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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 54 for “"beta-galactosidase"”.
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The Induced Synthesis and Properties of Beta-Galactosidase of Saccharomyces Fragilis
Made available in DSpace on 2014-12-05T21:45:28Z (GMT). No. of bitstreams: 1 0020863.pdf: 4404780 bytes, checksum: ede4ac7bc409c2f111529040f819bf08 (MD5) Previous issue date: 1957
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Investigating the effects of calreticulin and beta-galactosidase on microglial functions and neuronal loss
… loss were affected by calreticulin and β-galactosidase, two proteins associated with ageing, chronic inflammation, and neurodegeneration. The aggregation of amyloid-β to form oligomers and insoluble amyloid plaques in the brain is a hallmark of AD. Amyloid-β can be directly neurotoxic and …
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Beta-Galactosidase: A Possible Model Enzyme for Studying the Genetics of Enzyme Regulation in Mammalian Systems
Made available in DSpace on 2014-12-14T15:38:02Z (GMT). No. of bitstreams: 1 7624126.pdf: 3286208 bytes, checksum: 0672cacc55267684c7bf8a3730256c70 (MD5) Previous issue date: 1976
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THE EFFECT OF STRINGENT CONTROL ON THE PRODUCTION OF ENTEROTOXIN, TRYPTOPHANASE, AND BETA-GALACTOSIDASE BY ESCHERICHIA COLI IN A RECYCLING FERMENTOR
… during phases 2 and 3, while tryptophanase and (beta)-galactosidase were more negatively affected by ppGpp. An enhancing effect by glucose was found on the production of enterotoxin and (beta)-galactosidase. The nicking process of enterotoxin activation appears to be regulated by ppGpp and may be …
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The identification of chemical compounds that decrease cellular levels of toxic Huntington's disease protein through a novel cell-based assay
… assay using the enzymatic activity of E. coli [beta]-galactosidase as a reporter for Htt protein levels. I expressed either expanded (97Q) or normal (23Q) Htt fused to the [beta]-galactosidase alpha-subunit ([alpha]) in an inducible fashion in PC12 cells which also expressed the …
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Lactose Operon Expression and Plasmid Rearrangement in Rhodopseudomonas Sphaeroides (Gram Negative Bacteria)
… operon, Tn951, was examined. Very low levels of (beta)-galactosidase were observed in R. sphaeroides but mutants of this bacterium were recovered that were able to use lactose as the sole carbon source and which had elevated (beta)-galactosidase expression. The mutation allowing growth on lactose …
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Application of metabolic and biochemical engineering techniques for the enhancement of recombinant protein production in Escherichia coli
… plasmid encoded cadA-LacZ fusion protein with $\beta$-Galactosidase activity under the control of the pH-regulated promoter. This recently developed expression system has the potential for very high gene expression, of 30 to 40% of the total soluble cell protein. Three approaches are presented. …
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2,3-Butanediol production from whey permeate using immobilized cell systems
… substrate hydrolysis and the fermentation: a $\beta$-galactosidase-active biocatalyst consisting of non-viable cells of Kluyveromyces fragilis and immobilized cells of Bacillus polymyxa.
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Characterization of the 5̕ untranslated region ( 5̕ UTR) of the alcohol oxidase I (AOX I) gene in Pichia pastoris.
… 5' UTR. All constructs were assessed using the β-galactosidase activity assay to determine if various constructs led to an increase or decrease in the rate of translation. Deletions had a variable effect on β-galactosidase expression, whereas additions decreased expression but not in a …
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Physical, genetic, and biochemical analyses of mannityl opine catabolism genes from the Agrobacterium Ti plasmid pTi15955
… the transposon-induced lacZ fusion mutants by $\beta$-galactosidase activity and mannityl opine utilization patterns identified at least six genetic units associated with catabolism of these opines. Functions for catabolism of MOP and mannopinic acid (MOA) are encoded by a 16.4 kb region, whereas …
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Characterizing phenotypes of Pichia pastoris mutants that show enhanced secretion of recombinant proteins
… exhibited an unusual ability to supersecrete beta-galactosidase, due to the effects of a randomly disrupted gene by pREMI-Z. To learn more about the novel effects of the gene disruption, nine beta-galactosidase supersecreters ( bgs ) have been characterized for their phenotypes such as growth …
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BIOTRANSFORMATION AND MUTAGENICITY OF BENZIDINE
… Ames tester strain TA98, and the inductest-(beta)-galactosidase assay. The transformation products were mutagenic without requiring prior microsomal activation. The inductest-(beta)-galactosidase assay (Elespuru and Yarmolinsky, 1979) was modified extensively to increase its sensitivity for …
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An analysis of the effect of mutations in the leader region of the threonine operon of Escherichia coli on attenuation or transcription termination
… the plasmid copy number, and assaying for the $\beta$-galactosidase activity.
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Síndrome de morquio : uma revisão bibliográfica
… type A, and type B is caused by a deficiency of beta - galactosidase. This syndrome brings as a consequence ske letal disorders, muscle and heart, thus impairing the physical and motor carrier without occurring psychiatric disorders. Diagnosis is made through a queratosulfato the dosage taken by …
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A signal for retention of cytochrome P450 2C1 in the endoplasmic reticulum
… soluble cytoplasmic protein, Escherichia coli $\beta$-galactosidase, or to a secreted protein, Escherichia coli alkaline phosphatase, and the hybrid proteins were expressed in COS1 cells and cellular localization was determined by subcellular fractionation, immunolocalization and the …
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Liposome-Mediated DNA Transfer
… inch (psi), respectively. Based on the results, beta-Galactosidase activity was assayed as a measure of transfection efficiencies at 10,000 psi compared with atmospheric pressure. Three days following liposome transfections cells were assayed beta-Galactosidase activity and transfection …
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Protein -Dna Interactions Required for Regulation of the Proline Utilization Operon in Salmonella
… was analyzed by proline dehydrogenase assays and beta-galactosidase assays using a putA::MudJ fusion downstream of each of the put control region mutant. Although the mutations isolated in the put control region are defective in PutA binding in vitro, they do not show a strong defect in vivo..
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PLASMA MEMBRANE ASSOCIATED GLYCOHYDROLASES: KINETICS PARAMETER CHARACTERIZATION AND BIOLOGICAL FUNCTION
… assay) method able to measure the activity of beta-galactosidase, CBE sensitive beta-glucosidase, GBA2 beta-glucosidase, SMase and beta-hexosaminidases, enzymes working directly at the plasma membrane level using MUB derivates as substrate. Afterwards we applied this assays in order to study …
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뇌종양 동물모델에서의 인간신경줄기세포주의 종양으로의 특이적 이주에 관한 입체적 분석
… 8 9. Hematoxylin and Eosin stain 9 10. Beta-galactosidase immunofluoresecence 9 11. Statistical Analysis 10 Ⅲ. RESULTS 11 1. Tumor-tropism pattern of NSCs 11 2. Migration rate of NSCs 19 3. NSC migration studied by stereology 23 4. Histological analysis 28 5. Proliferation of NSCs in …
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