Global ETD Search
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Showing 1 to 4 of 4 for “"bialaphos"”.
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Investigation of the biosynthesis of the phosphonate antibiotics bialaphos and dehydrophos
Phosphonate and phosphinate natural products possess a range of biological activities as a result of their ability to mimic phosphate esters or tetrahedral intermediates formed in enzymatic reactions involved in carboxyl group metabolism. The widespread use in medicine and agriculture of these …
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Investigations into the use of maize streak virus as a gene vector
… contained three different versions of a bar (bialaphos resistance) expression cassette in place of the viral movement and coat protein genes were used to generate transformed maize cell lines. A high proportion of these contained MSV-based episomes at high copy number. However, embryogenic …
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Advancing plant transformation and CRISPR/Cas editing: Strategies for recalcitrant maize
… transformation efficiency, the inefficacy of bialaphos selection in suppressing non-transgenic regenerants was observed due to shorten regeneration step. In response, we developed a new T-DNA binary vectors with neomycin phosphotransferase II (NptII) selection and RUBY reporter for efficient …
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B-cyclin/CDK Regulation of Mitotic Spindle Assembly through Phosphorylation of Kinesin-5 Motors in the Budding Yeast, <italic>Saccharomyces cerevisiae</italic>
… to phleomycin, hygromycin B, nourseothricin and bialaphos. Finally, in the process of generating the new plasmids, I have determined several errors in existing publicly available sequences for several commonly used yeast plasmids. Using updated plasmid sequences, I constructed pRS plasmid …