Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"beta-actin"”.
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Evaluation of the Hypothalamic Kisspeptin System Throughout the Estrous Cycle and During the Attainment of Puberty in Gilts
… of hypothalamic Kiss1, Kiss1R, ER-alpha, ER-beta and beta-actin was determined using real-time reverse transcriptase PCR. Fold changes in relative expression were determined using the Relative Expression Software Tool. Relative expression of Kiss1 was increased (P=0.006) 3.2 fold on d 1 …
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Expression and function of transglutaminase 1 in the brain
… in synaptic endings was found. In addition β-actin was found to be a substrate for this synaptic transglutaminase activity. This finding was supported with the expression of a recombinant transglutaminase 1, which was able to cross-link a small primary amine to beta-actin. A model was proposed …
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Investigating the defects of postnatal global Fli1 deletion in a mouse model
… a Fli1flox/flox mouse with CAG Cre under the beta-actin promoter. Based on what has been previously described in mice with deletion of Fli1 in specific cell types, we predicted that global postnatal deletion of Fli1 will result in systemic fibrosis, vasculopathy, and inflammation in these …
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Gain-of-Function Mouse Models to Investigate Biological Roles of Prmt6
… on an <em>ER*-Flag-PRMT6</em> fusion (under a beta-actin promoter), while the other model is based on a Cre-inducible system, generated by cloning a floxed transcriptional Stop cassette upstream a <em>Flag-PRMT6</em> (under a beta-actin promoter). Upon PRMT6 induction, both mouse models show …
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Gene Expression in Endometrial Tissues of Normal Mares and Mares With Delayed Uterine Clearance
… using the expression of two housekeeping genes, beta actin and ribosomal 18S RNA. Quantitative real-time polymerase chain reaction (QPCR) was used to evaluate gene expression of the selected genes. Results indicated that there was no statistically significant difference in the expression of any …
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Maternal Exposure to Prostaglandin E2 Affects Hippocampal Synaptic Plasticity in Mice Offspring -A Link to Autism Spectrum Disorder
… components NMDAR subunit 2A, AMPA subunit GluR1, beta-actin, and morphological characteristics such as primary dendrite length and cell soma size of pyramidal neurons in the hippocampus. I found that PGE2 exposure decreased LTP in males and I/O responses in females at higher stimulation …
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Genetic association between schizophrenia and type-2 diabetes
… cells. Two house-keeping<br/>genes (HKGs), beta-actin (ACTB) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were used as the internal controls to normalize the Ct values of the target genes. Fisher’s combining probability test was used to determine the combined p values from the …
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Differentially expressed genes in aortic cells from atherosclerosis-resistant and atherosclerosis-susceptible pigeons
… energy. Myosin light chain kinase and alpha actin were exclusively expressed by the SR SMC, whereas beta actin and collagen were dominant in the WC. Because of the compressed in vitro time frame compared with in vivo development, it was not obvious whether insufficient ATP synthesis is …
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Adeno-Associated Mediated Transduction of Sensory Epithelia Within the Guinea Pig Cochlea
… Furthermore, the CAG promoter (also called CMV-beta-actin promoter) was shown to drive gene expression specifically in hair cells while the glial fibrillary acidic protein (GFAP) promoter drove expression in the non-sensory support cells. This in vivo study was designed to determine if these …
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Proteomic Analysis of Human Mesenchymal Stem Cell Aging during serial sub-culture
… Similarly, it was found that Vimentin, Beta actin, Nucleophosmin, and Heat shock protein 70 (Nterminal frag) showed up regulation in protein expression at initial passages and down regulation at late passages while Transgelin, Prolyl 4-hydroxylase subunit alpha-2, …
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Adeno-Associated Mediated Transduction of Sensory Epithelia Within the Guinea Pig Cochlea
… Furthermore, the CAG promoter (also called CMV-beta-actin promoter) was shown to drive gene expression specifically in hair cells while the glial fibrillary acidic protein (GFAP) promoter drove expression in the non-sensory support cells. This in vivo study was designed to determine if these …
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The effect of CLAs on the expression of PKC isoforms and cell viability in breast cancer cell lines.
… using PKC isoform specific antibodies and Beta-actin, or Claudin-3 as the housekeeping protein. Results indicate that the CLA isoforms used (the 911, 1012 and a 50/50 mix of the two) cause a decrease in cell viability in both cell lines. It was observed that the activation of PKC Alpha was …
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Understanding The Visual And Auditory Defect In Ush2a Mouse Model
… mislocalization of its other USH2 interacting partners. Along with patients exhibiting phenotypes associated with homozygous mutations of USH2A, there is also a prevalence of cases involving heterozygous mutations of USH2A in combination with mutations in other USH or non-USH genes that …
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Role of Chaperonin CCT in G-protein Biosynthesis
… include cytoskeletal proteins (alpha-, and beta-actin, alpha-, beta-, and gamma-tubulin), histone deacetylases (HDAC3, Set3p, and Hos2p), cell cycle regulators (Cdc20p, and Cdc55p), and proteins possessing the seven-bladed beta-propeller WD40 domain, including the beta-subunit of the …
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Immune function alterations in Asian elephants (Elephas maximus) infected with Mycobacterium species
… (TNF)-alpha, transforming growth factor (TGF)-beta, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), and beta actin were determined. Sequence information was then utilized to design elephant-specific, quantitative, real time RT-PCR and in situ hybridization assays for measurement of cytokine …
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Interference of Toxoplasma gondii with IFN-γ-regulated gene expression of its host cell
Der obligat intrazelluläre Parasit Toxoplasma gondii ist dazu fähig in seinem Wirt eine lebenslange persistierende Infektion zu etablieren. Diese Persistenz wird durch eine ganze Reihe von Immunevasions-Strategien des Parasiten erreicht. Eine solche Strategie ist die Parasiten-vermittelte …