Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 194 for “"biochemical characterization"”.
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Biochemical characterization of respiratory chain flavoproteins
… The protein was expressed, purified, biochemically characterized and used for in vitro screening of inhibitors. Several compounds were found to block activity of the protein and one even inhibited respiration in whole cells. SQRs (H2S + Q → S0 + QH2) are ubiquitous enzymes that can …
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Structural and biochemical characterization of LINC complexes
… these alternative binding modes. Additional biochemical characterization showed that SUN trimers can bind up to three different KASH peptides simultaneously, adding an unexpected layer of complexity to LINC complexes. A hallmark of SUN proteins is the elongated coiled-coil domain that …
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Biochemical Characterization of Glycan Assembly Pathway Enzymes
… is quite incomplete. This thesis describes a biochemical approach that may be used to provide greater insight into both glycan structure elucidation as well as the structural determinants of substrate specificity for these glycanbiosynthesizing enzymes. Phosphoglycosyl transferases (PGTs) …
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The Biochemical Characterization of Drosophila melanogaster RecQ4 Helicase
… RecQ4 from a baculovirus expression system. Biochemical characterization of the helicase, ATP hydrolysis, annealing, and binding activities of the enzyme has been performed, using native and non-native gel electrophoresis and thin layer chromatography, among other techniques. These reveal …
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Identification and Biochemical Characterization of Ghrelin O-Acyltransferase (GOAT)
… tissues. To further characterize GOAT function biochemically, I have developed a robust in vitro assay using membranes from insect cells infected with baculovirus encoding recombinant mouse GOAT. GOAT-containing membranes catalyze the transfer of [3H]octanoyl from [3H]octanoyl CoA to recombinant …
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Tobacco Phospholipase D β1: Molecular Cloning and Biochemical Characterization
Transgenic tobacco plants were developed containing a partial PLD clone in antisense orientation. The PLD isoform targeted by the insertion was identified. A PLD clone was isolated from a cDNA library using the partial PLD as a probe: Nt10B1 shares 92% identity with PLDβ1 from tomato but lacks the …
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Cloning, Purification, and Biochemical Characterization of Human Prolyl Endopeptidase
<p>Eurygaster integriceps Puton, common name sunn Pest, is one of the primary sources of wheat crop wastes in North Africa, Middle East, and Eastern Europe. It feeds by injecting the wheat grain with an enzyme characterized as prolyl endoprotease (spPEP) that breaks down Gluten, the wheat’s main …
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BIOCHEMICAL CHARACTERIZATION OF ETHANOL MODIFIED PHOSPHOLIPIDS IN SACCHAROMYCES CEREVISIAE
PHOSPHATIDYLETHANOL (PETH) IS REGARDED AS AN ABNORMAL LIPID IN CELLULAR MEMBRANE, UNIQUELY FORMED FROM PHOSPHATIDYLCHOLINE VIA PHOSPHOLIPASE D-CATALYZED TRANSPHOSPHATIDYLATION IN THE PRESENCE OF ETHANOL. IT HAS A DISTINCTLY DIFFERENT CHEMICAL STRUCTURE COMPARED WITH OTHER PHOSPHOLIPIDS AND MAY …
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Biochemical characterization of C-family heme copper oxygen reductase
… the C-family HCOs. This thesis is focused on the biochemical characterization of C-family HCO from Vibrio cholerae. Structure analysis, sequence analysis, molecular dynamics (MD) simulations and site-directed mutagenesis revealed that the C-family HCOs utilize only a single proton channel …
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The in vitro selection and biochemical characterization of metalloDNAzymes
DNAzymes are strands of catalytic DNA. First discovered in 1994, they have proved themselves capable of catalyzing many different types of reactions with significant rate enhancements. Because they often require divalent metal-ion cofactors, DNAzymes have readily been developed into metal-ion …
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Cloning, sequencing, and biochemical characterization of endo-N-acylneuraminidase
… proteolysis. In addition to sequence analysis, biochemical characterization indicates that the molecule is a dimer of identical 102 kDa polypeptide chains. Immunoblot analysis of SDS-PAGE fractionated Endo-N indicates that no post-transcriptional modifications are necessary for expression of …
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Structural and biochemical characterization of RNA polymerase II transcription
… elongation factor (NELF). Prior structural and biochemical studies provide specific mechanisms for stabilization of paused Pol II by NELF. However, cellular data suggests that NELF can accompany actively elongating Pol II into the gene body, indicating that NELF may be able to associate with pol …
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Biochemical Characterization of Normal Navicular Bone Flexor Surface Cartilage
… of the flexor surface of the navicular bone is biochemically similar to hyaline articular cartilage, but differs from previous descriptions of fibrocartilage. Further studies are needed to determine types and proportions of collagen types of the flexor surface of the normal navicular bone. These …
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Partial Molecular and Biochemical Characterization of a Sandhoff Mouse Model
Sandhoff Disease (SD) is a rare genetic disorder which results in progressive neurodegeneration. SD is caused by a build-up of GM2 Gangliosides, a lipid which is stored in the lysosome of neurons. It can occur in infantile, juvenile and adult forms; in the severe, infantile form, death occurs by …
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STRUCTURAL AND BIOCHEMICAL CHARACTERIZATION OF DEUBIQUITINATING ENZYMES, SST2 AND SSEL
Associated molecule with SH3 domain of molecule (STAM), or AMSH, is a
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Alpha-galactosidase from Lactobacillus salivarius: Isolation, purification, and biochemical characterization
In this study the enzyme $\alpha$-galactosidase ($\alpha$-D-galactoside-galactohydrolase, EC 3.2.122) has been isolated and characterized from the soluble intracellular fraction of Lactobacillus salivarius. Growth on galactose, raffinose, melibiose and lactose produced the highest levels of …
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Discovery and biochemical characterization of RNA interference in budding yeast
RNA interference (RNAi) is a eukaryotic pathway for the post-transcriptional regulation of gene expression. In the simplest form of RNAi, a double-stranded RNA (dsRNA) trigger is converted into small-RNA duplexes by the Dicer enzyme. These duplexes are then loaded into the effector protein …
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Biochemical Characterization of Arabidopsis Enzymes Involved in Inositol Pyrophosphate Biosynthesis
… described. This work aims to characterize the biochemical activity of the kinase domain (KD) of the Arabidopsis plant diphosphoinositol pentakisphosphate kinase enzymes (AtVIP1 and AtVIP2), and elucidate the biosynthesis pathway of inositol pyrophosphates in plants. Our data indicate that …
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