Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"bioanalysis"”.
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Fluorescence Resonance Energy Transfer (FRET) Based Sensors for Bioanalysis
… resonance energy transfer (FRET) for bioanalysis. Chapter 3 describes the use of FRET between donor fluorophores and acceptor labeled murine macrophage cells. FRET microscopy was used to determine whether the donor molecules truly permeate through the cell membrane or only adsorb to …
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Magnetic forces for surface-based bioanalysis in microfluidic devices
… solid-supports in a continuous flow system for bioanalysis. 2) The use of diamagnetic repulsion forces for label-free on-chip particle handling.For the continuous flow system, a magnetic particle type was selected from eight commercially available brands of particles and characterised using on …
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Ion-pair based Molecular Imprinted Polymers for bioanalysis and sustainable use of organocatalysts
This thesis presents the development and application of ion-pair functional monomers as a new concept in stoichiometric molecular imprinting, as well as a proof-of-concept application of urea and thiourea based monomers in the development of an assay of thyroxine in patient blood samples. …
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Single-molecule detection of molecular beacons generated from LDR on thermoplastic microfluidic device for bioanalysis
Current clinical techniques for nucleic acid detection and analysis often involve PCR, lacking adequate specificity or sensitivity to meet the stringent requirements in certain applications. This research aims to develop an innovative molecular assay and the associated hardware to rapidly signal …
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Direct analysis of dried blood spot samples
… support high sample throughput quantitative bioanalysis in a regulated drug development environment. An initial literature review, coupled with proof of concept testing of the most prominent direct analysis techniques coupled to mass spectrometers (MS), resulted in direct elution (direct …
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Subcellular Fractionation Methods for the Quantification of Endogenous Protein in Human Liver Using Targeted Proteomics
… sensitivity, UPLC-MRM is widely used in sample bioanalysis for pharmacokinetics. Recently, interest into human endogenous protein quantification using targeted quantitative proteomics has increased. These quantification measurements are useful in many aspects of drug discovery and development, …
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New phosphorescence based probes and techniques for the analysis of cellular oxygen and respiration
… and respiration is on the cutting edge of bioanalysis, including studies of cell metabolism, bioenergetics, mitochondrial function and drug toxicity. This thesis presents the development and evaluation of new luminescent probes and techniques for intracellular O2 sensing and imaging. A new …
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Characteristics and Performance of Novel Squarylium Dyes as Fluorescent Probes for the Analysis of Proteins and Viruses by CE-LIF
… design of more selective and sensitive dyes for bioanalysis.
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Design and Application of Hollow Silica Microspheres for Density-based Bioseparations
… and pathogens for improved detection and bioanalysis of infectious diseases.
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Design and application of hollow silica microspheres for density-based bioseparations
… and pathogens for improved detection and bioanalysis of infectious diseases.
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Preparation and characterization of Staphylococcal protein A adsorbents for high performance affinity chromatography
… Performance Affinity Chromatography (HPAC) to bioanalysis are addressed. The initial study demonstrates the quantitative determination of IgG from serum using HPAC. Columns were prepared by immobilizing Protein A from Staphylococcus aureus on 10 m diol-bonded silica. Immunoglobulin …
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Bio-interfaced Nanolaminate Surface-enhanced Raman Spectroscopy Substrates
… SERS has attracted tremendous interest in bioanalysis over the last two decades due to minimal sample preparation, non-invasive measurement without water background interference, and multiplexing capability from rich chemical information of narrow Raman bands. Nevertheless, significant …
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Development of capillary electrophoretic-based techniques to analyze doxorubicin in tissues, cells and subcellular fractions.
… (MEKC) is becoming a popular method in bioanalysis due to its high sensitivity and separation efficiency, small sample size requirements, simple sample preparation procedures, versatility in sampling and short separation times. The goal of this thesis is to take advantage of these …
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Quantitative analysis of co-enzyme a in a plant tissues and cancer biomarkers in urine samples by using capillary electrophoresis and liquid chromatography-mass spectrometry
<p>"Despite continuous effort and progress in cancer detection and therapy, cancer remains one of our greatest health concerns because of its low survival rate and rapid incidence increase. One reason for this is the late detection and therefore pre-cancer diagnosis is crucial. In pre-cancer …
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Single molecule methods for clinical biomarker quantification
… Despite extensive demand for improved bioanalysis platforms, state of the art methods remain dependent on incredibly complex and time consuming techniques which exhibit insufficient sensitivity and minimal capacity for multiplexing. This thesis aims to address these limitations through …
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An Investigation into the Use of Dried Blood Spot Analysis in Pharmacokinetic Studies
The ethical and practical issues of obtaining a blood sample pose a significant challenge to performing pharmacokinetic studies in children, infants and neonates. Dried blood spot analysis, based on the collection of a micro blood sample has potential to overcome these difficulties. There are at …
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Miniature bioanalytical assays utilizing mechanical actuation of microspheres
… has the potential to enable high sensitivity bioanalysis with the ease-of-use profile required for decentralized settings. In the first part of this thesis, we describe a framework for predicting bead-analyte capture over a range of bioanalytes to aid in design across applications. We apply …
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