of Vitamin D2, Vitamin D3, and Their 25-Hydroxy Metabolites in Animal Tissues and Foods (Hplc)
… tissues and foods. Past studies have relied on biological assays which are time consuming, expensive, equivocate total antirachitic activity with vitamin D levels, and are subjective. Recent physicochemical methods have been time consuming or insensitive. A fast, sensitive, objective, and direct …
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Design and synthesis of new potential anticancer agents and high-selective A2B antagonists
… were synthesized. Some of them were submitted to biological assays, demonstrating good activity toward different solid tumour cell-lines. In the second class of compounds, the synthetic efforts were combined to computational tools in order to achieve detailed information about the …
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Preservation of Bioactive Compounds in Blueberry Fruit
… the proanthocyanidins in a number of in vitro biological assays including anticancer, antiadhesion, and antioxidant, and evaluate how bioactivity changes when blueberries are incorporated into processed food products. A novel separation method was successfully developed that isolated high …
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Toxicity and Contamination in Bear Creek Sediment: Spatial Analysis and Implications for Risk Assessment
… demonstrating the benefits of applying joint biological assays and chemical assessment methods to sediments with complex contaminant mixtures.
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Site-directed mutagenesis and molecular modeling studies of h5-HT7(a) receptor reveal important residues for binding and activation
… and site-directed mutagenesis combined with biological assays to identify which amino acids are essential for ligand binding and receptor activation. Important residues have been identified in the 7th transmembrane domain and in the second intracellular loop of the h5-HT7(a) receptor.
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Single Molecule Förster Resonance Energy Transfer and Super Resolution Force Spectroscopy Study of Elongation Factor G Evolution, Conformation, and Function
… (T48E) and a nonpolar substitution (T48V). Biological assays indicated both inhibited tRNA translocation. Single molecule Förster resonance energy transfer (smFRET) experiments determined this inhibition stems from the mutants becoming trapped in a ‘super’-compact conformation on the …
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Electrochemical Detection of Anti-Biofilm Activity Using Unnatural Amino Acid-Containing Antimicrobial Peptides
… for this purpose. Traditional methods such as biological assays are the standard by which antibiotics are judged, but they have their drawbacks, such as the lengthy test times and the costs associated with it and the reagents. Electrochemical biosensors can remedy some of those drawbacks by …
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Studies on the production, chemical composition and nutritional value of mushroom
Chemical and biological assays were performed with freeze-dried samples of the cultivated mushroom Agaricus brunnescens. Dried fruitbodies were subjected to proximate analysis, and protein, carbohydrates, fat, moisture, ash, and amino acid contents were determined. Diets containing 10% mushroom …
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Mass spectrometric analysis of chitooligosaccharides and their interaction with proteins
… Pure homologes and isomers are applied to biological assays. Chitooligosaccahrides form high-affinity non-covalent complexes with HC gp-39 (human cartilage glycoprotein of 39 kDa). The affinity of the chitooligosaccharides depends on DP, FA and the sequence of glycosamin and …
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Isolation and identification of an antimicrobial peptide with high anticancer activity and selectivity, QUB-2584 from the skin secretion of Litoria caerulea
… the peptide. Finally, QUB-2584 was tested by biological assays, it has shown significant antimicrobial activities against S. aureus, E. coli, and C. albicans with MIC at 2, 8, and 32 μM, respectively. There was great anticancer activity of it against the cell line U251MG especially at …
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A bioactive peptide from the skin secretion of the Chinese bamboo odorous frog, Odorrana versabilis
… was synthesised by SPPS and used to conduct many biological assays.<br/><br/>QUB-1678 displayed strong smooth muscle activity on the two kinds of tissues tested, namely the rat bladder and uterus, with EC50 values of 2.413×10-7 M and 3.161×10-8 M, respectively. Meanwhile, no significant …
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Synthetic Investigation of Natural Products Causing Dopaminergic Neurodegeneration
… was undertaken to confirm its structure and biological activity. The natural product was produced from methacrolein and Roche ester starting materials that were elaborated to their respective fragments, a vinyl iodide and a Weinreb amide. These fragments were coupled to form SV-6 and the …
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High-Resolution Dynamic Mapping Of Histone-Dna Interactions In A Nucleosome Through The Use And Development Of Novel Optical Trapping Techniques
… of the unzipping method to apply to new biological assays and a novel microfluidic flow cell compatible with single molecule optical trapping experiments. These developments expand the role that optical trapping plays in the study of biological questions.
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