Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 188 for “"base-pair"”.
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Fluorescent anthracene tags for detection of base pair mismatches in DNA duplexes
… (PrA, PrB, PrC, PrD, PrE, PrF and PrG) as a DNA base analogue using automated synthesis. Melting temperature (T\(_m\)) studies of double-stranded 15-mer / 14-mer deletion sequences compared to 15-mer / 15-mer analogues demonstrated that the incorporation of anthracene serves to stabilise the …
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Direct observation of XPD helicase base-pair stepping and regulation by RPA2
… we present a singlemolecule helicase assay with base-pair sensitivity, which utilizes high-resolution optical tweezers combined with microfluidics and fluorescence microscopy to decipher how FacXPD helicase is modulated by FacRPA2. FacXPD is the archaeal homolog of yeast Rad3 and human xeroderma …
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Consequences of Oxidative DNA Damage: Base-Pair Dissociation, Singlet Oxygenation and DNA-Protein Crosslinking
… resulting from oxidatively generated damage and pairs with complementary cytidine (C) in Watson Crick base-pairing within duplex DNA. The WC-OG·C lesion, if not recognized or repaired, not only leads to G⋅C→T⋅A transversion mutations (through Hoogsteen (HG) base pairing of OG⋅A), but also renders …
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The Isolation and Characterization of Bacteriophage-T4 Base Pair Substitution Mutations Exhibiting Extremely Leaky Phenotypes
Made available in DSpace on 2014-12-09T22:04:23Z (GMT). No. of bitstreams: 1 7013382.pdf: 1290605 bytes, checksum: ccb222fe698e532df9df52b66dbc44ea (MD5) Previous issue date: 1969
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Singlet Oxygen Oxidation of Guanine, 9-Methylguanine and Guanine-Cytosine Base Pair: Dynamics and Kinetics Revealed by Parallel Gas- and Solution-Phase Experiments and Computations
… of guanine, 9-methylguanine and guanine-cytosine base pair, from the gas-phase bare ions, through hydrated clusters, to aqueous solution. Various techniques have been adapted in the work, including <sup>1</sup>O<sub>2</sub> generation and detection, guided-ion beam tandem mass spectrometry, …
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Determination of Mutation Rates in Bacteriophage T4 by Unneighborly Base Pairs and a Genetic Analysis of the Error-Prone Repair System of Bacteriophage T4
… mutation frequency of a particular base pair can be influenced by the base pair adjacent or penultimate to the measure site. This study extends to 0.3 map units (about 20-80 base pairs) the distance at which a single base-pair substitution can exert such an effect. The presence of a …
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Probing Non-Canonical DNA Structures With Anticancer Drugs
T:G mismatched base pair is associated with many genetic mutations. Understanding its biological consequences can be aided by studying the structural perturbation of DNA caused by T:G base pair and by specific probing of the mismatch using small molecular ligands. We have shown that AR-1-144, a …
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Exploring cis-regulatory models of the genome to predict epigenetic state and variation
… rectifies the inability of previous sequence-based methods to generalize across cell-types. We permit generalization by introducing arbitrary base-pair resolution covariates. We show that the addition of base-pair resolution chromatin accessibility covariate greatly aids in the prediction of …
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Studies on the operator-repressor-effector interactions in the glp regulon of Escherichia Coli K-12
… Cooperativity was maximal when two 20-base pair operators were directly repeated and decreased with the deletion of 2 base pairs or the addition of 4 base pairs between the operators. Cooperative binding was eliminated by a 6 base pair insertion between tandem operators.
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The transcription factors Pax-6 and X-dll3 influence visual and olfactory system development in Xenopus laevis
… four distinct classes. These classes are defined based on the presence or absence of a 42 base pair sequence in the paired box, and the presence or absence of a 151 base pair sequence located downstream of the homeobox. Transcripts containing the 151 base pair sequence encode a highly conserved …
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Characterization of the Pichia pastoris alcohol oxidase I promoter
… to-235, and, -235 to -188. In addition, a 14-base pair internal deletion was made between positions, -215 to -201. This region was shown to be necessary for transcriptional activation by deletion analysis. Sufficiency studies suggested that this 14-base pair element could serve as an activator …
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Effects of a carcinogenic alkyl lesion on DNA: A structural study by x-ray crystallography
… A resolution. The two independent $\rm e\sp6G$:C base pairs in the DNA duplex adopt different base pairing schemes. A bifurcated base-pair configuration of $\rm e\sp6G$:C with three-centered hydrogen bonds mimicking a Watson-Crick pairing, a wobble configuration, as well as a ""reverse wobble"" …
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Chaotic Dynamics of Polyatomic Systems with an Emphasis on DNA Models
… a spectrum of temperatures, and the effect of base pair disorder on the dynamics is investigated. In addition to the inherent heterogeneity due to the proportion of adenine-thymine (AT) and guanine-cytosine (GC) base pairs, the distribution of these base pairs in the sequence is analysed …
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Biochemical Studies of the Purine Analogues, 2-Aminopurine and 2,6 Diaminopurine
… of helical DNA suffices to convert the A-T base pair from antibiotic indifference to antibiotic sensitivity. The second purine analogue studied in this thesis is 2-aminopurine. Like 2,6 diaminopurine, 2-aminopurine is a substrate analogue of only dATP with DNA polymerase. Also dAT polymers …
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Novel nucleotide analogues for forming stable DNA triple helices
… via specific recognition of the individual base pairs in the target sequence.<br/>Mixed-sequence recognition of duplex DNA by TFOs is therefore an essential requirement for successful targeting. However, achieving strong, yet specific binding to the pyrimidine.purine (Py.Pu) base pairs CG …
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Studies on approaches for sequencing cytosine modifications and the identification of their protein readers
… interest. A suite of sequencing methods allows base-resolution detection of all known cytosine modifications, but such approaches are often destructive and error-prone due to subtractive analysis of multiple experiments. Proteins binding cytosine modifications often interact with both marks in a …
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Investigation Into The Binding Interactions Of Klenow Fragment To Dna Modified With Carcinogens Af And Aaf Using Surface Plasmon Resonance
… the AF adduct is more easily bypassed, causing base substitutions. Surface plasmon resonance (SPR) was used to study the binding of exonuclease deficient E. coli polymerase I, Klenow fragment (KF), to DNA modified with AF or AAF at two locations: as a templating base or in the last formed base …
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Genome-Wide Dynamics of Chromatin Maturation Following DNA Replication
… methods, while informative, lack the genome-wide base-pair resolution required to assess the dynamics of chromatin assembly and maturation in a spatial-temporal manner. To overcome the limitations of these studies, I have taken advantage of an epigenome mapping technique based on micrococcal …
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Photoelectrochemical array platform for genomic scale DNA synthesis
… holds the potential for lowest cost per base pair of DNA produced over alternative microarray technologies, and may prove to be useful in de novo synthesis of large DNA constructs.
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