Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 92 for “"biocatalysts"”.
-
Characterizing and Engineering Thioesterases as Biocatalysts for Macrocyclization
… the use of TEs as in vitro macrocyclization biocatalysts, enabling the chemoenzymatic synthesis of numerous macrocycles. In the context of the existing literature on TEs, we proposed two models to provide a rational framework for understanding TE-mediated macrocyclization. The research …
-
Bioelectrochemical production of graphene oxide using bacteria as biocatalysts
… at standard conditions, and using microbes as biocatalysts. The BEGO process consists of a single-chamber microbial electrosynthesis cell (MES) that uses a graphite rod anode and a cathode (carbon cloth or stainless steel) to drive redox reactions. This MES can be operated at low voltage in a …
-
Development of oxygenase biocatalysts for the production of synthetically useful cis-diol compounds
The cis¬-dihydrodiol compounds, produced by the highly regio- and stereo-selective Rieske non-heme iron oxygenases, such as toluene dioxygenase (TDO), are versatile synthons for chiral, asymmetric synthesis. The cis-dihydroxylation of ortho- and meta-substituted phenols by TDO produce cyclohexenone …
-
OPPORTUNITIES AND CHALLENGES OF BIOCATALYSIS IN PHARMACEUTICAL SCIENCES: DEVELOPING TAILORED BIOCATALYSTS FOR SYNTHETIC APPLICATIONS
… research focuses on the development of tailored biocatalysts, specifically amine transaminases (ATAs), for the synthesis of pharmaceutically relevant compounds. To overcome typical limitations of wild-type enzymes, such as insufficient operational stability and narrow substrate scope, two …
-
Exploiting alkaloid biosynthesis in Madagascar periwinkle to obtain natural product derivatives and new biocatalysts
Plant alkaloid biosynthesis produces many natural products with medicinal value. For example, vinblastine and vincristine from Catharanthus roseus monoterpene indole alkaloid biosynthesis, and camptothecin derivatives from Ophiorrhiza pumila quinoline alkaloid biosynthesis, are anticancer agents …
-
Fundamental phenomenological description and experimental optimization of gel stabilized biocatalysts in a two-phase system
The lipase from Candida rugosa is immobilized in calcium-alginate hydrogel before been placed in hexane in order to catalyse the esterification of butanol and propionic acid. The consequence of the acidification of the aqueous phase lead to the evolution of the calcium-alginate network to an …
-
NOVEL SUSTAINABLE STRATEGIES FOR THE SYNTHESIS OF ACTIVE PHARMACEUTICAL INGREDIENTS (APIS) AND BIOACTIVE MOLECULES THROUGH THE EXPLOITATION OF ISOLATED ENZYMES AS BIOCATALYSTS
… interest, by combining the ability of various biocatalysts alongside traditional organic synthetic protocols. Both commercially available and in-house expressed, recombinant enzymes were considered, exploiting their activities as isolated proteins to produce scaffolds of pharmaceutical interest …
-
Advancing biocatalytic technologies for degradation of micropollutants in drinking water
… enzymes, referred in this dissertation as biocatalysts, offer targeted and rapid destruction of micropollutants such as perchlorate. This targeted destruction of perchlorate even in the presence of orders of magnitude higher concentrations of co-competing compounds offers a distinct …
-
Charakterisierung und Optimierung gel-stabilisierter Zwei-Phasen-Systeme zur stereospezifischen Reduktion hydrophober Verbindungen mit der Carbonyl-Reduktase aus Candida parapsilosis
… high substrate and reaction specificity of many biocatalysts. Interesting starting compounds are often not or only slightly soluble in water. The employment of an organic solvent offers a convenient alternative to the aqueous system. Use of a gel-stabilized two-phase-system could avoid an …
-
Desenvolvimento de suportes enzimáticos por polimerização simultânea em suspensão e emulsão
… polymerizations, aiming at producing enzymatic biocatalysts. Initially, different reaction strategies were adopted in order to increase the specific area and porosity of the produced particles. Thus, it was observed that, by reducing the duration of the suspension step, core-shell particles of …
-
The crystal structure of a mutant nitrile hydratase
Nitrile hydratases can be used as industrial biocatalysts. They catalyze the conversion of nitriles to their corresponding amides. These industrial biocatalysts have recently gained attention due to the economic production of industrial commodities such as acrylamide and nicotinamide (Thomas et …
-
Ganzzellbiotransformationen mit rekombinanten Escherichia coli zur Synthese chiraler Alkohole
… Escherichia coli cells were applied as biocatalysts for the reduction of ketones. By the expression of suitable alcohol dehydrogenases (adh) both enantiomeres of chiral alcohols can be produced. The syntheses of enantiopure (R)-3-hydroxybutyrate and (S)-3-hydroxybutyrate were carried out …
-
Encapsulation of Achiral Mn(Salen) Complexes Into Cytochrome C Peroxidase: Spectroscopic Characterizations and Epoxidation Studies
… other protein scaffolds to produce a library of biocatalysts to perform various organic transformations.
-
Synergy of protein and genome engineering for fuels and chemicals production
… compounds. Therefore, technologies that use biocatalysts have emerged as a promising foundation to build a green, sustainable bioeconomy. However, to derive value-added compounds from biomass, significant engineering work has to be performed to optimize naturally occurring organisms with …
-
Übertragung der Produktion hydrophober Substanzen mit immobilisierten Biokatalysatoren in den technischen Labormaßstab
Biocatalysts play an essential role as reaction catalyst for synthesizing organic compounds due to their substrate-, stereo- and regiospecificity. While their natural environment is predominately the aqueous phase, many economically interesting reactants are poorly soluble in water. However, the …
-
Characterisation of the hydantoin-hydrolysing activity of pseudomonas putida strain RUKM3s and development of biocatalyst for amino acid production
… and operational parameters of the immobilised biocatalysts were evaluated. Stabilisation of biocatalysts by immobilisation revealed that among five methods of immobilisation used, covalent coupling to Eupergit® C provided the most suitable biocatalyst formulation of the RUKM3s enzymes. A model …
-
Capsid catalysis : de novo enzymes on viral proteins
… M13 semisynthetic enzyme platform complements biocatalysts with characteristics of heterogeneous catalysis, yielding high-surface area, thermostable biochemical structures readily adaptable to reactions in myriad solvents. As the viral structure ensures semisynthetic enzymes remain linked to …
-
Investigating chlorite dismutase biocatalyst reuse using columns
… method. This process would require reuse of the biocatalysts to reduce costs for continuously operated water treatment systems. To study the potential for biocatalyst reuse, experiments were conducted on a chlorite dismutase biocatalyst modified with a strep tag; this reuse system allows the …
-
Designing and optimizing tandem bio- and chemo-catalyzed cascade reactions to access industrially relevant chemicals in mild, aqueous conditions
… catalysts in conjunction with whole cell biocatalysts. The studies are organized by type of abiotic catalyst and include examples featuring electrocatalysts, photocatalysts, and chemocatalysts. Throughout the review we take note of the challenges faced when interfacing these two strategies …
Page 1 of 5