Global ETD Search
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Showing 1 to 6 of 6 for “"biolayer interferometry (BLI)"”.
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WDR5 Network Analysis Using Ensemble Approaches
… to characterize WDR5's interactions. We utilized biolayer interferometry (BLI) and surface plasmon resonance (SPR) to calculate the association and dissociation rate constants. Furthermore, we used fluorescence polarization (FP) to calculate the equilibrium dissociation constants. After …
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TOWARD STRUCTURAL INSIGHTS INTO THE RECOGNITION OF H3K27 TRIMETHYLATION BY CANONICAL PRC1
… heterogeneity of canonical and variant assemblies remain poorly understood. In this study, all PRC1 cores were reconstituted in vitro and purified as homogeneous complexes, enabling a direct comparison of their biochemical and structural properties. Ubiquitination assays revealed intrinsic …
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Characterisation of Alloantibody-Human Leukocyte Antigen Interactions to Improve Immunological Risk Assessment in Solid Organ Transplantation
… of human monoclonal antibodies, whilst biolayer interferometry (BLI) was used for real-time quantification of alloantibody-HLA kinetics. Outputs from each immunoassay were found to be dependent on antibody concentration, [Ab], where the degree of CDC was proportional to the antibody’s …
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Simultaneous Delivery of CRISPR/Cas Complexes and Donor DNA Using Cell-Penetrating Peptide-Adaptors
… cargos escape efficiently.</p> <p>Here, we use biolayer interferometry (BLI) and confocal microscopy to show Ca<sup>2+-</sup>dependent binding and delivery of CRISPR complexes to the nuclei of cells. Donor DNA sequences were also delivered to the nucleus of the cell. The donor DNA contained a …
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Assessment of potential antibacterial drug targets
… information about the substrate-binding site. Biolayer interferometry (BLI) and high-throughput (HTP) assay protocols were developed and applied. Despite testing around 22,000 compounds no inhibitors or suitable hits were found. This suggests <i>Bp</i>Ddl is a challenging target and a different …
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Determination and Dissection of DNA-binding Specificity for the Thermus thermophilus HB8 Transcriptional Regulator TTHB099
<p>Transcription factors (TFs) have been extensively researched in certain well-studied organisms but far less so in others. Following the whole-genome sequencing of a new organism, TFs are typically identified through their homology with related proteins in other organisms. However, recent …