Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 58 for “"binding kinetics"”.
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Application of silicon photonic microring resonators to the study of surface bioconjugation and protein capture agent binding kinetics
… recently utilized for monitoring biomolecular binding and detection in an array-based, label-free biosensor format. First, aniline-catalyzed hydrazone ligation between surface immobilized hydrazines and aldehyde-modified antibodies is shown to be an efficient method to functionalize model …
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Analysis of heme-copper ligation, quinol activity, and ligand binding kinetics of cytochrome BO(3) quinol oxidase from E. coli
… ligand bound absorption spectra, and ligand binding kinetics. Structural and functional perterbations by the mutation of conserved residues were determined with ligand binding kinetics. Finally, construction and characterization of histidine tagged subunits I, II, and III was described.
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Combined optical trapping and single molecule fluorescence to study the force-dependent binding kinetics between filamentous actin and its partners
… nucleus. However, the effects of forces in the binding kinetics between actin filaments and actin binding proteins are unknown. This work proposes an experimental setup to study the force-dependent binding kinetics of such proteins at the single molecule level by using an instrument that …
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Immobilization Of The Anti-Thrombin DNA Aptamer: Attachment, Optimal Packing Density And Kinetics
… effect immobilization of aptamers, the analyte-binding kinetics of attached aptamers, and the optimal assay format for aptamers. These studies utilized the well-characterized anti-thrombin DNA aptamer. Investigations were conducted in order to ascertain effective methods of immobilization of the …
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Microbead-Based Biosensing in Microfluidic Devices
… and used as a fluorescent label to monitor binding to the bead. Three-dimensional finite element simulations were carried out to model the mass transfer and binding kinetics on the beads’ surfaces and within the porous beads. The theoretical predictions were critically compared and favorably …
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Hierarchical selective electrokinetic concentration : the universal next-generation biomolecule enrichment technique for molecular diagnostics
… Because of the slow mass transport and weak binding kinetics at ultralow concentration of target biomolecules, enrichment of target biomolecules plays an essential role in the detection of ultralow-abundance biomolecules. Currently, nucleic acid tests rely on enzymatic processes for target …
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Kinetics and regulation of the initial stages of cadherin-mediated adhesion
… mechanism put forth held that cadherins mediated binding through exchange of a tryptophan containing strand at the N-terminal domain. Another theory holds that there is an initial equilibrium which then is converted to the strand exchange form. These theories do not fully account for the range of …
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Development of an Enzyme-Linked ImmunoSorbent Assay (ELISA) with Enhanced Sensitivity in a Nanofluidic System
… studies were performed to evaluate the kinetics and equilibrium binding constants of biomolecules in nanofluidic channels. Binding events in the nanochannel were detected using electrical and fluorescence methods. We concluded that antibody-antigen binding constants in nanochannels were …
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Regulation of cadherin adhesion at intercellular junctions
… domain interactions, ligand or antibody binding, post translational modifications, or inside out signaling from cytoplasmic binding proteins. In this thesis, micropipette-based adhesion frequency measurements of cadherin-mediated, cell-cell binding kinetics identified a unique kinetic …
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Novel Applications of Biolayer Interferometry to Investigate Molecular Interactions
… molecular interactions to demonstrate molecular binding and to provide quantitative characterization of binding kinetics and affinities of phage-protein and DNA-protein interactions. The results of this study demonstrate novel use of biolayer interferometry to immobilize whole Sf6 bacteriophage …
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Endothelial cells and basement membrane : a co-regulatory unit for fibroblast growth factor-2 in hyperglycemic stress
… vascular disease could be related to altered kinetics of vasoactive compounds within this regulatory unit. We hypothesized that hyperglycemic stress mediates storage, release, and function of fibroblast growth factor-2 (FGF-2) through changes in interaction between endothelial cells and …
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Improved mehtods and reagents for pretargeted radioimmunotherapy of cancer
… (RIT) by decoupling the pharmacokinetics of the targeting agent and the radioisotope. In order to improve upon PRIT, we have considered variables such as treatment setting and methodology, the transport and clearance characteristics of targeting agents, and the radionuclides used …
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Novel use of a cell-penetrating peptide-adaptor system to characterize Akirin constructs and investigate activity of bacterial effector proteins
… entrapment. Calmodulin (CaM) is capable of binding to the calmodulin binding site (CBS) of cargo only in the presence of calcium. TAT-CaM can be used to characterize Akirin constructs and investigate the activity of bacterial effectors, AopP and AvrA, in the NF-kB pathway. Akirin is a small …
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The Role of Potassium in Betaine-Homocysteine Methyltransferase
… of BHMT that are involved in Hcy- and betaine-binding. These residues include Asp26 and Gly27, which along with Gly28 comprise an amino acid fingerprint that is common among Hcy S-methyltransferase proteins. All three residues of this DGG motif were substituted with a different amino acid …
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Combined experimental and modeling studies reveal new mechanisms in T cell antigen recognition
… and indicated that TCRs quickly upregulate their kinetics several magnitudes upon initial antigen recognition for antigen recapture. Additionally, OT-1 double positive thymocytes were probed by pMHC using a Biomembrane Force Probe (BFP) with different ligands under the presence of CD8, a …
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A Kinetic Study of Anti-VEGF-A Polyclonal Antibodies and Anti-VEGF-A ssDNA Aptamers
… anti-VEGF-A aptamer had the same, if not better, binding kinetics than the anti-VEGF-A polyclonal antibody, and as such offers an ideal replacement for use in in field, real-time testing assays.</p> <p>SPR revealed that both the polyclonal antibody and ssDNA aptamer bound the target antigen, …
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The expression and purification of the regulatory domain of PKG1 alpha
… cyclic nucleotides as well as its activation and binding kinetics of the enzymes can be determined. This information will help us to investigate the detailed mechanism of activation of the enzyme and to develop drugs to target these kinases better. Purification of the enzyme is started with …
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Hydrodynamic and Mass Transport Properties of Microfluidic Geometries
… geometries. The first examines the heterogeneous binding kinetics in a microbead array, where beads with different chemical functionalities are sequentially captured in a well geometry over which analyte solution is flowed. Finite element simulations identified the flow rates and microbead surface …
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Biochemical Analysis of the Protein-Protein Interactions Involved in Karyopherin-Mediated Transport Across the Nuclear Pore Complex
… among the most important missing details is the binding kinetics of almost all the transport relevant interactions, due to significant technical challenges. The aim of this work is to analyze the protein-protein interactions involved in Kap-mediated transport across the NPC, using biochemical, …
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Relative effectiveness of inhibition strategies for control of biological signaling pathways
… its target can be characterized by the binding kinetics and thermodynamics. However, the overall efficiency with which the inhibitor shuts down a pathway or process, arrests a signal, or interferes with an outcome can be quite different and is often measured in cell-based assays. Because …
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