Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"binary vector"”.
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Characterization of a minimal abiotic stress-inducible promoter in the development of a binary vector for crop protection
… (ABR1PR) and successfully incorporated it into a binary vector system which drives strong reporter transgene expression (enhanced yellow fluorescent protein, eYFP) under multiple abiotic stress conditions in transgenic tobacco (Nicotiana tabacum). The ABR1/ERF111 gene is a member of the group X …
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Characterization and Expression of Plant Defense Genes in Peach and Tomato
… strain GV3101 harboring derivatives if the binary vector pCAMBIA 2301. The binary vectors pGR-1, pChi-1, pRIP-1, and pAFP-1 carried a maize beta-glucanase, maize chitinase, iris ribosome-inactivating protein (RIP), or an antimicrobial protein gene, respectively, each driven by the CaMV 35S …
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Influenza hemagglutinin expression in Nicotiana tabacum and Nicotiana benthamiana.
… insertion into plants was developed using the binary vector, pE1802. Agrobacterium tumefaciens was transformed with the plasmid and both Nicotiana tabacum or Nicotiana benthamiana were transformed. To screen for HA3 expression, mRNA was isolated from first generation plants. Second generation …
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Targeted expression of the anti-HIV microbicide lectin griffithsin in maize and tobacco
… experimentation with different host systems, vector systems, codon optimisations and subcellular targeting. (Maclean et al., 2007; Yang et al., 2005). The latter sometimes results in unexpected locations which might reflect on an inherent property of the protein itself or specifically be …
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Cluster analysis of soybean pathogen-responsive genes and functional characterization in Arabidopsis
… were cloned into an Agrobacterium tumefaciens binary vector and transformed into Arabidopsis thaliana to determine if these genes have a cross-species effect on enhancing disease resistance. This ongoing research project provides genes to be used for promoter analysis and identifies genes …
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Generation of Transgenic <i>Medicago Sativa</i> Overexpressing "<i>Osmotin-Chitinase</i>" Gene Chimera
… (<i>OSM-CHI</i>) gene chimera cloned into binary vector pBTEX with <i>npt</i>II as a selection marker was mobilized in <i>Agrobacterium tumefaciens</i> strain EHA105 which was employed in the transformation of hypocotyl explants of <i>Medicago</i>. Transformed calli were grown on callus …
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Isolation and Characterization of the Soybean Major Latex Homologue Msg, and Analysis of Its Pod -Specific Promoter
… The different regions were cloned into the binary vector pBI101 with a promoterless uidA (GUS) reporter gene. Fourteen deletion fragments of the 5' region were compared by particle bombardment of soybean pods and by transformation into Arabidopsis by Agrobacterium vacuum infiltration. The …
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Isolation and Characterization of the Soybean Major Latex Homologue Msg, and Analysis of Its Pod-Specific Promoter
… The different regions were cloned into the binary vector pBI101 with a promoterless uidA (GUS) reporter gene. Fourteen deletion fragments of the 5' region were compared by particle bombardment of soybean pods and by transformation into Arabidopsis by Agrobacterium vacuum infiltration. The …
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Randomized sampling and multiplier-less filtering
… develops design techniques using parametric binary process models to optimize the performance of randomized non-uniform sampling. In addition, a detailed second-order error analysis, including performance bounds and results from simulation, is presented for each type of sampling. The second …
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Genetic engineering for pest resistance in potato (Solanum tuberosum L.)
… ('Iwa') were transformed using an Agrobacterium binary vector bearing the gene encoding for avidin protein. The avidin protein is known for its insect antimetabolite properties. Both cultivars were successfully transformed with the avidin gene. Surprisingly, 'Red Rascal' showed the lowest overall …
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Characterization of Effector Genes in Acidovorax citrulli the Causing Agent of Bacteria Fruit Blotch Disease of Cucurbits
… was studied. A novel Gateway-compatible binary vector was developed for transient expression of A. citrulli type III effectors genes in planta. A set of modified vectors for marker-exchange mutagenesis in A. citrulli were constructed. The model plant species Nicotiana benthamiana was …
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Genetic Transformation of Switchgrass (Panicum Virgatum L.) with Endoglucanase Gene and Characterization of Plants with Endoglucanase Transgene
… with maize ubiquitin promoter in a modified binary vector pCambia 1305-2 and transformed into switchgrass genotype HR8 by using Agrobacterium tumefaciens. In this study, I generated five independent transgenic switchgrass lines and they were confirmed by growing on the selection agent …
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Characterization of grapevine vein clearing virus expression strategy and development of caulimovirus infectious clones
… pGVCV-1, was inserted into an Agrobacterium binary vector for delivery into plant cells. The co-agroinfiltration assay described in chapter 3 was applied, and pGVCV-1 was shown to be capable of replication and encapsidation. Furthermore, a systemic veinal chlorosis symptom was observed in …
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Expression of atrial natriuretic factor in transgenic potatoes
… six chimaeric gene fusions were cloned into the binary vector, pGA643, or its derivative. These were then introduced into potato plants via leaf disc transformation using Agrobacterium tumefaciens strain LBA4404 carrying the disarmed helper Ti-plasmid, pAL4404. About 300-400 kanamycin-resistant …