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Showing 1 to 20 of 20 for “"basal medium"”.
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Mechanisms of pathological adipogenesis
… was higher in 7F2 cells cultured with adipogenic medium compared with basal medium, indicating that 7F2 cells differentiated into adipocytes. 7F2 cells also expressed previously unreported variant isoforms of ghrelin at the mRNA level; these variants isoforms were much more abundant than native …
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Utilization of tissue culture methods and molecular markers for improvement of Solanum phureja Juz. & Buk.
… presence of silver thiosulfate (STS) in the MS basal medium did not affect the frequency of cells with 2x nuclei but increased the proportion of cells with 1x nuclei and decreased the proportion with 4x nuclei. Results indicated that STS lower the occurrence of endopolyploid nuclei. The …
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Studies on the uptake and fate of hemoglobin pinocytosed by rat embryo fibroblasts cultured in normal or low magnesium media
… fibroblasts were cultured in a modified Eagle's basal medium with a 10% calf serum supplement. The test substrate was in a concentration of 200 μg/ml. Accumulation and spike experiments were conducted to measure uptake and digestion. Turnover experiments measured rates of digestion of a …
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Peptides can be utilized as amino acid sources for protein accretion and cell proliferation by cultured animal cells
… methionine-free Dulbecco’s modified Eagle’s medium supplemented with 6% fetal bovine serum. The cell cultures were incubated for 72 h at 37°C in a humidified environment of 90% air : 10% CO₂ for C₂C₁₂ and ovine satellite cells or 95% air : 5% CO₂ for MAC-T cells. The basal medium contained …
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Fermentation studies on Clostridium acetabutylicum
… by Clostridium acetobutylicum in clostridial basal medium (CBM) , reinforced clostridial medium (RCM) , Leung and Robson media was undertaken with the aim of developing a partially defined laboratory medium which produced solvent yields comparable to the molasses fermentation medium (MFM). The …
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Studies in the physiology of the genus Bacillus
… of the organisms under investigation upon the basal medium. Where it was felt that a representative number of strains were examined, definite conclusions as to the ability of the organisms to dissimilate various carbohydrates are drawn. A key for the classification of the B. mesentericus group …
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In vitro milk protein secretion by explants of Holstein bull mammary tissue from two different genetic lines
… was explanted and cultured for up to 96 h in basal medium (B) which consisted of Media 199 supplemented with fetal calf serum and bovine insulin, or stimulatory medium (St), which was further supplemented with triiodothyronine, estradiol 17-B, hydrocortisone, and bovine prolactin (PRL). …
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Studies on the micropropagation of carob : Ceratonia siliqua L.
… use of half-strength Murashige and Skoog (MS) basal medium as the initiation medium were found to alleviate the negative effects of the explant exudate production and minimise explant browning. Seedling cultures were easily initiated by germinating immature carob seeds onto MS medium …
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Characterization of botryococcus sp. and identification of carbohydrates metabolic related enzymes toward lipid production
… extracellular metabolites excreted into the Bold basal medium were also analysed and identified through GC-MS. Enzymes involved in carbohydrate metabolism leading to lipid production in B. sp. under natural conditions were also identified through one-dimensional gel electrophoresis followed by …
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Effect of platelet factors and thrombin on multiplication of human bone marrow mesenchymal stem cells cultured in low and high oxygen level in vitro
… or dishes. The human mesenchymal stem cell basal medium (Lonza) was supplemented with adult human serum with or without PDF or with or without thrombin. High and low oxygen environment (Biospherix) was applied during expansion of hBMMSC. Results: Cultivation medium containing PDF in 5% …
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Influence of hormones on synthesis and secretion of milk proteins by mammary tissue from male and female cattle of beef and dairy breeds
… tissue was explanted and cultured for 96 h in basal medium (B) which contained hormones necessary for maintenance, or stimulatory medium (P), further supplemented with prolactin. Selected cultures were incubated for 24 h in B or P medium containing 3H-amino acids. Concentrations of non-labeled …
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Enhancing the Industrial Potential of Filamentous Cyanobacteria
… environment was optimized using BG11 as the basal medium supplemented with 0.5 g/L NaHCO3 as the carbon source. Subsequent directed evolution trials yielded 3 confirmed mutants with increased biofuel tolerance: Nostoc punctiforme ATCC 29133 with a 20% improvement in linalool tolerance, …
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Redutive Dehalogenation of Chlorinated Alkanes by Novel Bacteria at the PetroProcessor of Louisiana Inc. Superfund Site
… strains could be cultured in completely defined basal medium and were also able to dehalogenate a variety of other vicinally chlorinated alkanes including 1,1,2,2-tetrachloroethane, 1,1,2-trichloroethane, 1,2-dichloroethane, and 1,2-dichloropropane via dichloroelimination reactions. Chlorinated …
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Isolation, growth and epidemiology of campylobacter jejuni / coli
… were shown to detoxify rather than enrich the basal medium. A blood-free selective agar (BFSA) was produced by incorporating cephazolin (10 mg/i) and sodium deoxycholate (0.1%). This medium and the SBA gave similar isolation rates from faecal specimens when incubated at 42 0C for 42h but the …
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Nutritional requirements of Treponema denticola and Treponema vincentii
… and Treponema vincentii were grown in a medium supplemented with 0.4% (wt/vol) alpha globulin in place of whole serum. Other serum fractions did not support growth. The growth factors in alpha globulin were destroyed by trypsin and by lipase. Lipid extraction of alpha globulin showed that …
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Micropollutants biotransformation by two nitrifying communities enriched from biofilm of a nitrification trickling tower
… inoculated biofilm samples to two reactors using basal medium without adding organic carbon. Ammonium was supplied into one reactor upon depletion, and nitrite was supplied to the other reactor. In the first reactor, namely AOB/NOB enrichment reactor, both of AOB and NOB were expected to be …
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Somatic embryogenesis in southern and tropical pine species: Loblolly pine (Pinus taeda), Longleaf pine (P. palustris) and Oocarpa pine (P. oocarpa)
… the molecular level (Chapter V). By testing four basal media combined with different plant growth regulator combinations, we have established stable embryogenic cultures from high value families of P. taeda and P. palustris using the tissue culture medium 1218 (Pullman et al 2005) in combination …
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Optimization of Growth Parameters for Algal Regrowth Potential Experiments
… to proliferate in sealed culture vessels. Bold’s Basal Medium (BBM) was selected as the media due to its extensive use with freshwater algal strains. However, this medium can limit algal growth through nutrient limitation and it became necessary to augment the original recipe. 50mM of sodium …
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Metabolic pathways of Campylobacter fetus
… of the organisms was extremely slow when a basal medium (BM) was used. At a level of 0.5% carbon source in BM, the growth of 482 was stimulated by glucose, lactate and acetate; citrate showed no effect. The growth of PB1 was stimulated by lactate and acetate; glucose had no effect and …
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Modelling the early development of cartilage and bone in Mseleni Joint Disease using induced pluripotent stem cells
… protocols were tested: one with low glucose medium and one with standard glucose medium. Changes in the morphology, growth characteristics and expression of relevant CD markers were determined. The mesenchymal phenotype was confirmed by the positive qRT-PCR expression of CD73, 105 and 90. …