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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"biolayer interferometry"”.
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Novel Applications of Biolayer Interferometry to Investigate Molecular Interactions
… results of this study demonstrate novel use of biolayer interferometry to immobilize whole Sf6 bacteriophage onto the biosensor to measure the binding to its outer membrane host receptor protein OmpA. The use of BLI also demonstrates the technology as a probe to predict and verify binding …
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WDR5 Network Analysis Using Ensemble Approaches
… to characterize WDR5's interactions. We utilized biolayer interferometry (BLI) and surface plasmon resonance (SPR) to calculate the association and dissociation rate constants. Furthermore, we used fluorescence polarization (FP) to calculate the equilibrium dissociation constants. After …
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Eph-Ephrin Signaling and the Role of EFN-4 in Caenorhabditis Elegans Nervous Systems Development
… during embryonic development. We show via biolayer interferometry that VAB-1 binds with high-affinity to EFN-4. Furthermore, EFN-4 binds with promiscuity to both the canoncial VAB-1 Eph receptor in <em>C. elegans </em>and additionally to at least one other binding partner the L1CAM (L1 Cell …
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TOWARD STRUCTURAL INSIGHTS INTO THE RECOGNITION OF H3K27 TRIMETHYLATION BY CANONICAL PRC1
… carrying the H3K27me3 modification. In parallel, biolayer interferometry (BLI) measurements revealed a markedly increased binding affinity of cPRC1.2 for H3K27me3-modified nucleosomes compared to unmodified substrates. Together, these approaches support a model in which recognition of H3K27me3 …
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Investigating the Molecular Mechanisms of SNED1 Fiber Assembly in the Extracellular Matrix
… for its fibrillar assembly. In addition, using biolayer interferometry, we demonstrated that SNED1 could directly bind to collagen I, which is the first experimentally validated binding partner of SNED1. I also examined the role of integrins in SNED1 assembly. To do this, I generated constructs …
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Characterisation of Alloantibody-Human Leukocyte Antigen Interactions to Improve Immunological Risk Assessment in Solid Organ Transplantation
… of human monoclonal antibodies, whilst biolayer interferometry (BLI) was used for real-time quantification of alloantibody-HLA kinetics. Outputs from each immunoassay were found to be dependent on antibody concentration, [Ab], where the degree of CDC was proportional to the antibody’s …
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Isolation and characterisation of novel DNA aptamers against Mycobacterium tuberculosis biomarkers: new tools for tuberculosis diagnostics
… linked oligonucleotide assays (ELONAs) and biolayer interferometry. One aptamer bound with high affinity to ESAT-6 while one aptamer bound with low affinity to LAM. The use of aptamers as capture agents for detecting biomarkers in biological specimens thus appears to be a viable option for …
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Development and Characterization of B7-H3-Targeted Radioimmunotherapies For The Treatment of Solid Tumors
… anti-B7-H3 antibody (MIL33B), validated through biolayer interferometry and ELISA. AF594-labeled MIL33B showed membrane-associated staining of tumor cells natively expressing or induced to overexpress B7-H3, displacement by unlabeled MIL33B, and no staining in knockout cell lines and isotype …
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Simultaneous Delivery of CRISPR/Cas Complexes and Donor DNA Using Cell-Penetrating Peptide-Adaptors
… cargos escape efficiently.</p> <p>Here, we use biolayer interferometry (BLI) and confocal microscopy to show Ca<sup>2+-</sup>dependent binding and delivery of CRISPR complexes to the nuclei of cells. Donor DNA sequences were also delivered to the nucleus of the cell. The donor DNA contained a …
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Targeting the Rho1 GTPase signalling pathway in <i>Aspergillus fumigatus</i>
… fragment-based small molecule screening by BLI (Biolayer interferometry) to investigate the ligandability of <i>Af</i>Rho1. Using X-ray crystallography, the structure of <i>Af</i>Rho1 was solved and a complex structure with one of the hit fragments was obtained. The complex structure showed that …
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Assessment of potential antibacterial drug targets
… information about the substrate-binding site. Biolayer interferometry (BLI) and high-throughput (HTP) assay protocols were developed and applied. Despite testing around 22,000 compounds no inhibitors or suitable hits were found. This suggests <i>Bp</i>Ddl is a challenging target and a different …
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Characterising the conformational dynamics of β₁-adrenergic receptor activation and G protein coupling
… the association of G proteins with β₁AR in biolayer interferometry assays, relative to previously characterised pre-active and inactive states. These data therefore extend our understanding of the functional states populated in solution by the β₁AR. The impact of G protein coupling on the …
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Determination and Dissection of DNA-binding Specificity for the Thermus thermophilus HB8 Transcriptional Regulator TTHB099
<p>Transcription factors (TFs) have been extensively researched in certain well-studied organisms but far less so in others. Following the whole-genome sequencing of a new organism, TFs are typically identified through their homology with related proteins in other organisms. However, recent …