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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 56 for “"biocatalyst"”.
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Investigating chlorite dismutase biocatalyst reuse using columns
… method. This process would require reuse of the biocatalysts to reduce costs for continuously operated water treatment systems. To study the potential for biocatalyst reuse, experiments were conducted on a chlorite dismutase biocatalyst modified with a strep tag; this reuse system allows the …
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Human lactate dehydrogenase heterogeneous biocatalyst for efficient anticancer drug screening
L'abstract è presente nell'allegato / the abstract is in the attachment
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Engineering of an efficient and enantioselective biocatalyst for the preparation of chiral pharmaceutical intermediates
… engineering of an efficient and enantioselective biocatalyst via direct evolution and genetic engineering for the enantioselective hydroxylation of non-activated carbon atom, a useful but challenging reaction for the synthesis of chiral pharmaceutical intermediates. Our target enzyme is the novel …
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The Laccase from Micromonospora sp.044 30-1 as a biocatalyst for synthesis of antioxidant compounds
… to water. The varied uses of laccase as a biocatalyst can be attributed to its ability to produce a free radical from a suitable substrate. Of importance to the pharmaceutical, chemical, and industrial sectors, are the laccase-catalysed reactions providing means for the synthesis of dimeric …
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Use of a Purple Non-Sulphur Bacterium, Rhodopseudomonas palustris, as a Biocatalyst for Hydrogen Production from Glycerol
… bacterium, Rhodopseudomonas palustris, as a biocatalyst for hydrogen production, from the waste of biodiesel manufacturing, crude glycerol. The goal of this project was to understand the fundamentals relevant to scaling up the process and developing an off the shelf product. The first …
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Characterisation of the hydantoin-hydrolysing activity of pseudomonas putida strain RUKM3s and development of biocatalyst for amino acid production
… stabilised by immobilisation, and applied as a biocatalyst for production of amino acids from 5-mono-substituted hydantoin substrates. Experiments were devised to optimise conditions for the production of RUKM3s biomass with high levels of hydantoin hydrolysing activity, and to evaluate …
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Mechanistic understanding of microbial desulfurization
… gained interest due to the ability of certain biocatalysts to desulfurize compounds that are recalcitrant to the currently employed hydrodesulfurization (HDS) technology. Two of the major obstacles to commercialization of BDS are mass transport limitations and the inability to maintain …
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Enantioseparation using a counter-current bioreactor
… action, a high partition of the biocatalyst to the stationary phase was required in order to retain the biocatalyst in the coil, with differing partitions of substrates and products between the stationary phase (SP) and mobile phase (MP) so that these could be separated. Aqueous …
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Elektroenzymatische Synthese mit ionischen Flüssigkeiten
… liquids on the activity and stability of the biocatalyst, on the solubility of the used substrates, on the conductivity of the reaction medium as well as on the involved electrochemical reaction step were investigated. These examples showed that not only the conductivity of the reaction medium …
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2,3-Butanediol production from whey permeate using immobilized cell systems
… the fermentation: a $\beta$-galactosidase-active biocatalyst consisting of non-viable cells of Kluyveromyces fragilis and immobilized cells of Bacillus polymyxa.
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Reactor design for the hydroxylation of n-octane using a cyp153a6-based biocatalytic system expressed in Escherichia coli
… monooxygenase, CYP153A6, enzymatic system. The biocatalyst was expressed in Escherichia coli BL21(DE3) by using a pET28b-PFR1500 plasmid encoding the complete operon from Mycobacterium sp. HXN-1500 which included the ferrodoxin reductase (FdR) and ferrodoxin (Fdx) redox partner proteins. …
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FROM AGRI-FOOD WASTE TO HIGH-VALUE COMPOUNDS VIA GREEN TECHNOLOGY APPROACHES
… process intensifica:on, enzyme engineering, and biocatalyst immobiliza:on to maximize efficiency, scalability, and environmental sustainability. First, a co-immobilized mul:-enzyme system combining a commercial α-rhamnosidase and an extremophilic β-glycosidase was implemented for the efficient …
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DEVELOPMENT OF SUSTAINABLE AND EFFICIENT TAILOR-MADE PROCESSES FOR PHARMA AND FOOD APPLICATIONS
… In some cases, a combination of more than one biocatalyst was used in the same protocol to perform further modifications of the high value chemicals obtained. Biocatalysts were employed in different physical forms (immobilised or free), as isolated enzyme or whole cells. The best approach was …
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Genetic characterization of Rhodococcus rhodochrous ATCC BAA-870 with emphasis on nitrile hydrolysing enzymes
… rhodochrous ATCC BAA-870 potentially a versatile biocatalyst for the synthesis of a broad range of compounds with amide and carboxylic acid groups that can be derived from structurally related nitrile precursors. The selectivity of biocatalysts allows for high product yields and better atom …
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Engineering functional biomaterials to advance environmental and resource sustainability
… novel protein-based biosorbents and enzyme-based biocatalysts for resource recovery, separation, and conversion applications. The research focuses on three key objectives: (1) designing a novel magnetic nanoplatform for protein immobilization; (2) evaluating this nanoplatform by fabricating …
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Cell-Free Biosystems Comprised of Synthetic Enzymatic Pathways: Development of Building Blocks, Immobilization of Enzymes, Stabilization of Cascade Enzymes, and Generation of Hydrogen
… and stabilize them for long periods to decrease biocatalyst costs. Thermophilic recombinant enzymes as building blocks were discovered and developed: fructose 1,6-bisphosphatase (FBP) from Thermotoga maritime, phosphoglucose isomerase (PGI) from Clostridium thermocellum, triose phosphate …
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Beyond bottlenecks: expression of complementary enzymes and permeabilization of cell membranes to improve performance of CYP153A6 in Escherichia coli whole cell biocatalysis
… of these enzymes in vitro is hampered by low biocatalyst stability, inhibitory upstream processing costs, and the need for costly cofactor supplementation. In vivo operation can overcome these issues, but brings its own set of limitations. This thesis presents research on the in vivo …
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Integration of composite nanomaterials into anode and cathode designs
… conductive material and Bilirrubin oxidase, the biocatalyst, immobilized silica-gel. The composite nanomaterials and the immobilization techniques utilized in this research would help as departing stage to engineer electrodes designs that meet the criteria required for optimum biofuel cells.'
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The Termite Digestome: Understanding the Digestive Physiology Involved in Lignocellulosic Biomass Degradation
… digestive physiology and discover potential biocatalysts that can aid in the degradation of lignocellulosic biomass. Various protein characterization and gene expression methods were used throughout this research in order to accomplish these objectives. The results of this dissertation …
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Streamlining the development of biophotoelectrodes: from rational design to rapid screening
… an abundant source of scalable and sustainable biocatalysts. Interfacing these microorganisms with a biophotoelectrode has emerged as a promising platform to harness solar energy for power generation or chemical production. Nonetheless, the development of biophotoelectrodes has been a …
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