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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"bimolecular fluorescence complementation"”.
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Analyse und Visualisierung eines R2R3-MYB / bHLH Transkriptionsfaktorkomplexes aus Arabidopsis thaliana
… analyzed with new methods from the physics like fluorescence correlation spectroscopy (FCS) or fluorescence resonance energy transfer (FRET). With FCS and recombinant expressed proteins, promoter attachment sites of the transcription factor complex were identified. Simultaneous binding of both …
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Studies on the POLARIS gene of Arabidopsis
… and PLS by using both Yeast Two Hybrid and Bimolecular Fluorescence Complementation assays. In addition, the pls mutant has defective auxin transport and accumulation. The present work shows that PLS is required for correct gravitropic response, gene expression in the quiescent centre and …
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Development and Application of a Novel Assay for Rapid Identification of Cellular Prion Protein Modulators
… and modulation. The LIPS system is based on the Bimolecular Fluorescence Complementation (BiFC) technique, usually used for protein-protein interaction study, and here adapted for protein localization and quantification. LIPS was optimized for high-content screening (HCS) to identify small …
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Functional studies on receptor-type protein tyrosine phosphatases of the R3 subgroup
… contacts have been reported. Here I used a bimolecular fluorescence complementation (BiFC) assay in live cells to examine the molecular basis for the interaction of one of the R3 RPTP members, VE-PTP, with VE-cadherin, and explored the potential of others to interact with this protein. The …
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<i>Maize fine streak virus</i> (MFSV) gene expression and protein interaction
… generated by means of yeast two-hybrid (YTH) and bimolecular fluorescence complementation (BiFC). The MFSV 3 and 4 proteins strongly interacted with each other in both assays, suggesting that this interaction is likely to occur in nature. Based on the location of MFSV 4 in the genome, the size of …
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Molecular visualization and localization of ribosomal subunits interaction in cells
… fragments of yellow fluorescent protein so that bimolecular fluorescence complementation (BiFC) occurs only when the subunits join to form a 80S ribosome. With this technique I was able to visualize translation sites in Drosophila S2 cells and in transgenic flies. Translation sites are most …
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The functional characterization of a novel protein, Liat1
… Using a combination of immunocytochemistry, bimolecular fluorescence complementation, and biochemical techniques, we found that Liat1 undergoes LLPS in the dense fibrillar compartment (DFC) of the nucleolus. Mutational analysis revealed that a low complexity, poly-lysine region contained in …
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Influenza Viral Protein Interaction Detecting Methods Development and A.M1-host Interplay Network Investigation
… subunits interaction. In addition to two protein complementation assays, Split luciferse complementation assay (SLCA), and Bimolecular Fluorescence Complementation (BiFC), LUMIER (luminescence-based mammalian interactome mapping) assay were also developed and used in this study. We demonstrated …
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Discovery of a Novel Signaling Circuit Coordinating Drosophila Metabolic Status and Apoptosis
… we developed an assay based on bimolecular fluorescence complementation (BiFC) to monitor the oligomerization of Dronc in S2 cells, a crucial step in its activation. The sensitivity of this assay has been validated with several apoptotic stimuli. A future whole-genome screen …
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Functional Characterization of Four Xanthomonas euvesicatoria Type III Effectors
… disease assay. The interaction is confirmed by bimolecular fluorescence complementation, co-immunoprecipitation and split luciferase assay. Intriguingly, we also revealed that XopX also interacts with multiple Xe T3Es including AvrBS2, XopN, XopB, and XopD in the co-IP assay. The virulent and …
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TRAF Regulation of Caspase-2-Dependent Apoptosis in Response to DNA Damage
… of caspase-2. Specifically, we utilized the Bimolecular Fluorescence Complementation technique, wherein complementary halves of the Venus fluorophore are fused to caspase-2: when caspase-2 dimerizes, the non-fluorescent halves fold into a functional Venus fluorophore. We combined this …
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Lokalisation, Regulation und Interaktionen muriner DNA-Replikationsproteine
… ist. Mit der erst kürzlich entwickelten BiFC- (bimolecular fluorescence complementation) Technik konnte sowohl die cytoplasmatische als auch die nukleäre Lokalisation der Interaktion zwischen Orc2 und Orc3 gezeigt werden. FLIP- (fluorescence loss in photobleaching-) Studien mit BiFC-positiven …
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Functional Analysis of Insl5 and Insl6 Genes and Verification of Interactions between Pelota and its Putative Interacting Proteins.
… interagierenden Proteine zu bestimmen, wurde ein bimolecular fluorescence complementation assay (BiFC) durchgeführt. Die mittels dieser Untersuchung erhaltenen Ergebnisse zeigen, dass CDK2AP1, EIF3G und SRPX je einen Proteinkomplex mit PELO bilden, die am Cytoskelett lokalisiert sind.