Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 45 for “"beta-sheet"”.
-
Ensemble modeling of [beta]-sheet proteins
Our ability to characterize protein structure and dynamics is vastly outpaced by the speed of modern genetic sequencing, creating a growing divide between our knowledge of biological sequence and structure. Structural modeling algorithms offer the hope to bridge this gap through computational …
-
A Survey of Beta-Sheet Folding: WW Domains With Zinc Fingers and Membrane Peptides
… can dictate the folding dynamics of this model beta-sheet protein. We then report on a synthetic zinc-finger peptide used to bridge the gap between experimental and theoretical protein folding research. To complete our work we summarize ongoing research on the membrane interactions of a small …
-
Molecular mechanism of Abeta recognition and neuroprotection by the glycoconjugate beta-sheet-breaker peptide Ac-LPFFD-Th
… To this regard, peptide-based inhibitors of Abeta aggregation have been widely investigated with a particular emphasis to those derived from original amyloid sequences. The experimental work described in the present PhD Thesis aims at outlining the molecular mechanisms underlying the …
-
Conformational and Orientational Dynamics of Semi-Rigid Macromolecules in Shear Flow
… undergoes a conformational transition to a $\beta$-sheet. In this study, the stability of the secondary structure of poly-L-lysine in solutions subjected to shear flow, at temperatures below the $\alpha$-helix to $\beta$-sheet transition temperature, were examined using Raman spectroscopy and …
-
Design and synthesis of constrained dipeptide units for use as β-sheet promoters
The study of factors promoting beta-sheet formation has recently gained interest due to the suspected involvement of beta-sheets in brain degenerative diseases such as Alzheimer's disease (AD), Creutzfeldt-Jacob disease (CJD) and bovine spongiform encephalopathy. Understanding beta-sheet formation …
-
A Novel Statistical Potential for Protein Beta-Sheets Prediction
… prediction problems, a fundamental problem is Beta-sheets structure prediction. Though more than 85% of experimentally solved proteins contain Beta-sheet structures, limited methods have been found to rapidly and accurately predict the folded conformations. In this study, we proposed a novel …
-
Insights Into the Dynamic Structure and Inhibitory Mechanism of Plasminogen Activator Inhibitor Type 1
… PAI-1 exhibited more partial insertion into the beta-sheet A and that beta-sheet A was more accessible to the mobile RCL than was evident from the crystal structure of 14-1B. These properties suggested that PAI-1 be grouped in a novel sub-class of serpins exhibiting partially-inserted RCLs and …
-
Mechanical behavior and microstructure of self-assembling oligopeptide gels
… that self-assemble into a solution of beta-sheet filaments. These filaments can aggregate to form a gel suitable for culture of various cell types. Examples of such self-assembling peptides are RAD16-I, RAD16-II, and KLD-12. One limitation of self-assembling peptide hydrogels is their …
-
Model membrane interactions with ions and peptides at the air/water interface
… with two different peptides B18 and Amyloid beta (1-40) peptide were investigated. B18 is a synthetic peptide that binds to lipid membranes that leads to the membrane fusion. It was demonstrated that it adopts different structures in the aqueous solutions and in the membrane interior. It is …
-
Analysis of the thermodynamic determinants of protein fold specificity in the denatured state ensemble
… diseases are known to be related to non-specific beta sheet formation. The value of this project lies in the detailed analysis between denatured ensemble energetics and sequences, as well as between energetics and secondary structures. Correlation analysis between structure and energetic …
-
Investigation of the Role of Amide I to Amide II Ratio and Alpha Helix to Beta Sheet Ratio of Faba Bean Seeds in the Determination of Microbial Protein Synthesis and Animal Performance and Metabolism in Ruminant Livestock Systems.
… of amide I to amide II ratio, and α-helix to β-sheet ratio (α_β) on the protein quality (solubility and digestibility), metabolism, and bioavailability in dairy cows has not been established. Thus, the objective of this research was to evaluate the role of the protein-related spectral ratios on …
-
Structure-function analysis of HAMLET (human alpha-lactalbumin made lethal to tumor cells)
… which changes from a mixed alpha-helical and beta-sheet conformation to the beta-sheet rich, disease causing iso-form. Protein folding is becoming recognised as a mechanism to generate beneficial functional diversity, however. One example is alpha-lactalbumin, which by unfolding forms a …
-
Size-dependent mechanical properties of beta-structures in protein materials
… in particular, hydrogen bonds (H-bonds). Beta-structures, a class of protein folds that employ dense arrays of H-bonds, are universal in strong protein materials such as silks, amyloids, muscle fibers and virulence factors. The biological recipe for creating strong, tough materials from …
-
NMR study of the unfolding of ribonuclease A, and dynamical studies of liquids in confined geometries
… and all three $\alpha$-helices and the $\beta$-sheet of the native protein remain partially folded structures in the pressure denatured state. $\sp1$H spectra of the denatured RNase A suggest that the cold and pressure denatured states are more structured than the heat denatured state, …
-
Multiscale Modelling of Biomolecular Phase Behaviour
… I focus on delineating the role of spontaneous beta-sheet folding – prime drivers for neurodisease-linked phase transitions – in the kinetic and transport properties of condensates as a function of the abundance and strength of such transitions. Using atomistic umbrella sampling simulations I …
-
Sequence-structure correlations in the MaSp1 protein of N. clavipes dragline silk
… residues used to represent a cross-linking 7-sheet crystal node in the network within a fibril of the dragline silk thread. Understanding the behavior of this node at the molecular scale is critical for potentially bypassing strength limits at this length scale and vastly improving silk for …
-
A Fragile Native State Structure: An Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) Variant Exhibits Slow Interconversion Kinetics Between two Different Folds
… with several other bHLH-PAS proteins via the beta-sheet surfaces of its two PAS domains. The beta-sheets of PAS domains are involved in many intermolecular interactions with other proteins and natural cofactors in order to detect environmental changes in sensor PAS proteins. As part of a study …
-
Mechanism of aggregation of oxidation-mimicking mutants of human [gamma]D-crystallin
… thesis focuses on human [gamma]D crystallin, a [beta]-sheet rich, topologically complex two-domain protein that is abundant in the lens nucleus, associated with cataract by both genetic and proteomic data, and representative of the larger y-crystallin family. To mimic oxidative damage, we …
-
Structural heterogeneity in silk fibers and its effects on failure mechanics and supercontraction
… a heterogeneous structure that comprises [beta]-sheet crystals embedded in an amorphous matrix. However, it is not fully understood how the heterogeneity of silk affects its mechanical properties. First, the origin of the nanoscale heterogeneity during the Nephila Clavipes dragline silk …
-
Effects of Glycosylation on Lecithin -Cholesterol Acyltransferase
… alpha helical content of N384Q LCAT and in the beta-sheet content of N84Q LCAT by CD, compared to WT LCAT. Fluorescence measurements of the binding of the probe ANS suggested that in both mutants, the active site cavities became inaccessible with time. In conclusion, both mutants lost catalytic …
Page 1 of 3