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Showing 1 to 20 of 41 for “"bacteriorhodopsin"”.
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Proton Translocation in the Bacteriorhodopsin Photocycle
From the above studies, we concluded that at low pH, the released proton is from the PRC as at neutral pH, but is delayed until the O to BR transition. The O to BR transition is rate-limited by the deprotonation of the PRC.
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Fourier transform infrared difference spectroscopy of bacteriorhodopsin
… to study the photocycle and proton pump of bacteriorhodopsin (bR). By applying this method to the study of native bR and bR with modified chromophores, we have confirmed and extended the earlier resonance Raman results concerning the configuration of the chromophore of bR and its …
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Proton release and the photocycle of bacteriorhodopsin
Bacteriorhodopsin (bR) is a light-driven proton pump. Over the last several years, much has been done in determining the structure and function of this protein; however, the exact mechanism of the proton pump is still unknown. My work presented here focuses on characterizing the proton release …
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Molecular dynamics studies of the protein bacteriorhodopsin
Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T15:00:54Z Item is restricted indefinitely.
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Molecular dynamics studies of the protein bacteriorhodopsin
… study the structure and function of the protein bacteriorhodopsin (bR), a 26 kD protein which residues in the purple membrane of the bacterium Halobacterium halobium. Bacteriorhodopsin undergoes a light-driven cyclic process, which pumps protons across the membrane, in order to maintain a proton …
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Solid-state NMR investigations of the bacteriorhodopsin photocycle
Bacteriorhodopsin (bR) is a heptahelical integral membrane protein with a retinylidene prosthetic group from H. salinarum. The protein creates a proton gradient across the cell membrane by harvesting light energy. Proton transfer events can be segregated into transfers between the Schiff base of …
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Photoelectric Characterization of Bacteriorhodopsin Reconstituted in Lipid Bilayer Membrane
… conversion solutions. A photosynthetic protein (Bacteriorhodopsin) was reconstituted in a bi-layer membrane. Then, when a laser beam was shined on the membrane, the photon energy was used by the protein to pump protons across the membrane. The translocation of protons across the membrane was …
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FTIR study of carboxylic acids and tyrosines in bacteriorhodopsin
High quality infrared difference spectra of bacteriorhodopsin (bR) were obtained in order to study its photoreaction at the molecular level. The noise level was near 10-5 in the spectral regions where amino acid residues are monitored. The excellent signal to noise ratio allowed detection of …
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Molecular Dynamics and Quantum Chemistry Studies of the Protein Bacteriorhodopsin
… study the structure and function of the protein bacteriorhodopsin (bR), a 26 kD protein which residues in the purple membrane of the bacterium Halobacterium halobium. Bacteriorhodopsin undergoes a light-driven cyclic process, which pumps protons across the membrane, in order to maintain a proton …
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Relationships Between Proton Translocation, Absorption Changes, and Photocurrents in the Bacteriorhodopsin Photocycle
The mechanisms of proton release and uptake in bacteriorhodopsin (bR) were investigated using steady state and kinetic spectrophotometry and photocurrent methods. First, a kinetic model for the formation of the crucial M intermediate of the photocycle was formulated. The proposed model involved the …
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Studies on the Structure and Photochemistry of Bacteriorhodopsin (Proton Pumping, Cation Binding, Synthetic Pigments)
Cation Binding By Bacteriorhodopsin (bR). bR normally has a purple color ((lamda)(,max) = 558 nm), hence the name, purple membrane. Atomic absorption spectrophotometry shows that purple membrane binds approximately 4 Ca('++) and Mg('++) per bR at pH = 6.0. Ca('++) and Mg('++) can be removed by …
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Functional and time-resolved structural studies of bacteriorhodopsin mutants following expression in Halobacterium halobium
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 1995.
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Elucidating the pumping mechanism of bacteriorhodopsin using dynamic nuclear polarization enhanced magic angle spinning NMR
Bacteriorhodopsin (bR) is comprised of 7 trans-membrane helices that enclose a retinylidene chromophore formed by a Schiff base (SB) between the retinal and Lys216. Due to bR's relative availability, it serves as a model for other members of the rhodopsin family, ion channels and GPCRs. Since its …
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A Deuterium Nuclear Magnetic Resonance Study of Amino Acid Dynamics in the Membrane Protein, Bacteriorhodopsin (Nmr)
… incorporated them into the membrane protein, bacteriorhodopsin in the photosynthetic purple membrane of Halobacterium halobium. For L{(beta)-('2)H(,3)} alanine, DL{(gamma)-('2)H(,6)} valine, DL{(beta),(gamma)-('2)H(,4)} threonine, L{(delta)-('2)H(,3)} leucine, …
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Cation effects on the purple membrane of Halobacterium halobium
… related to the blue-to-purple transition of bacteriorhodopsin; whereas, four other divalent cation binding sites involving carboxyl groups on the bacteriorhodopsin surface do not directly affect the color change. The intrinsic pK$\sb{\rm a}$ of the binding of the special divalent cation and …
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