Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"bicuculline"”.
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Determination of Dissociation Constants for GABAA Receptor Antagonists using Spontaneously Active Neuronal Networks in vitro
… µM (mean ± SD, n=6). At 10, 20, 40 and 80 µM bicuculline, the muscimol IC50 values were shifted to 4.3 ± 1.8 µM (n=6), 6.8 ± 1.7 µM (n=6), 19.3 ± 3.54 µM (n=10) and 43.5 µM (n=2), respectively (mean ± SD). Muscimol titration in the presence of 10, 20, 40 µM of gabazine resulted in IC50s values …
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Mechanisms of inhibition and neuronal integration for signal processing in the primary auditory cortex of the Mongolian gerbil (Meriones unguiculatus)
… application of the GABAA-receptor antagonist bicuculline in the auditory cortex of the lightly anaesthetized gerbil (Meriones unguiculatus). Bicuculline generally increased the spontaneous neuronal activity and enhanced and prolonged onset responses to sound. Application of bicuculline often …
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Using Hi-Spots to investigate in vitro network dysfunction in Cysteine String Protein ? knockout mice
… stimulated by the inhibitory receptor antagonist bicuculline. The validity of its more in vivo-like organization, not observed in more conventional dissociated cultures, is evidenced by a glutamate toxicity resistance in the Hi-Spot cultures. Further, the thesis describes the optimisations to the …
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The role of the GABAergic system in the production of ultrasonic vocalization in rats /
… apphcation ofGABA antagonists (picrotoxin or bicuculline) will reduce caibachol-induced vocalization. A total of sixty rats were implanted with stainless steel guide cannulae in the AH/POA area. After recovery, animals were locally pretreated with 1) GABA (l-40ng), 2) picrotoxin (1 .5^g) or …
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Age-Dependent Effects Of Chronic GABAA Receptor Blockade In Barrel Cortex
… containing the GABAA receptor antagonist bicuculline were placed over barrel cortex for a 4-day period in young (postnatal days 8 - 12) and adult rats. In the cortex of young, but not adult rats, the chronic blockade of GABAA receptors resulted in substantial tissue loss and neuron loss. …
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Cellular Effects of Cerebral Ischaemia In Vitro’. Cerebroprotective Actions of GABAmimetic agents
… inhibited ischaemic glutamate efflux in a bicuculline-insensitive manner whilst pentobarbitone did not, indicating that clomethiazolc may exert its neuroprotective action by direct activation of the GABAa receptor. Electrophysiological studies using cells stably transfected with al:pl:Y2L …
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Evaluation of Zinc Toxicity Using Neuronal Networks on Microelectrode Arrays: Response Quantification and Entry Pathway Analysis
… pretreated with the GABAA receptor antagonists bicuculline (40 mM) and picrotoxin (1 mM) lacked the initial excitation phase. This suggests that zinc-induced excitation may be mediated by interfering with GABA inhibition. Partial network protection was achieved by stopping spontaneous activity …
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The Pedunculopontine Nucleus: A Potential Site of Respiratory Modulation During Muscle Contraction and Hypoxia
… significance in that microinjections of bicuculline, a GABAA receptor antagonist, into the PPN resulted in significant increases in respiratory activity. The second set of studies demonstrated importance of the integrity of pathways that synapse in the PPN to the respiratory responses …
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Ovarian Hormone Modulation of Learning Strategy Preferences: A Role for Hippocampal Disinhibition
… infusions of the GABA A antagonist bicuculline failed to shift strategy use from response to place in estrous rats (Chapter 2), while intrahippocampal infusions of the GABAA agonist muscimol caused proestrous rats to shift from a place to a response strategy preference (Chapter 3). …
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Male-specific mechanisms in a murine model of nociplastic pain
… injection of the GABAA receptor antagonist bicuculline or the GABAB receptor antagonist CGP 52432. In females, spinal GABAB receptor inhibition, but not GABAA receptor inhibition, followed by vibration stimulation was able to trigger a transition to the nociplastic pain state. However, this …
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Male-specific mechanisms in a murine model of nociplastic pain
… injection of the GABAA receptor antagonist bicuculline or the GABAB receptor antagonist CGP 52432. In females, spinal GABAB receptor inhibition, but not GABAA receptor inhibition, followed by vibration stimulation was able to trigger a transition to the nociplastic pain state. However, this …
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Unique applications of cultured neuronal networks in pharmacology, toxicology, and basic neuroscience
… network activity that resembled those induced by bicuculline, a known epileptogenic. Further work determined that TMPP produces its effects on network activity through a competitive inhibition of the GABAA receptor. This demonstrates that neuronal networks can provide rapid, reliable warning of …
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The synthesis and pharmacology of gamma-aminobutyric acid receptor mimetics
… 3H-muscimol, and the GABA antagonist, 3H-bicuculline methiodide. Recently, it has been suggested that 1,2,3,6 Tetrahydropyridine-4-carboxylic acid (Isoguvacine) is a potent GABA receptor agonist. It has also been reported to act as a mixed agonist/antagonist at the GABA-benzodiazepine …
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Excitotoxicity, oxidative stress and neuroprotection in cerebellar granule neurones
… 3-nitropropionic acid, hydrogen peroxide, bicuculline and 4-aminopyridine. Preconditioning with 100µM N-methyl-D-aspartate at 8 DIV was effective in protecting against the neurotoxic effects of glutamate or 3-nitropropionic acid applied 24 hours after the commencement of preconditioning, …
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The synthesis and biochemical and pharmacological evaluation of gamma-aminobutyric acid antagonists
… also with GABA antagonists using (3H)-bicuculline methobromide and (3H)-dihydropicrotoxinin. Utilising these methods a number of agonists and antagonists have been examined to evaluate their ability to displace the stereospecific binding. The synthesised GABA antagonists have also been …
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GABA as a Putative Neurotransmitter in the Optic Lobes of the Crab HEMIGRAPSUS NUDUS
… surface receptor binding as picrotoxin and bicuculline did not appear to effect uptake. Similar biochemical experiments showed little glial . involvement. 2,4-diaminobutyrate ( L-DABA ), shown in other studies to preferentially inhibit uptake by neurons but not glia, matched the degree of …
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Regulation of Luteinizing Hormone and Catecholamine Release
… muscimol, or either the GABA^ antagonist, bicuculline, or the putative GABAg antagonist, 5-aminovalerate. Additional experiments tested the effects of the GABA drugs on LH release in ovariectomized, hormonally untreated rats and in response to exogenous LHRH. The LH surge induced by …
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Spatio-temporal structure of thalamically evoked activity in the barrel cortex
… seconds by such stimuli. During application of bicuculline, ensemble activity was blocked, instead epileptiform activity appeared with ten-fold higher amplitudes in the LFP. The observations that ensemble activity had a much smaller amplitude than epileptiform activity, that the on- and offsets …
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The interactive and individual effects of orexin A and neurotensin in the brain on spontaneous physical activity.
… area. The acute effect is also observed with bicuculline-mediated stimulation of neurons in the rostrolateral hypothalamus. It is likely that there are other peptide signaling systems which are downstream of orexin receptor activation in the rostrolateral hypothalamus that are responsible for …
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The effects of rapid stretch injury on rat neocortical cultures
… action potentials are blocked by 20 uM bicuculline and 3 mM kyneuri nic acid, the frequency of subthreshold depolarizations is measured to estimate overall neuronal network activity. The frequency of spontaneous subthreshold depolarizations is not significantly different in injured and …
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