Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 22 for “"binding study"”.
-
Binding study of nickel complex to protein by equilibrium dialysis
… 15-pentaene, was chosen as the complex in this study based on previous studies that showed it to be the most active complex among a series of square planar nickel complexes in promoting DNA oxidation. This complex was synthesized and its structure was proven by electrospray mass spectroscopy, …
-
Determination of Calcium Binding to Beta-Casein at Various Ph Values Using the Calcium Murexide Method
Calcium binding to casein affects the formation of casein micelles and the behavior of the casein proteins during processing. Since calcium binding is pH dependent, it is important to understand the binding properties of the non-micellar caseins with calcium ions under the influence of pH. The …
-
Investigating antimicrobial resistance mechanisms in Neisseria gonorrhoeae using peptide probes
… systems and consequently challenging to study and target with drugs. The transcriptional regulator (MtrR) of the efflux pump, however, is a soluble protein and therefore more amenable to study and drug target validation investigations. This thesis serves to investigate the hypothesis that …
-
Small Molecules Binding to Serpins
… were closely monitored during fluorescent binding studies, the ACT-CHY along with the AT:TRY interaction. Four different conformational variants of each system were studied including the native, cleaved, latent and complex forms. Three different fluorescent dyes were used to identify the …
-
A STUDY OF THE THERMODYNAMICS OF ANION BINDING TO HUMAN SERUM TRANSFERRIN
… environment of the ion centers of the protein by binding to specific anion sites. The binding of these anions (thiocyanate, perchlorate, adenosinetriphosphate, pyrophosphate, and chloride) is shown to occur pairwise with high positive cooperativity, an observation unique to nonstereospecific …
-
Semisynthetic cytochrome c site-67 substitutions
… and 50 mV respectively. Both analogs had similar binding constants for imidazole, increased approximately 10-fold over the native protein and (Hse-65)cytochrome $c$. In a cyanide binding study, however, (Hse-65, p-F-Phe-67)cytochrome $c$ had a 3- to 5-fold lower binding constant than the other …
-
Mechanistic insights into the cancer cell cytotoxicity and blood stability of the garlic compound ajoene
… 20 μM). A spectrophotometric and proteomic binding study demonstrated S-thiolation between ajoene and the cysteine 93 residue in the -subunit of haemoglobin which may explain the observed blood instability. A biotinylated ajoene analogue was designed and synthesised to identify ajoene's …
-
Protein/Peptide Recognition Modules in Cellular Signaling and HIV Pathogenesis
… (Src homology 2) domains, PTB (phosphotyrosine binding) domains, and SH3 (Src homology 3) domains. The SH2 domains recognize tyrosylphosphorylated peptides using a binding mode that is conserved among the SH2 family members. In particular, the structure of the SH-PTP2 SH2 domain (described in …
-
Advances in molecular scale electronics: Synthesis and testing of nanoscale wires and devices
… ethynylenes in the areas of cluster and surface binding study, STM patterning, and organic LEDs.
-
Purification and Characterization of the Prostate Cancer-Associated Antigen: 7E11-C5
… protein (PSP)) were unable to block the binding of MAb 7E11-C5 to its target antigen. Western blot analysis demonstrated that there was no similarity between the 7E11-C5 antigen and these three antigens. The 7E11-C5 antigen was purified 400-fold from LNCaP xenograft tissue extract by …
-
Metals and the conformation of fibrin
… the substrate (f-D-dimer). A differential zinc binding study determined that the potentiation of activity by zinc was due to a zinc-substrate rather than a zinc-enzyme interaction. The binding constant for zinc to D-dimer was determined by Scatchard analysis of zinc titration data. The …
-
[Beta]-amyloid-mediated nitric oxide release from rat microglia by ligation of the integrin Mac-1
… neurotoxicity. In the present study nitric oxide release from cultured rat microglia was examined by exposing the cells to synthetic [beta]-amyloid peptides ([beta]25-35 and [beta]1-40) alone and in combination with cytokines. Assessment of microglial release of nitric oxide was …
-
Structural Characterization and <em>in vitro</em> Antiproliferative Activity of Non-Specific Lipid Transfer Protein 1 (nsLTP1) from Fennel (<em>Foeniculum vulgare</em>) Seeds
… similar features, they exhibit distinct lipid-binding specificities signifying their various biological roles that dictate further structural study. The present study determined the complete amino acid sequence, <em>in silico</em> 3D structure modeling, and <em>in vitro </em>antiproliferative …
-
Structure Activity Relationships for Intracellular Loop 2 of the 5HT1A Serotonin Receptor
… have been used as probes and the current study includes peptides P21 to P27 of ic2 (LDRYWAITDPIDYVNKRTPRPR), approaching the ic2 carboxy terminus of TMD4 two residues per 10 MER. Peptide ability to uncouple receptor from G protein was determined in agonist inhibition studies using the …
-
Structure Activity Relationships for Intracellular Loop 2 of the 5HT1A Serotonin Receptor
… have been used as probes and the current study includes peptides P21 to P27 of ic2 (LDRYWAITDPIDYVNKRTPRPR), approaching the ic2 carboxy terminus of TMD4 two residues per 10 MER. Peptide ability to uncouple receptor from G protein was determined in agonist inhibition studies using the …
-
Diverse functions of LanC-like proteins in mammalian cells
… enzymes to modify proteins/peptides. In this study, dehydroamino acids containing peptides that mimic the activation loop of Erk and Akt protein kinases were synthesized. LanCL proteins were shown to add GSH to these dehydroamino acids containing peptides. The same C-glutathionylation was also …
-
Carbon Nanotube Alignment within Macrostructures
… investigate electrical properties, a large tight-binding study of pairwise CNT contacts of different diameters and chiral angles was performed and showed that small contact angles favour inter-tube conductance with minimal backscattering. Registry effects were shown to be important only when the …
-
The synthesis and characterization of a new kind of tagged Ru-Pt bimetallic DNA binding agent
… The BAS represents the bioactive sites for binding to DNA, in this case the cis-Pt<sup>II</sup>Cl₂ moiety based on previous studies with cisplatin. The Ru-Pt bimetallic complexes [(tpy)Ru(PEt₂Ph)(BL)PtCl₂](PF₆)₂ (BL = bpm or dpp) and their precursors were designed, successfully synthesized …
-
Development of nanoplasmonic devices for biosensing applications
… two biosensing applications. The first is a drug binding study to cytochrome P450 proteins and the second is a cell adhesion imaging study. From these studies, the advantages and disadvantages of the plasmonic nanocup array are identified and a new plasmonic sensor is developed. This sensor design …
-
Dna Aptamers Selected Against Wild-Type Helix 69 Ribosomal Rna And Their Implications In Combating Antibiotic Resistance
… as an ideal target to develop ligands with high binding specificity. </p> <p> In this thesis work, DNA aptamer candidates with binding specificity for the wild-type bacterial H69 were selected. The 84-nt-long DNA aptamer (H69DNAapt18) that was identified from a DNA library with a 40-nt randomized …
Page 1 of 2